Phylogeographical Variation of Chloroplast DNA in Cork Oak (Quercus suber)

Lumaret, R.; Tryphon-Dionnet, Mathieu; Michaud, Henri; Sanuy, Aurélie; Ipotesi, Emilie; Born, Céline; Mir, Céline

doi:10.1093/aob/mci237

Cited by 76 publications

(131 citation statements)

References 28 publications

(39 reference statements)

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“…Quercus morisii, instead, clearly exhibited both parental type variants, providing further elements to the ongoing investigations by Toumi and Lumaret (2001), Belahbib et al (2001), and Lumaret et al (2002) on the study of introgressive forms between Q. ilex and Q. suber. It must be noted that this relatively rare hybrid can be found only in presence of both parents.…”

Section: Hybrid Speciesmentioning

confidence: 63%

Utility of ITS sequence data for phylogenetic reconstruction of Italian Quercus spp.

Bellarosa

Simeone

Papini

et al. 2005

Molecular Phylogenetics and Evolution

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Nuclear ribosomal DNA sequences encoding the 5.8S RNA and the Xanking internal transcribed spacers (ITS1 and ITS2) were used to test the phylogenetic relationships within 12 Italian Quercus taxa (Fagaceae). Hypotheses of sequence orthology are tested by detailed inspection of some basic features of oak ITS sequences (i.e., general patterns of conserved domains, thermodynamic stability and predicted conformation of the secondary structure of transcripts) that also allowed more accurate sequence alignment. Analysis of ITS variation supported three monophyletic groups, corresponding to subg. Cerris, Schlerophyllodrys ( D Ilex group sensu Nixon) and Quercus, as proposed by Schwarz [Feddes Rep., Sonderbeih. D,. A derivation of the "Cerris group" from the "Ilex group" is suggested, with Q. cerris sister to the rest of the "Cerris group." Quercus pubescens was found to be sister to the rest of the "Quercus group." The status of hybrispecies of Q. crenata (Q. cerris £ Q. suber) and Q. morisii (Q. ilex £ Q. suber) was evaluated and discussed. Finally, the phylogenetic position of the Italian species in a broader context of the genus is presented. The utility of the ITS marker to assess the molecular systematics of oaks is therefore conWrmed. The importance of Italy as a region with a high degree of diversity at the population and genetic level is discussed. 

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Section: Hybrid Speciesmentioning

confidence: 63%

Utility of ITS sequence data for phylogenetic reconstruction of Italian Quercus spp.

Bellarosa

Simeone

Papini

et al. 2005

Molecular Phylogenetics and Evolution

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“…This finding is supported by the discovery of widespread introgression of ilex-type cpDNA in Q. suber populations (Belahbib et al, 2001;Jiménez et al, 2004;Lumaret et al, 2005), whereas the opposite situation (that is, Q. ilex trees showing suber chlorotypes) is considerably less frequent. However, no evidence of unidirectional gene flow has been found in this study, because we detected a similar number of backcrosses to each species (Figure 2).…”

Section: Detection Of Hybrids In Oaksmentioning

confidence: 79%

“…Despite their deep phylogenetic divergence, clearly supported by internal transcribed spacer, amplified fragment length polymorphisms and isozyme variation (Manos et al, 1999;Toumi and Lumaret, 2001;Bellarosa et al, 2005;Ló pez de Heredia et al, 2007b), hybridization has been inferred on the basis of morphological and molecular markers (Elena-Rosselló et al, 1992;Toumi and Lumaret, 1998;Lumaret et al, 2002;Oliveira et al, 2003;Bellarosa et al, 2005). Furthermore, extensive surveys of chloroplast DNA diversity of both species and of other relatives (such as Q. coccifera) across the whole distribution range have demonstrated widespread cytoplasmic introgression, mainly localized along a northeast-southwest line, from French Catalonia and eastern Iberia to Morocco (reviewed in Lumaret et al, 2005). Interspecific exchanges seem to be limited to introgression of Q. ilex cpDNA and mtDNA into Q. suber, with only very few cases of Q. suber cpDNA introgressing into Q. ilex (Belahbib et al, 2001;Lumaret et al, 2002;Jiménez et al, 2004;Staudt et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Detection of hybrids in nature: application to oaks (Quercus suber and Q. ilex)

et al. 2009

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Powerful and accurate detection of first-generation (F1) hybrids and backcrosses in nature is needed to achieve a better understanding of the function and dynamics of introgression. To document the frequency of ongoing interspecific gene exchange between two Mediterranean evergreen oaks, the cork oak (Quercus suber) and the holm oak (Q. ilex), we analyzed 1487 individuals originating from across the range of the two species using eight microsatellite loci and two Bayesian clustering approaches (implemented in the programs STRUCTURE and NEWHY-BRIDS). Simulated data were used to assess the differences between the two clustering methods and to back up the choice of the threshold value for the posterior probability to discriminate admixed from pure individuals. We found that the use of STRUCTURE resulted in the highest power to detect hybrids, whereas NEWHYBRIDS provided the highest accuracy. Irrespective of the approach, the two species were clearly distinguished as independent genetic entities without any prior information. In contrast with previous reports, we found no evidence for unidirectional introgression. The overall hybridization rate was very low (o2% of introgressed individuals). Only two individuals were identified as F1 hybrids and five as early backcrosses. This work shows that the combined application of the two complementary Bayesian approaches and their systematic validation with simulations, fit for the case at hand, helps gain resolution in the identification of admixed individuals.

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“…Therefore, the preferential direction of hybridization involves pollination of holm oaks by cork oaks, and the common occurrence of ''ilex'' cpDNA haplotypes in cork oaks is indirect evidence of this (Belahbib et al, 2001;Jimé nez et al, 2004;Lumaret et al, 2005;Magri et al, 2007). In contrast, hardly any evidence of ''suber'' cpDNA in holm oaks has been recorded (Belahbib et al, 2001;Collada et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…These hybrids are known to be fertile, as already demonstrated genetically (Oliveira et al, 2003) and previously suggested by cpDNA analysis (Belahbib et al, 2001). The backcross with cork oak would thus generate introgressed progenies which might not be as easily discriminated from the type species, and this possibility in cork production stands is construed as a potential threat to the quality of the product (Natividade, 1936(Natividade, , 1950Belahbib et al, 2001;Jimé nez et al, 2004;Lumaret et al, 2005). Early histological studies of the periderm layer of the trunk in both species and the hybrids confirmed that the intermediate morphology of the cork produced by the hybrids results from the intercalation of ritidome-like outgrowths, similar to those of holm oak (Natividade, 1936).…”

Section: Introductionmentioning

confidence: 99%