Physical and biological engineering of polymer scaffolds to potentiate repair of spinal cord injury

Luo, Yiqian; Xue, Fei; Li, Kai; Li, Baoqin; Fu, Changfeng; Ding, Jianxun

doi:10.1016/j.matdes.2021.109484

Cited by 35 publications

(25 citation statements)

References 155 publications

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“…The same concept is generally applicable to the injured spinal cord, where the goal is to reconstruct the relevant geometry in a way that best mimics the cytoarchitecture of the normal white matter tracts of the spinal cord. Attempts to develop suitable materials for this purpose include a wide variety of approaches ranging from cell-based therapies to the implantation of artificial scaffolds with specific architecture and combinations of both approaches [152][153][154][155][156][157][158][159][160]. Although most publications in material science do not cite the experimental evidence supporting the primary role of tissue geometry, the approaches that have been taken are largely based on the assumption that appropriate geometry is critical for promoting successful regeneration both in the PNS and CNS.…”

Section: Reconstruction Of Tissue Geometry To Promote Regenerationmentioning

confidence: 99%

“…There have been numerous subsequent studies that have used a wide variety of materials to explore what topographical or biochemical features are best suited to support directional growth of neurites as well as the alignment of glial cells that, in turn, would support such growth. This literature is extensive and has been reviewed by several investigators [154,156,157,166,167]. One of the overarching conclusions that can be gleaned from these studies is that aligned substrates, whatever means by which they are produced, exert compelling directional cues for growing neurites.…”

Section: Reconstruction Of Tissue Geometry To Promote Regenerationmentioning

confidence: 99%

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The Role of Tissue Geometry in Spinal Cord Regeneration

et al. 2022

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Unlike peripheral nerves, axonal regeneration is limited following injury to the spinal cord. While there may be reduced regenerative potential of injured neurons, the central nervous system (CNS) white matter environment appears to be more significant in limiting regrowth. Several factors may inhibit regeneration, and their neutralization can modestly enhance regrowth. However, most investigations have not considered the cytoarchitecture of spinal cord white matter. Several lines of investigation demonstrate that axonal regeneration is enhanced by maintaining, repairing, or reconstituting the parallel geometry of the spinal cord white matter. In this review, we focus on environmental factors that have been implicated as putative inhibitors of axonal regeneration and the evidence that their organization may be an important determinant in whether they inhibit or promote regeneration. Consideration of tissue geometry may be important for developing successful strategies to promote spinal cord regeneration.

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Section: Reconstruction Of Tissue Geometry To Promote Regenerationmentioning

confidence: 99%

Section: Reconstruction Of Tissue Geometry To Promote Regenerationmentioning

confidence: 99%

The Role of Tissue Geometry in Spinal Cord Regeneration

et al. 2022

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“…Given that the spinal cord is a highly hydrated soft “material”, hydrogels with physicochemical and mechanical properties similar to natural extracellular matrix showing significant advantages over stiff scaffolds, e.g. the risk of tissue damage due to mechanical mismatch could be well avoided [ [11] , [12] , [13] , [14] ]. They could be further tailored to be injectable, which would completely fill the cavity in a noninvasive manner, regardless of the size and shape [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

An injectable and self-healing hydrogel with controlled release of curcumin to repair spinal cord injury

Luo

Shi

et al. 2021

Bioactive Materials

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The harsh local micro-environment following spinal cord injury (SCI) remains a great challenge for neural regeneration. Local reconstitution of a favorable micro-environment by biocompatible scaffolds with desirable functions has thus been an area of concern. Herein, a hybrid hydrogel was developed using Fmoc-grafted chitosan (FC) and Fmoc peptide (FI). Dynamic reversible π-π stacking interactions of the fluorenyl rings enabled the FC/FI hybrid hydrogel to exhibit excellent injectable and self-healing properties, as characterized by visual appearances and rheological tests. Furthermore, the FC/FI hybrid hydrogel showed a slow and persistent release of curcumin (Cur), which was named as FC/FI-Cur hydrogel. In vitro studies confirmed that with the support of FC/FI-Cur hydrogel, neurite outgrowth was promoted, and Schwann cell (SC) migration away from dorsal root ganglia (DRG) spheres with enhanced myelination was substantiated. The FC/FI-Cur hydrogel well reassembled extracellular matrix at the lesion site of rat spinal cord and exerted outstanding effects in modulating local inflammatory reaction by regulating the phenotypes of infiltrated inflammatory cells. In addition, endogenous SCs were recruited in the FC/FI-Cur graft and participated in the remyelination process of the regenerated nerves. These outcomes favored functional recovery, as evidenced by improved hind limbs movement and enhanced electrophysiological properties. Thus, our study not only advanced the development of multifunctional hydrogels but also provided insights into comprehensive approaches for SCI repair.

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“…The scaffolds currently studied were developed to respond to various specific-tissued properties, such as mechanical support and porosity formation [ 1 ]. Nowadays, scaffold techniques are available to make them more effective in molding and material selection [ 2 , 3 ]. 3D printing is the processing of a workpiece by injecting a material into the shape designed by a computer program [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of 3D-Printing Scaffold Fabrication on Biosynthetic Medium-Chain-Length Polyhydroxyalkanoate Terpolyester as Biomaterial-Ink

Panaksri

Tanadchangsaeng

2021

Polymers

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Currently, the selection of materials for tissue engineering scaffolds is still limited because some tissues require flexible and compatible materials with human cells. Medium-chain-length polyhydroxyalkanoate (MCL-PHA) synthesized in microorganisms is an interesting polymer for use in this area and has elastomeric properties compatible with the human body. MCL-PHAs are elastomers with biodegradability and cellular compatibility, making them an attractive material for fabricating soft tissue that requires high elasticity. In this research, MCL-PHA was produced by fed-batch fermentation that Pseudomonas Putida ATCC 47054 was cultured to accumulate MCL-PHA by using glycerol and sodium octanoate as carbon sources. The amounts of dry cell density, MCL-PHA product per dry cells, and MCL-PHA productivity were at 15 g/L, 27%, and 0.067 g/L/h, respectively, and the components of MCL-PHA consisting of 3-hydroxydecanoate (3HD) 64.5%, 3-hydroxyoctanoate (3HO) 32.2%, and 3-hydroxyhexanoate (3HHx) 3.3%. The biosynthesized MCL-PHA terpolyester has a relatively low melting temperature, low crystallinity, and high ductility at 52 °C, 15.7%, and 218%, respectively, and considering as elastomeric polyester. The high-resolution scaffold of MCL-PHA terpolyester biomaterial-ink (approximately 0.36 mm porous size) could be printed in a selected condition with a 3D printer, similar to the optimum pore size for cell attachment and proliferation. The rheological characteristic of this MCL-PHA biomaterial-ink exhibits shear-thinning behavior, leading to good shape fidelity. The study results yielded a condition capable of fabricating an elastomer scaffold of the MCL-PHA terpolyester, giving rise to the ideal soft tissue engineering application.

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Physical and biological engineering of polymer scaffolds to potentiate repair of spinal cord injury

Cited by 35 publications

References 155 publications

The Role of Tissue Geometry in Spinal Cord Regeneration

The Role of Tissue Geometry in Spinal Cord Regeneration

An injectable and self-healing hydrogel with controlled release of curcumin to repair spinal cord injury

Evaluation of 3D-Printing Scaffold Fabrication on Biosynthetic Medium-Chain-Length Polyhydroxyalkanoate Terpolyester as Biomaterial-Ink

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