Phylogeography and Genetic Diversity of Human Hydatidosis in Bordering the Caspian Sea, Northern Iran by Focusing on Echinococcus granulosus Sensu Stricto Complex

Siyadatpanah, Abolghasem; Daryani, Ahmad; Sarvi, Shahabeddin; Spotin, Adel; Sharif, Mehdi; Dizaji, Reza Esmaeelzadeh; Anvari, Davood; Zeydi, Amir Emami; Kohansal, Mohammad Hasan; Higuita, Nelson Iván Agudelo; Hosseini, Seyed Abdollah; Aryaeipour, Mojgan; Gholami, Shirzad

doi:10.18502/ijph.v49i9.4097

Cited by 2 publications

(1 citation statement)

References 30 publications

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“…Here, it can be seen from the results that, first of all, females are usually numerically more represented than males in ultrasound screening, which increases the possibility of gender selection bias in sampling [ 33 ], which is related to the small sample size. The relationship between sex and echinococcosis has been studied in many studies [ 34 , 35 , 36 ], but inconclusively. Secondly, our study was similar to the result of Khan et al [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Survey and Molecular Characterization of Echinococcus granulosus sensu stricto from Livestock and Humans in the Altai Region of Xinjiang, China

Guo

Zhao

et al. 2023

Pathogens

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Cystic echinococcosis (CE), caused by the metacestode Echinococcus granulosus sensu stricto (s.s.), is an important zoonotic parasite, endemic in the Altai region of China. It is a serious human health risk and causes livestock losses. To evaluate the prevalence, genetic variation, and population structure of CE, 2898 sheep and 703 cattle were examined from October 2019 to mid-February 2020 in the Altai region (Altai, Habahe, Fuhai, and Buerjin). Sheep had an infection rate of 4.52% (131/2898) and cattle had an infection rate of 4.84% (34/703). In total, 180 cyst isolates were obtained, including 131 sheep, 34 cattle, and 15 from CE human patients. The cysts were investigated using mitochondrial cytochrome C oxidase subunit 1 (cox1). Polymerase Chain Reaction (PCR) results showed that, among the two genotypes of E. granulosus s.s., there were 22 different haplotypes (Haps). Phylogenetic analysis and parsimony network indicated that seventeen (77.27%) Haps belonged to the sheep strain (G1 genotype) and five Haps (22.73%) belonged to the buffalo strain (G3 genotype). Hap3 was the most common haplotype (65.00%, 112/180), which belongs to the G1 genotype. Hap18–Hap22 were found in human samples, indicating that sheep and cattle reservoirs of human CE. Molecular diversity indices revealed the high levels of haplotype diversity and relatively low levels of nucleotide diversity. Tajima’s D and Fu’s Fs tests displayed that the Altai population had a significant deviation from neutrality. Based on pairwise fixation index (Fst) values, a low level of genetic differentiation was found between the populations of E. granulosus s.s. isolated from different regions. The present survey findings represent an epidemiological survey of CE in the Altai region where there were two genotypes simultaneously and will provide more information on the genetic structure of E. granulosus s.s. within this region.

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