Phylogeography of Biomphalaria glabrata and B. pfeifferi, important intermediate hosts of Schistosoma mansoni in the New and Old World tropics

DeJong, Randall J.; Morgan, Jess A. T.; Wilson, Wade D.; Al-Jaser, Mai H.; Appleton, C.C.; Coulibaly, G.; D’Andrea, Paulo Sérgio; Doenhoff, Michael J.; Haas, Wilfried; Idris, Mohamed A.; Magalhães, Luiz Augusto; Moné, Hélène; Mouahid, Gabriel; Mubila, Likezo; Pointier, J. P.; Webster, Joanne P.; Zanotti-Magalhães, Eliana Maria; Paraense, W. Lobato; Mkoji, Gerald M.; Loker, Eric S.

doi:10.1046/j.1365-294x.2003.01977.x

Cited by 51 publications

(52 citation statements)

References 86 publications

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“…4, the PCR product (272 bp) corresponding to the expected size of the nimbus (BgI) cDNA transcript (Bg37) product was amplified from DNA from all the snails examined. The uniform distribution of this retrotransposon in all these snails, including phylogenetically distant Pomatiopsidae Oncomelania and more recent Basommatophora snails (Biomphalaria and Bulinus), provides evidence (Dejong et al, 2003) of an ancient colonization of the genomes of these snails by this L1-like retrotransposon.…”

Section: The L1 Related Retrotransposon Is Widely Distributed Among Smentioning

confidence: 90%

Nimbus (BgI): An active non-LTR retrotransposon of the Schistosoma mansoni snail host Biomphalaria glabrata

Raghavan

Tettelin²,

Miller

et al. 2007

International Journal for Parasitology

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The freshwater snail Biomphalaria glabrata is closely associated with the transmission of human schistosomiasis. An ecologically sound method has been proposed to control schistosomiasis using genetically modified snails to displace endemic, susceptible ones. To assess the viability of this form of biological control, studies towards understanding the molecular makeup of the snail relative to the presence of endogenous mobile genetic elements are being undertaken since they can be exploited for genetic transformation studies. We previously cloned a 1.95 Kb BamHI fragment in B. glabrata (BGR2) with sequence similarity to the human long interspersed nuclear element (LINE or L1). A contiguous, full-length sequence corresponding to BGR2, hereafter-named nimbus (BgI), has been identified from a B. glabrata bacterial artificial chromosome (BAC) library. Sequence analysis of the 65,764 bp BAC insert contained one full-length, complete nimbus (BgI) element (element I), two full-length elements (elements II and III) containing deletions and flanked by target site duplications and 10 truncated copies. The intact nimbus (BgI) contained two open reading frames (ORFs 1 and 2) encoding the characteristic hallmark domains found in non-long terminal repeat retrotransposons belonging to the I clade; a nucleic acid binding protein in ORF1 and an apurinic/ apyrimidinic endonuclease, reverse transcriptase and RNase H in ORF2. Phylogenetic analysis revealed that nimbus (BgI) is closely related to Drosophila (I factor), mosquito Aedes aegypti (MosquI) and chordate ascidian Ciona intestinalis (CiI) retrotransposons. Nimbus (BgI) represents the first complete mobile element characterized from a mollusk that appears to be transcriptionally active and is widely distributed in snails of the neotropics and the Old World.

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Section: The L1 Related Retrotransposon Is Widely Distributed Among Smentioning

confidence: 90%

Nimbus (BgI): An active non-LTR retrotransposon of the Schistosoma mansoni snail host Biomphalaria glabrata

Raghavan

Tettelin²,

Miller

et al. 2007

International Journal for Parasitology

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“…The distribution of B. glabrata largely defines the areas that are endemic for schistosomiasis in South and Central America (Dejong et al, 2003), but only a low proportion of snails in the field is patently infected with schistosomes (de Souza et al, 1994) and some B. glabrata are naturally resistant (Paraense and Correa, 1963). Increasingly, experimental study of B. glabrata as intermediate host of schistosomes and related digenetic trematode parasites incorporates characterization of the genome (Adema et al, 2006;DeJong et al, 2004;Raghavan and Knight, 2006), the proteome (Bouchut et al, 2006b;Vergote et al, 2005) and especially the transcriptome (Bouchut et al, 2006a;Jung et al, 2005;Knight et al, 1999;Lockyer et al, 2007a;Lockyer et al, 2007b;Miller et al, 2001;Mitta et al, 2005;Nowak et al, 2004;Raghavan et al, 2003) …”

Section: Introductionmentioning

confidence: 99%

Comparative ORESTES-sampling of transcriptomes of immune-challenged Biomphalaria glabrata snails

Hanelt

Lun

Adema

2008

Journal of Invertebrate Pathology

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The snail Biomphalaria glabrata (Gastropoda, Mollusca) is an important intermediate host for the human parasite Schistosoma mansoni (Digenea, Trematoda). Anti-pathogen responses of B. glabrata were studied towards a better understanding of snail immunity and host-parasite compatibility. Open reading frame ESTs (ORESTES) were sampled from different transcriptomes of M line strain B. glabrata, 12 hours post challenge with Escherichia coli (Gram-negative), Micrococcus luteus (Gram-positive) bacteria or compatible S. mansoni, and controls. The resulting 3123 ORESTES represented 2129 unique sequences (373 clusters, 1756 singletons). Of these, 175 (8.1%) were putative defense factors, including lectins, antimicrobial peptides and components of various immune effector systems. Comparison of biological processes (GO-terms) within different transcriptomes indicated that B. glabrata increased oxygen transport and metal binding in reaction to all challenges. Comprehensive comparisons of transcriptomes revealed that responses of B. glabrata against bacteria were similar to each other and differed from the ineffective response to S. mansoni. Furthermore, the response to S. mansoni infection was less comprehensive than that to bacteria. Many novel (unknown) sequences were recovered in association with particular challenges. Biomphalaria glabrata possesses multi-faceted, potent immune defenses. This agrees with the notion that S. mansoni is capable of immune-evasion and prevents effective host defense responses in order to survive in B. glabrata. Future analysis of the numerous unknown sequences recovered from challenged snails may reveal novel immune factors and provide increased understanding of immunity of B. glabrata in relation to parasite-host compatibility.

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“…(R=A/G Y=C/T H= A/C/T). The sequences from these amplicons were obtained by direct sequencing and analyzed relative to previously generated phylogenies of Biomphalaria isolates, all according to DeJong et al (2003). The sequences of 16S rDNA and NADH dehydrogenase 1 were deposited in GenBank under accession numbers AY737280 and AY737281, respectively.…”

mentioning

confidence: 99%

A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni

Adema

Luo

Hanelt

et al. 2006

Mem. Inst. Oswaldo Cruz

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To provide a novel resource for analysis of the genome of Biomphalaria glabrata, members of the international Biomphalaria glabrata Genome Initiative (biology.unm.edu/biomphalaria-genome.html Key words: genomics -gene discovery -fingerprinting -schistosomiasis -medical malacologyThe application of molecular approaches continues to contribute novel insights into the biology, including genomics of molluscs . To date, several mitochondrial genomes of molluscs have been sequenced (DeJong et al. 2004, Mizi et al. 2005, but the nuclear genome of a representative of the Phylum Mollusca remains to be fully characterized. In fact, lophotrochozoan protostomes of which mollusca represent the largest group (Rouse 1999), are underrepresented among the animals from which the current assembly of fully sequenced genomes has been obtained. Thus, genomic data from a mollusc will help fill a gap in the information on the evolutionary history of animal life (Collins et al. 2003). Molluscs are a highly diverse group that includes some of the largest, longest living, and most intelligent invertebrates. Genome information will instruct on several remarkable properties of molluscs such as shell formation (biomineralization; Milet et al. 2004), the evolution of body asymmetry (Schilthuizen & Davison 2005), and hermaphrodism (Paraense & Corrêa 1988). Molluscs are also being used to study pharmo-toxicology (Terlau & Olivera 2004); neuroendocrinology (Altelaar et al. 2005); parthenogenesis (Jokela et al. 2003); and the molecular basis of behavior and learning (Williamson & Chrachri 2004, Zhurov et al. 2005. Molluscs serve as bioindicators for monitoring of the environment (Zhao et al. 2005), and (snails especially) are useful for understanding how natural selection operates (Vermeij 2002). Furthermore, molluscs are economically important as a major source of food, can destroy crops, colonize and impact new habitats as invasive species (Pointier et al. 2005), and transmit medically important pathogens.The latter applies to the freshwater gastropod Biomphalaria glabrata (Planorbidae, Basommatophora). This snail serves as one of the most important intermediate hosts for a widespread pathogen of humans, the digenetic trematode Schistosoma mansoni (Paraense & Corrêa 1963, Morgan et al. 2001). This parasite causes intestinal schistosomiasis, a debilitating disease that afflicts over 50 million humans (Chitsulo et al. 2004). To a large extent, the geographic distribution of B. glabrata defines the et al. 2004). B. glabrata also hosts a variety of other digenetic trematodes and has been adopted as the most commonly used model host to study the basic biology of digeneansnail interactions (Lie 1982, Adema & Loker 1997, Vergote et al. 2005. As one example, B. glabrata has been found to produce after exposure to digeneans a unique family of hemolymph molecules termed FREPs (fibrinogen-related proteins). FREPs consist of a juxtaposition of fibrinogen and immunoglobulin superfamily domains, and have proven to be remarkably diverse in their composition...

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Phylogeography of Biomphalaria glabrata and B. pfeifferi, important intermediate hosts of Schistosoma mansoni in the New and Old World tropics

Cited by 51 publications

References 86 publications

Nimbus (BgI): An active non-LTR retrotransposon of the Schistosoma mansoni snail host Biomphalaria glabrata

Nimbus (BgI): An active non-LTR retrotransposon of the Schistosoma mansoni snail host Biomphalaria glabrata

Comparative ORESTES-sampling of transcriptomes of immune-challenged Biomphalaria glabrata snails

A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni

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