Physical and biological advances in endothelial cell-based engineered co-culture model systems

Mierke, Claudia Tanja

doi:10.1016/j.semcdb.2023.01.012

Cited by 9 publications

(5 citation statements)

References 175 publications

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“…Based on the differences in hGF monolayers and 3D gingiva culture models, it is evident that the use of 3D models could be necessary for studying the effects of specific molecules. Although the interaction of immune system cells is still missing, their use provides a higher degree of complexity closer to the in vivo situation [ 45 , 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

A Sequential Micro-Immunotherapy Medicine Increases Collagen Deposition in Human Gingival Fibroblasts and in an Engineered 3D Gingival Model under Inflammatory Conditions

Ferrà-Cañellas

Munar-Bestard

Floris³

et al. 2023

IJMS

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Periodontal therapies use immune mediators, but their side effects can increase with dosage. Micro-immunotherapy (MI) is a promising alternative that employs immune regulators at low and ultralow doses to minimize adverse effects. In this study, the effects of 5 capsules and the entire 10-capsule sequence of the sequential MI medicine (MIM-seq) were tested in two in vitro models of periodontitis. Firstly, human gingival fibroblasts (hGFs) exposed to interleukin (IL)-1β to induce inflammation were treated with five different capsules of MIM-seq for 3 days or with MIM-seq for 24 days. Subsequently, MIM-seq was analyzed in a 3D model of human tissue equivalent of gingiva (GTE) under the same inflammatory stimulus. Simultaneously, a non-IL-1β-treated control and a vehicle were included. The effects of the treatments on cytotoxicity, collagen deposition, and the secreted levels of IL-1α, IL-6, prostaglandin E2 (PGE2), matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of metalloproteinases-1 (TIMP-1) were evaluated. None of the tested items were cytotoxic. The complete sequence of MIM-seq decreased PGE2 release and restored collagen deposition levels induced by IL-1β treatment in hGFs exposed to IL-1β. MIM-seq treatment restored collagen production levels in both models. These promising preclinical findings suggest that MIM-seq should be further investigated for periodontitis treatment.

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Section: Discussionmentioning

confidence: 99%

A Sequential Micro-Immunotherapy Medicine Increases Collagen Deposition in Human Gingival Fibroblasts and in an Engineered 3D Gingival Model under Inflammatory Conditions

Ferrà-Cañellas

Munar-Bestard

Floris³

et al. 2023

IJMS

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“…Organoids provide a biologically meaningful stage for enhancing translatability. Cocultures represent no novel concept in the experimental work, as they are frequently utilized to investigate interferences between epithelial cells and other relevant cell populations, for example, lymphocytes, neurons, and blood vessels, as recently reviewed in [66]. The cultivation of epithelial cancer organoids with immune cells has provided valuable findings on the pathogenesis of various cancers, and the opportunity to genetically engineer these types of organoids in the absence or presence of immune cells offers a distinct and pertinent model for the examination of carcinogenesis [67][68][69].…”

Section: Universal Properties Of Cancer and Their Effect On Cancer Ce...mentioning

confidence: 99%

Phenotypic Heterogeneity, Bidirectionality, Universal Cues, Plasticity, Mechanics, and the Tumor Microenvironment Drive Cancer Metastasis

Mierke

2024

Biomolecules

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Tumor diseases become a huge problem when they embark on a path that advances to malignancy, such as the process of metastasis. Cancer metastasis has been thoroughly investigated from a biological perspective in the past, whereas it has still been less explored from a physical perspective. Until now, the intraluminal pathway of cancer metastasis has received the most attention, while the interaction of cancer cells with macrophages has received little attention. Apart from the biochemical characteristics, tumor treatments also rely on the tumor microenvironment, which is recognized to be immunosuppressive and, as has recently been found, mechanically stimulates cancer cells and thus alters their functions. The review article highlights the interaction of cancer cells with other cells in the vascular metastatic route and discusses the impact of this intercellular interplay on the mechanical characteristics and subsequently on the functionality of cancer cells. For instance, macrophages can guide cancer cells on their intravascular route of cancer metastasis, whereby they can help to circumvent the adverse conditions within blood or lymphatic vessels. Macrophages induce microchannel tunneling that can possibly avoid mechanical forces during extra- and intravasation and reduce the forces within the vascular lumen due to vascular flow. The review article highlights the vascular route of cancer metastasis and discusses the key players in this traditional route. Moreover, the effects of flows during the process of metastasis are presented, and the effects of the microenvironment, such as mechanical influences, are characterized. Finally, the increased knowledge of cancer metastasis opens up new perspectives for cancer treatment.

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“…The hollow collagen gels obtained by the one-pot method should be very interesting for fabricating a novel 3D co-culture system. Co-culture systems can reconstruct more biomimetic environments than monoculture systems [ 41 , 42 , 43 ], such as promoting tissue formation through direct or indirect cellular interactions [ 44 ]. Micropatterned co-culture systems have been reported to localize and coexist with multiple cells in optimal spaces [ 45 ], thereby controlling the level of cell-cell interactions [ 46 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of UV Irradiation of Pre-Gel Solutions on the Formation of Collagen Gel Tubes

Ishibashi

Haraguchi

Aoki

et al. 2023

Gels

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Hollow collagen gels are promising materials for drug/cell delivery systems to promote tissue regeneration because they may be able to function as carriers for these types of loads. Controlling the cavity size and swelling suppression is essential to expand the applications and improve the usability of such gel-like systems. We investigated the effects of UV-treated collagen solutions as a pre-gel aqueous mixture on the formation and properties of the hollow collagen gels in terms of their preparation range limits, morphology, and swelling ratio. The UV treatment thickened the pre-gel solutions, which allowed hollowing at lower collagen concentrations. This treatment also prevents the over-swelling of the hollow collagen rods in PBS buffer solutions. The UV-treated collagen solutions provided a large lumen space in the prepared collagen hollow fiber rods with a limited swelling ratio, allowing vascular endothelial cells and ectodermal cells to be cultured separately in the outer and inner lumen.

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Physical and biological advances in endothelial cell-based engineered co-culture model systems

Cited by 9 publications

References 175 publications

A Sequential Micro-Immunotherapy Medicine Increases Collagen Deposition in Human Gingival Fibroblasts and in an Engineered 3D Gingival Model under Inflammatory Conditions

A Sequential Micro-Immunotherapy Medicine Increases Collagen Deposition in Human Gingival Fibroblasts and in an Engineered 3D Gingival Model under Inflammatory Conditions

Phenotypic Heterogeneity, Bidirectionality, Universal Cues, Plasticity, Mechanics, and the Tumor Microenvironment Drive Cancer Metastasis

Effect of UV Irradiation of Pre-Gel Solutions on the Formation of Collagen Gel Tubes

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