Phylogeny, taxon circumscriptions, and character evolution in the core Ptychanthoideae (Lejeuneaceae, Marchantiophyta)

Sukkharak, Phiangphak; Gradstein, S. Robbert; Stech, Michael

doi:10.1002/tax.606006

Cited by 36 publications

(29 citation statements)

References 66 publications

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“…Molecular species delimitation in the R. canescens complex is thus straightforward, in contrast to several other genera of liverworts and mosses analyzed recently, where the presence of para- or polyphyletic species, cryptic speciation, incongruence between molecular markers, or incongruence between molecular and morphological characters complicated species delimitation (e.g. [2], [4], [21]–[24]). …”

Section: Discussionmentioning

confidence: 79%

Molecular Species Delimitation in the Racomitrium canescens Complex (Grimmiaceae) and Implications for DNA Barcoding of Species Complexes in Mosses

et al. 2013

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In bryophytes a morphological species concept is still most commonly employed, but delimitation of closely related species based on morphological characters is often difficult. Here we test morphological species circumscriptions in a species complex of the moss genus Racomitrium, the R. canescens complex, based on variable DNA sequence markers from the plastid (rps4-trnT-trnL region) and nuclear (nrITS) genomes. The extensive morphological variability within the complex has led to different opinions about the number of species and intraspecific taxa to be distinguished. Molecular phylogenetic reconstructions allowed to clearly distinguish all eight currently recognised species of the complex plus a ninth species that was inferred to belong to the complex in earlier molecular analyses. The taxonomic significance of intraspecific sequence variation is discussed. The present molecular data do not support the division of the R. canescens complex into two groups of species (subsections or sections). Most morphological characters, albeit being in part difficult to apply, are reliable for species identification in the R. canescens complex. However, misidentification of collections that were morphologically intermediate between species questioned the suitability of leaf shape as diagnostic character. Four partitions of the molecular markers (rps4-trnT, trnT-trnL, ITS1, ITS2) that could potentially be used for molecular species identification (DNA barcoding) performed almost equally well concerning amplification and sequencing success. Of these, ITS1 provided the highest species discrimination capacity and should be considered as a DNA barcoding marker for mosses, especially in complexes of closely related species. Molecular species identification should be complemented by redefining morphological characters, to develop a set of easy-to-use molecular and non-molecular identification tools for improving biodiversity assessments and ecological research including mosses.

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Section: Discussionmentioning

confidence: 79%

Molecular Species Delimitation in the Racomitrium canescens Complex (Grimmiaceae) and Implications for DNA Barcoding of Species Complexes in Mosses

et al. 2013

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“…Sukkharak et al, 2011;Carter, 2012;Medina et al, 2012;Stech et al, 2013; the present study illustrates the importance of molecular data for clarifying species circumscriptions, resolving taxonomical issues and for the re-evaluation of morphological characters in bryophytes and Dicranum in particular. …”

Section: Discussionmentioning

confidence: 93%

Species delimitations in theDicranum acutifoliumcomplex (Dicranaceae, Bryophyta) using molecular markers

Lang

Tubanova

Stech

2014

Journal of Bryology

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Because of their morphological plasticity and broad geographic distribution, the taxonomy of Dicranum is difficult. The circumscription of the different species included in the Dicranum acutifolium complex is poorly understood and taxonomic confusions are frequent. The present study extends earlier internal transcribed spacer (ITS)-based phylogenetic reconstructions of the D. acutifolium complex by analysing five additional chloroplast markers (trnTrps4, trnL-F, psbA-trnH, rps19-rpl2, rpoB) together with ITS from a larger taxon sample. The phylogenetic analyses delimit Dicranum acutifolium (Lindb. & Arnell) C.E.O.Jensen, D. bardunovii Tubanova & Ignatova, D. brevifolium (Lindb.) Lindb., and D. septentrionale Tubanova & Ignatova, which together form the D. acutifolium complex, and confirm that D. pseudoacutifolium Otnyukova is synonymous with D. flexicaule Brid. Previously Dicranum septentrionale was known from across Russia, but it also occurs in Scandinavia, where it was probably overlooked due to the morphological resemblance to D. brevifolium. The problem of mixed collections for identification is exemplified by the holotype of D. bardunovii, which also contains individuals of the morphologically most similar D. acutifolium, according to the molecular data. Morphometric analyses support the differentiation of the D. acutifolium complex. Furthermore, ordination analyses point to a continuous range of variation among species within the D. acutifolium complex, especially due to the larger variation in D. septentrionale.

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“…Another example of liverwort genus used for chemosystematic studies was genus Thysananthus (43,44). Twenty specimens of four Thysananthus species (T. comosus, T. convolutus, T. retusus, and T. spathulistipus) were collected in Thailand and Malaysia, and the volatile components were analyzed by use of GC/MS.…”

Section: Chemical Relationships Within One Liverwort Genusmentioning

confidence: 99%

Fingerprinting of Secondary Metabolites of Liverworts: Chemosystematic Approach

Ludwiczuk

Asakawa

2014

Journal of AOAC INTERNATIONAL

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The relationship between various types of plants can be predicted based on the similarity in the chemical substances present in them. Compounds that belong to the category of secondary metabolites are of great value in identifying such relationships. Additionally, results from the chemical investigations, together with the other biological or genetic information, can help to understand real relationships among the taxa. Liverworts are small spore-forming plants with simple morphological organization. On the other hand, many liverwort species demonstrate wide geographical distribution and grow under diverse ecological conditions. Because of this, the identification of these plants is especially challenging. One of the outstanding features of the liverworts is their chemistry. They produce a wide array of secondary metabolites, mainly terpenoids and aromatic compounds. Many of these compounds are characterized by unique structures, and some have not been found in any other plants, fungi, or marine organisms. The potential use of chromatographic fingerprinting of the liverworts, as complementary to morphological and genetic information, to resolve the taxonomic problems at the species, genus, and family levels are discussed.

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Phylogeny, taxon circumscriptions, and character evolution in the core Ptychanthoideae (Lejeuneaceae, Marchantiophyta)

Cited by 36 publications

References 66 publications

Molecular Species Delimitation in the Racomitrium canescens Complex (Grimmiaceae) and Implications for DNA Barcoding of Species Complexes in Mosses

Molecular Species Delimitation in the Racomitrium canescens Complex (Grimmiaceae) and Implications for DNA Barcoding of Species Complexes in Mosses

Species delimitations in theDicranum acutifoliumcomplex (Dicranaceae, Bryophyta) using molecular markers

Fingerprinting of Secondary Metabolites of Liverworts: Chemosystematic Approach

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