Phylogeny of ticks (Ixodida) inferred from nuclear ribosomal DNA

Crampton, Andrea; McKay, I.J.; Barker, Stephen C.

doi:10.1016/0020-7519(96)89379-9

Cited by 53 publications

(28 citation statements)

References 8 publications

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“…Within the Acari these genes probably represent the most promising candidates for resolving the deepest relationships within the group. Crampton et al (1996) sequenced 260bp of the 28S rDNA gene and 245bp of the 18S rDNA gene of several species of ticks and confirmed that these regions are both more conserved than 16S rDNA and are suitable for phylogenies at the family and subfamily levels. Since these sequences were rather short, Black et al (1997) sequenced the entire 18S gene (1846bp) for a smaller number of taxa.…”

Section: Nuclear Ribosomal Genesmentioning

confidence: 99%

Molecular markers for the phylogenetics of mites and ticks

Cruickshank

2002

Systematic and Applied Acarology

159

119

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Molecular methods are becoming increasingly important in systematic acarology. In this review I describe the properties of the ideal molecular marker and compare these with genes that have been used for phylogenetic studies of mites and ticks. The second internal transcribed spacer of the nuclear ribosomal gene cluster (ITS2) and the mitochondrial protein-coding gene cytochrome oxidase I (COI) together provide a powerful tool for phylogenetics at low taxonomic levels. The nuclear ribosomal genes 18S and 28S rDNA are equally powerful tool for phylogenetics at the deepest levels within the Acari. What appear to be lacking are markers that are useful at intermediate levels. The mitochondrial ribosomal genes 12S and 16S rDNA have not lived up to their initial promise. I suggest some nuclear protein-coding genes that may be suitable alternatives. Methods for collection and storage of mites for molecular work, DNA extraction and phylogenetic analysis are also briefly discussed.

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Section: Nuclear Ribosomal Genesmentioning

confidence: 99%

Molecular markers for the phylogenetics of mites and ticks

Cruickshank

2002

Systematic and Applied Acarology

159

119

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“…There is some controversy regarding the phylogenetic positions of these subfamilies and genera (NAVA et al, 2009). Some phylogenetic studies on ixodids have inferred that there is a close relationship between Rhipicephalinae and Haemaphysalinae, such that Amblyomminae had an underlying position in relation to them (CRAMPTON et al, 1996;BLACK et al, 1997;MANGOLD et al, 1998). However, in another analysis based in 16s rDNA, Amblyomminae and Haemaphysalinae were placed on a common branch, but Amblyomminae was paraphyletic, with the species A. americanum and A. sculptum (cited as A. cajennense) close to Rhipicephalinae, and A. variegatum and Amblyomma hebraeum Koch, 1844, correlated with Haemaphysalinae (BLACK & PIESMAN, 1994).…”

Section: Phylogenetic Inferencesmentioning

confidence: 99%

Gut transcriptome analysis on females of Ornithodoros mimon (Acari: Argasidae) and phylogenetic inference of ticks

Landulfo

Patané

Silva

et al. 2017

Rev. Bras. Parasitol. Vet.

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Ornithodoros mimon is an argasid tick that parasitizes bats, birds and opossums and is also harmful to humans. Knowledge of the transcripts present in the tick gut helps in understanding the role of vital molecules in the digestion process and parasite-host relationship, while also providing information about the evolution of arthropod hematophagy. Thus, the present study aimed to know and ascertain the main molecules expressed in the gut of argasid after their blood meal, through analysis on the gut transcriptome of engorged females of O. mimon using 454-based RNA sequencing. The gut transcriptome analysis reveals several transcripts associated with hemoglobin digestion, such as serine, cysteine, aspartic proteases and metalloenzymes. The phylogenetic analysis on the peptidases confirmed that most of them are clustered with other tick genes. We recorded the presence a cathepsin O peptidase-coding transcript in ticks. The topology of the phylogenetic inferences, based on transcripts of inferred families of homologues, was similar to that of previous reports based on mitochondrial genome and nuclear rRNA sequences. We deposited 2,213 sequence of O. mimon to the public databases. Our findings may help towards better understanding of important argasid metabolic processes, such as digestion, nutrition and immunity.Keywords: Argasidae, transcriptome, gut, phylogeny, Ornithodoros mimon. ResumoOrnithodoros mimon é um carrapato argasídeo parasita de morcegos, aves e marsupiais, além de ser bastante agressivo aos humanos. O conhecimento dos transcritos presentes no intestino dos carrapatos auxilia no entendimento do papel de moléculas vitais no processo de digestão e na relação parasito-hospedeiro, além de fornecer também informações sobre a evolução dos artrópodes hematófagos. Desta maneira, o presente estudo teve como objetivo conhecer e identificar as principais moléculas expressas no intestino de uma espécie de carrapato argasídeo após o repasto sanguíneo, através de uma análise transcritômica descritiva do intestino de fêmeas ingurgitadas de O. mimon, utilizando um sequenciamento de RNA de nova geração da plataforma 454. Além de inferir a relação filogenética de carrapatos através de um conjunto de dados transcritômicos. O transcriptoma do intestino revelou diversos transcritos associados com a digestão da hemoglobina, como proteinases das classes serino, cisteína, aspártica e metalo. Registramos a presença de um transcrito de uma cisteína peptidase do tipo catepsina O em carrapatos. A inferência filogenética baseada em conjunto de dados transcritos homólogos tem uma resolução topológica similar a de outros conjuntos de dados moleculares. Foram depositados no banco de dados gênico público 2213 transcritos de O. mimon. Os achados obtidos no presente estudo podem contribuir para compreensão dos importantes processos, como digestão, nutrição e imunidade dos carrapatos da família Argasidae, além de fornecer informações sobre a filogenia da ordem Ixodida.

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“…This cDNA was used as template for the polymerase chain reaction (PCR). Molecular confirmation of tick species was done by PCR amplification of mitochondrial 16S rRNA gene and V4 region of 18S rRNA gene (Crampton et al, 1996;Kumar et al, 2011). The primer sequences for PCR amplification are mentioned in table 1.…”

Section: Methodsmentioning

confidence: 99%