Phylogeny of lymphocyte heterogeneity: Identification and separation of functionally distinct subpopulations of channel catfish lymphocytes with monoclonal antibodies1

Miller, Norman W.; Bly, Jan E.; Ginkel, F. van; Ellsaesser, Catherine F.; Clem, L. William

doi:10.1016/0145-305x(87)90061-9

Cited by 125 publications

(45 citation statements)

References 15 publications

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“…117,118 Soon following, monoclonal antibodies were developed which reacted with T lymphocytes (although in some cases cross-reacting with other cell types) which formed the basis for flow cytometry-based investigations. 108,119,120 The unequivocal confirmation of T cells in channel catfish can be ascribed to the cloning of TCRa and TCRb genes and the establishment of clonal T cell lines. [121][122][123][124] Indeed, much of what is known regarding catfish T cells arose from the development of these T cell lines, which were produced by limiting dilutions of peripheral blood lymphocytes (PBL) isolated from a single fish that had been immunized repeatedly with the catfish autonomous B cell line 3B11.…”

Section: Ab T Cellsmentioning

confidence: 99%

“…[104][105][106] In the late eighties, new methodologies were established to characterize and identify B cells and Ab responses in the channel catfish. 107,108 The development of different catfish IgM monoclonal Abs would allow for more quantitative monitoring of humoral immune responses. Most of these studies have used fish pathogens as models for activating adaptive immune responses in the channel catfish.…”

Section: Adaptive Immunity At Catfish Mucosal Barriersmentioning

confidence: 99%

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Physiology and immunology of mucosal barriers in catfish (Ictalurus spp.)

et al. 2015

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The mucosal barriers of catfish (Ictalurus spp) constitute the first line of defense against pathogen invasion while simultaneously carrying out a diverse array of other critical physiological processes, includ\ing nutrient adsorption, osmoregulation, waste excretion, and environmental sensing. Catfish depend more heavily on mucosal barriers than their terrestrial counterparts as they are continuously interacting with the aquatic microbiota. Our understanding of these barriers, while growing, is still limited relative to that of mammalian model systems. Nevertheless, a combination of molecular and cellular studies in catfish over the last few decades, and particularly within the last few years, has helped to elucidate many of the primary actors and pathways critical to their mucosal health. Here we describe aspects of innate and adaptive immune responses in the primary mucosal tissues (skin, gill, and intestine) of catfish, focusing on mucus-driven responses, pathogen recognition, soluble mediators, and immunoglobulin and T-cell derived immunity. Modulation of mucosal barriers will be critical moving forward for crafting better diets, improving vaccine delivery, enhancing water quality, and ensuring sustainable production practices in catfish.

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Section: Ab T Cellsmentioning

confidence: 99%

Section: Adaptive Immunity At Catfish Mucosal Barriersmentioning

confidence: 99%

Physiology and immunology of mucosal barriers in catfish (Ictalurus spp.)

et al. 2015

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“…The CC41 mAb did not react in Western blot or in ELISA. Other mAbs used in this study were as follows: mouse anti-catfish IgM 9E1 (IgG1, k) reacts with catfish Igm chains (44) and mouse anti-catfish IgL 3F12 (IgG1, k) reacts with the IgL F isotype (45); mouse anti-catfish IgL 11A2 (IgG2b, k) reacts with IgL G isotype (45); and as an isotype control, mouse anti-trout IgM 1.14 (IgG1, k), which reacts with trout Igm chains (46).…”

Section: Monoclonal Absmentioning

confidence: 99%

A Leukocyte Immune-Type Receptor Subset Is a Marker of Antiviral Cytotoxic Cells in Channel Catfish, Ictalurus punctatus

Taylor

Moulana

Stuge

et al. 2016

The Journal of Immunology

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Channel catfish, Ictalurus punctatus, leukocyte immune type receptors (LITRs) represent a multigene family that encodes Ig superfamily proteins that mediate activating or inhibitory signaling. In this study, we demonstrate the use of mAb CC41 to monitor viral cytotoxic responses in catfish and determine that CC41 binds to a subset of LITRs on the surface of catfish clonal CTLs. Homozygous gynogenetic catfish were immunized with channel catfish virus (CCV)–infected MHC-matched clonal T cells (G14D-CCV), and PBL were collected at various times after immunization for flow cytometric analyses. The percentage of CC41+ cells was significantly increased 5 d after primary immunization with G14D-CCV and at 3 d after a booster immunization as compared with control fish only injected with G14D. Moreover, CC41+ cells magnetically isolated from the PBL specifically killed CCV-infected targets as measured by 51Cr release assays and expressed messages for CD3γδ, perforin, and at least one of the CD4-like receptors as analyzed by RNA flow cytometry. When MLC effector cells derived from a G14D-CCV–immunized fish were preincubated with CC41 mAb, killing of G14D-CCV targets was reduced by ∼40%, suggesting that at least some LITRs have a role in target cell recognition and/or cytotoxicity. The availability of a LITR-specific mAb has allowed, to our knowledge for the first time, functional characterization of LITRs in an autologous system. In addition, the identification of an LITR subset as a cytotoxic cell marker will allow for more effective monitoring of catfish immune responses to pathogens.

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“…42TA is a macrophage cell line [44]. Catfish PBL were isolated from heparinized blood by centrifugation on a cushion of Ficoll-Hypaque (Lymphoprep, Accurate Chemical Corp. Westbury, NY) as described previously [45]. The 1G8 and 3B11 time course study was performed by initiating cultures with approximately 2 × 10 5 /ml 1G8 and/or 3B11 B cells on day 0 and harvesting cells on days 1-8 for RNA.…”

Section: Animals and Clonal Cell Linesmentioning

confidence: 99%

“…Briefly, catfish PBL were isolated from heparinized blood as above and 2 × 10 8 PBL were incubated with 1ml of monoclonal antibody (mAb) anti-catfish IgM 9E1 [45] supernatant for 30 min at 4°C and washed with RPMI. Cells were then resuspended in 240 μl of RPMI supplemented with 2 mM EDTA and 0.5% BSA.…”

Section: Magnetic Cell Sorting Rna Preparation and Reverse Transcripmentioning

confidence: 99%

B cell receptor accessory molecules in the channel catfish, Ictalurus punctatus

Sahoo

Edholm

Stafford

et al. 2008

Developmental & Comparative Immunology

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B cell receptor (BCR) accessory molecules CD79a and CD79b homologs were identified in the channel catfish, Ictalurus punctatus. Both are found as single copy genes that encode proteins containing a signal peptide, an extracellular Ig domain, a transmembrane region and a cytoplasmic tail containing an immunoreceptor tyrosine kinase activation motif (ITAM). IpCD79a and IpCD79b transcripts correlate well with IgM message expression. They are highly expressed in peripheral blood leukocytes (PBL) enriched in membrane (m) IgM + cells and catfish clonal B cell lines, but not in catfish clonal T cells, indicating that IpCD79a and IpCD79b expression is B cell restricted. Studies using catfish clonal B cells (3B11) transfected with constructs encoding epitope-tagged IpCD79a and IpCD79b revealed that IpCD79a was expressed as a 45 kDa protein and IpCD79b was expressed as a 32 kDa protein. Furthermore, co-immunoprecipitations of epitope-tagged CD79 proteins demonstrate that these molecules are non-covalently associated with mIgM. These data correlate with some of the previous immunoprecipitation data demonstrating that catfish mIgM associates with proteins of 45 and 32 kDa.

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Phylogeny of lymphocyte heterogeneity: Identification and separation of functionally distinct subpopulations of channel catfish lymphocytes with monoclonal antibodies1

Cited by 125 publications

References 15 publications

Physiology and immunology of mucosal barriers in catfish (Ictalurus spp.)

Physiology and immunology of mucosal barriers in catfish (Ictalurus spp.)

A Leukocyte Immune-Type Receptor Subset Is a Marker of Antiviral Cytotoxic Cells in Channel Catfish, Ictalurus punctatus

B cell receptor accessory molecules in the channel catfish, Ictalurus punctatus

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