Physical and biological properties of compound membranes incorporating a copolymer with a phosphorylcholine head group

Zhang, S.F.; Rolfe, P. F.; Wright, G.; Wang, Lian; Milling, Andrew; Tanaka, Shinji; Ishihara, Kazuaki

doi:10.1016/s0142-9612(97)00174-9

Cited by 88 publications

(71 citation statements)

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“…Then, this surface is modified so that only a specific species of molecules can attach to it through a linker in a desired orientation. A membrane surface with zwitterionic polar head groups such as phosphatidyl choline (PC) and phosphatidyl ethanolamine (PE) is known to resist protein adsorption [189,190]. Streptavidin is also useful for the specific attachment of a biotinylated protein since it also resists nonspecific protein adsorption.…”

Section: Substrata For Observing Dynamic Biomolecular Processesmentioning

confidence: 99%

High-speed atomic force microscopy for nano-visualization of dynamic biomolecular processes

Ando

Uchihashi

Fukuma

2008

Progress in Surface Science

485

401

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The atomic force microscope (AFM) has a unique capability of allowing the high-resolution imaging of biological samples on substratum surfaces in physiological solutions. Recent technological progress of AFM in biological research has resulted in remarkable improvements in both the imaging rate and the tip force acting on the sample. These improvements have enabled the direct visualization of dynamic structural changes and dynamic interactions occurring in individual biological macromolecules, which is currently not possible with other techniques. Therefore, high-speed AFM is expected to have a revolutionary impact on biological sciences. In addition, the recently achieved atomic resolution in liquids will further expand the usefulness of AFM in biological research. In this article, we first describe the various capabilities required of AFM in biological sciences, which is followed by a detailed description of various devices and techniques developed for high-speed AFM and atomic-resolution in-liquid AFM. We then describe various imaging studies performed using our cutting-edge microscopes and their current capabilities as well as their limitations, and conclude by discussing the future prospects of AFM as an imaging tool in biological research.

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Section: Substrata For Observing Dynamic Biomolecular Processesmentioning

confidence: 99%

High-speed atomic force microscopy for nano-visualization of dynamic biomolecular processes

Ando

Uchihashi

Fukuma

2008

Progress in Surface Science

485

401

View full text Add to dashboard Cite

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“…Much lower adsorption of BSA onto copolymer 1 (9.5 ng·cm −2 compared to 74.41 ng·cm −2 , 80.70 ng·cm −2 and 106.25 ng·cm −2 for the sensors coated with copolymer 2, copolymer 3 and bare sensor, respectively) indicated excellent antifouling properties of this zwitterionic chemistry (p(85% 4-VP-co-EGDA). It has been both theoretically and experimentally accepted that higher zwitterionic content helps formation of a hydration layer (due to higher electrostatic interaction with ambient water), which helps reduce fouling of surfaces [21,39].…”

Section: Fouling Resistance Testing Of Zwitterionic Coatingmentioning

confidence: 99%

“…The most common of these techniques include self-assembled monolayer (SAM) formation [7], solution polymerization with solvent evaporation [20,21] and atom transfer radical polymerization (ATRP) [22][23][24][25]. However, these methods have several limitations, e.g., SAMs require specific surface functionality, which limits this method to gold substrates.…”

Section: Introductionmentioning

confidence: 99%

Surface modification of reverse osmosis membranes with zwitterionic coating for improved resistance to fouling

et al. 2015

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“…Then, this surface is modified so that only a specific species of molecules can attach to it through a linker in a desired orientation. A membrane surface with zwitterionic polar head groups such as phosphatidyl choline (PC) and phosphatidyl ethanolamine (PE) is known to resist protein adsorption [87,88]. Streptavidin is also useful for specific attachment of biotinylated protein because it also resists nonspecific protein adsorption.…”

Section: Substratummentioning

confidence: 99%

High-speed AFM and nano-visualization of biomolecular processes

Ando

Uchihashi

Kodera

et al. 2007

Pflugers Arch - Eur J Physiol

233

172

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Conventional atomic force microscopes (AFMs) take at least 30-60 s to capture an image, while dynamic biomolecular processes occur on a millisecond timescale or less. To narrow this large difference in timescale, various studies have been carried out in the past decade. These efforts have led to a maximum imaging rate of 30-60 ms/ frame for a scan range of~250 nm, with a weak tip-sample interaction force being maintained. Recent imaging studies using high-speed AFM with this capacity have shown that this new microscope can provide straightforward and prompt answers to how and what structural changes progress while individual biomolecules are at work. This article first compares high-speed AFM with its competitor (single-molecule fluorescence microscopy) on various aspects and then describes high-speed AFM instrumentation and imaging studies on biomolecular processes. The article concludes by discussing the future prospects of this cutting-edge microscopy.

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Physical and biological properties of compound membranes incorporating a copolymer with a phosphorylcholine head group

Cited by 88 publications

References 0 publications

High-speed atomic force microscopy for nano-visualization of dynamic biomolecular processes

High-speed atomic force microscopy for nano-visualization of dynamic biomolecular processes

Surface modification of reverse osmosis membranes with zwitterionic coating for improved resistance to fouling

High-speed AFM and nano-visualization of biomolecular processes

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