Phylogeography and molecular epidemiology of Papaya ringspot virus

Castillo, X.A. Olarte; Fermín, Gustavo; Tabima, Javier F.; Rojas, Yndra Cordero de; Tennant, Paula; Fuchs, Marc; Sierra, Roberto; Bernal, Adriana; Restrepo, Silvia

doi:10.1016/j.virusres.2011.04.011

Cited by 66 publications

(42 citation statements)

References 56 publications

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“…Phylogenetic studies suggest that PRSV originated from India and spread to Asia and the New World about 400 yrs ago (Gibbs et al, 2008a;Olarte-Castillo et al, 2011). ZTMV has been reported from three continents and some Caribbean and Indian Ocean islands (Romay et al, 2014).…”

Section: Geographic Distribution and Evolution Of Prsv-like Virusesmentioning

confidence: 99%

New species in the papaya ringspot virus cluster: Insights into the evolution of the PRSV lineage

Desbiez¹,

Wipf-Scheibel²,

Millot³

et al. 2017

Virus Research

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Please cite this article as: Desbiez, C., Wipf-Scheibel, C., Millot, P., Verdin, E., Dafalla, G., Lecoq, H., New species in the papaya ringspot virus cluster: insights into the evolution of the PRSV lineage.Virus Research http://dx.doi.org/10. 1016/j.virusres.2017.06.022 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. SuWMV appeared 10 times more frequent than WMVBV in that country (14.6% vs. 1.5% of the samples tested). The geographic structure and molecular diversity patterns of the putative and acknowledged species suggest that the PRSV-like cluster originated in the Old World about 3600 years ago, with an important diversification in Africa.

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Section: Geographic Distribution and Evolution Of Prsv-like Virusesmentioning

confidence: 99%

New species in the papaya ringspot virus cluster: Insights into the evolution of the PRSV lineage

Desbiez¹,

Wipf-Scheibel²,

Millot³

et al. 2017

Virus Research

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show abstract

“…For some authors, this prevalence may be influenced by biotic and abiotic factors and the high activity of the potyvirus for cucurbits (IBABA et al, 2015). Studies conducted in different regions of Brazil have shown high prevalence of PRSV-W and ZYMV in cucurbits, followed by Olarte Castillo et al (2011).…”

Section: Resultsmentioning

confidence: 99%

Susceptibility assessment of watermelon and pumpkin cultivars to potyvirus in Tocantins

Ootani¹,

Aguiar²,

Maciel³

et al. 2017

Biosci. J.

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“…Th e probebased systems such as TaqMan real time PCR can reduce background and false positives because probes are specifi c for their targets (Papin et al, 2004;Richards et al, 2004). However, viruses including PRSV and PLDMV have generally the high level of genetic diversity in sequences among the diff erent strains (Akhter et al, 2013;Lu et al, 2008;Olarte Castillo et al, 2011;Tuo et al, 2013), the probe-based real time RT-PCR assays require high sequence identity for successful probe binding that frequently leads to false negative results and loss of sensitivity (Papin et al, 2004;Richards et al, 2004). Th erefore, dyes as detection tools can be more suitable to detect the virus infection because they are potentially less infl uenced by mismatches for sequence variation in the probe target region.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous detection of papaya ringspot virus, papaya leaf distortion mosaic virus, and papaya mosaic virus by multiplex real-time reverse transcription PCR

Huo¹,

Shen²,

Yan³

et al. 2015

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Summary. -Both the single infection of papaya ringspot virus (PRSV), papaya leaf distortion mosaic virus (PLDMV) or papaya mosaic virus (PapMV) and double infection of PRSV and PLDMV or PapMV which cause indistinguishable symptoms, threaten the papaya industry in Hainan Island, China. In this study, a multiplex real-time reverse transcription PCR (RT-PCR) was developed to detect simultaneously the three viruses based on their distinctive melting temperatures (Tms): 81.0 ± 0.8°C for PRSV, 84.7 ± 0.6°C for PLDMV, and 88.7 ± 0.4°C for PapMV. Th e multiplex real-time RT-PCR method was specifi c and sensitive in detecting the three viruses, with a detection limit of 1.0× 10 1 , 1.0× 10 2 , and 1.0 × 10 2 copies for PRSV, PLDMV, and PapMV, respectively. Indeed, the reaction was 100 times more sensitive than the multiplex RT-PCR for PRSV, and 10 times more sensitive than multiplex RT-PCR for PLDMV. Field application of the multiplex real-time RT-PCR demonstrated that some non-symptomatic samples were positive for PLDMV by multiplex real-time RT-PCR but negative by multiplex RT-PCR, whereas some samples were positive for both PRSV and PLDMV by multiplex real-time RT-PCR assay but only positive for PLDMV by multiplex RT-PCR. Th erefore, this multiplex real-time RT-PCR assay provides a more rapid, sensitive and reliable method for simultaneous detection of PRSV, PLDMV, PapMV and their mixed infections in papaya.Keywords: papaya ringspot virus; papaya leaf distortion mosaic virus; papaya mosaic virus; multiplex realtime reverse transcription PCR * Corresponding authors. E-mail: dnaswt@hotmail.com; phone: +86 898 66890687.# Th ese authors contributed equally to this work. Abbreviations: PapMV = papaya mosaic virus; PLDMV = papaya leaf distortion mosaic virus; PRSV = papaya ringspot virus; RT-PCR = reverse transcription PCR; Tm = melting temperature

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Phylogeography and molecular epidemiology of Papaya ringspot virus

Cited by 66 publications

References 56 publications

New species in the papaya ringspot virus cluster: Insights into the evolution of the PRSV lineage

New species in the papaya ringspot virus cluster: Insights into the evolution of the PRSV lineage

Susceptibility assessment of watermelon and pumpkin cultivars to potyvirus in Tocantins

Simultaneous detection of papaya ringspot virus, papaya leaf distortion mosaic virus, and papaya mosaic virus by multiplex real-time reverse transcription PCR

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