Phylogeny of immunoglobulin structure and function-X. Humoral immunoglobulins of the sheepshead, Archosargus probatocephalus

Characterisation of monoclonal antibodies specific for sea bass (Dicentrarchus labraxL.) IgM indicates the existence of B cell subpopulations

“…Additionally, Lobb & Clem (1983) showed that the high molecular weight IgM from catfish could be dissociated, in the presence of denaturing agents, into eight distinct subunits formed by covalently-linked combinations of H-L chain pairs (' halfmers '). Di#erences in secretory and serum Ig were also studied in sheepshead (Lobb & Clem, 1981), rainbow trout (St Louis-Cormier et al, 1984), catfish (Lobb, 1987) and carp . Whether these di#erent molecules represent distinct isotypes of IgM and/or they have a di#erent physiological significance remains to be investigated.…”

Isolation and partial characterization of serum immunoglobulins from sea bass (Dicentrarchus labraxL.) and gilthead sea bream (Sparus aurataL.)

“…As for the family Sparidae, few complete studies are available in the literature (Lobb & Clem, 1981a). Thus far, most teleostean serum immunoglobulins have been characterized as tetrameric IgM-like macroglobulins with molecular weights between 600 000 Da and 800 000 Da (Acton et al, 1971;Litman 1976;Dorson, 1981).…”

Isolation and characterization of immunoglobulin M of Asian sea bass, Lates calcarifer and its level in serum

“…The mean value of the IgM concentration in the sera of ten healthy sea bass was 5.4 ±1.8 mg ml -1 , which is quite similar to the serum IgM concentration of cod, 5.63 mg ml -1 [39] and 6.48 mg ml -1 in turbot [34]. However, there is a lot of variation in serum Ig concentration reported in other fish species such as sheepshead-3.07 mg ml -1 [36], rainbow trout-3.3 mg ml -1 [40], paddlefish-17.2 mg ml -1 [41], yellowtail-0.96 mg ml -1 [42] and Atlantic salmon-0.8 mg ml -1 [4]. This variation in IgM levels may be due to the difference of age between fishes or due to different environmental factors such as different conditions where the fishes were reared.…”

“…Upon treatment with SDS, these Ig molecules were dissociated into such structures possessing only the covalent interactions and were unable for further dissociation and thus generated the dimer and monomer. Such variability among H 2 L 2 subunit linkages has been reported in flounder [35], sheepshead [36], perch [7] and salmon [37]. All of these teleost species appear to be unique in their assembly of IgM and these types of molecules are now termed as redox form [38].…”