Phylogeny of Chaetothyriaceae in northern Thailand including three new species

Chomnunti, Putarak; Ko, Thida Win Ko; Chukeatirote, Ekachai; Hyde, Kevin D.; Cai, Lei; Jones, E. B. Gareth; Kodsueb, Rampai; Hassan, Bahkali Ali; Chen, Hang

doi:10.3852/11-066

Cited by 45 publications

(45 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The likely reason for this low identification rate is the absence of ITS-region sequence data for these sooty mould families. Recent molecular characterisation of sooty mould fungi within the family Capnodiaceae and related Chaetothyriaceae suggests that both these families contain unrelated taxa that belong to different classes but cannot be differentiated on the basis of morphology and growth habit [32], [33]. Sooty mould families from Orders Chaetothyriales ( Coccodiniaceae ) and Pleosporales ( Triposporipsidae ) [34] were not identified although other families from these orders were represented in this study.…”

Section: Discussionmentioning

confidence: 83%

Diverse Honeydew-Consuming Fungal Communities Associated with Scale Insects

et al. 2013

View full text Add to dashboard Cite

Sooty mould fungi are ubiquitous, abundant consumers of insect-honeydew that have been little-studied. They form a complex of unrelated fungi that coexist and compete for honeydew, which is a chemically complex resource. In this study, we used scanning electron microscopy in combination with T-RFLP community profiling and ITS-based tag-pyrosequencing to extensively describe the sooty mould community associated with the honeydews of two ecologically important New Zealand coelostomidiid scale insects, Coelostomidia wairoensis and Ultracoelostoma brittini. We tested the influence of host plant on the community composition of associated sooty moulds, and undertook limited analyses to examine the influence of scale insect species and geographic location. We report here a previously unknown degree of fungal diversity present in this complex, with pyrosequencing detecting on average 243 operational taxonomic units across the different sooty mould samples. In contrast, T-RFLP detected only a total of 24 different “species” (unique peaks). Nevertheless, both techniques identified similar patterns of diversity suggesting that either method is appropriate for community profiling. The composition of the microbial community associated with individual scale insect species varied although the differences may in part reflect variation in host preference and site. Scanning electron microscopy visualised an intertwined mass of fungal hyphae and fruiting bodies in near-intact physical condition, but was unable to distinguish between the different fungal communities on a morphological level, highlighting the need for molecular research. The substantial diversity revealed for the first time by pyrosequencing and our inability to identify two-thirds of the diversity to further than the fungal division highlights the significant gap in our knowledge of these fungal groups. This study provides a first extensive look at the community diversity of the fungal community closely associated with the keystone insect-honeydew systems of New Zealand’s native forests and suggests there is much to learn about sooty mould communities.

show abstract

Section: Discussionmentioning

confidence: 83%

Diverse Honeydew-Consuming Fungal Communities Associated with Scale Insects

et al. 2013

View full text Add to dashboard Cite

show abstract

“…In the general phylogeny of Fig. 1, numerous members are epiphytic on plants, occurring as sooty moulds (Chomnunti et al. 2012b) or as rock-inhabiting fungi (Isola et al.…”

Section: Discussionmentioning

confidence: 99%

Exploring the genomic diversity of black yeasts and relatives (Chaetothyriales,Ascomycota)

Teixeira¹,

Moreno²,

Stielow³

et al. 2017

Studies in Mycology

155

217

View full text Add to dashboard Cite

The order Chaetothyriales (Pezizomycotina, Ascomycetes) harbours obligatorily melanised fungi and includes numerous etiologic agents of chromoblastomycosis, phaeohyphomycosis and other diseases of vertebrate hosts. Diseases range from mild cutaneous to fatal cerebral or disseminated infections and affect humans and cold-blooded animals globally. In addition, Chaetothyriales comprise species with aquatic, rock-inhabiting, ant-associated, and mycoparasitic life-styles, as well as species that tolerate toxic compounds, suggesting a high degree of versatile extremotolerance. To understand their biology and divergent niche occupation, we sequenced and annotated a set of 23 genomes of main the human opportunists within the Chaetothyriales as well as related environmental species. Our analyses included fungi with diverse life-styles, namely opportunistic pathogens and closely related saprobes, to identify genomic adaptations related to pathogenesis. Furthermore, ecological preferences of Chaetothyriales were analysed, in conjuncture with the order-level phylogeny based on conserved ribosomal genes. General characteristics, phylogenomic relationships, transposable elements, sex-related genes, protein family evolution, genes related to protein degradation (MEROPS), carbohydrate-active enzymes (CAZymes), melanin synthesis and secondary metabolism were investigated and compared between species. Genome assemblies varied from 25.81 Mb (Capronia coronata) to 43.03 Mb (Cladophialophora immunda). The bantiana-clade contained the highest number of predicted genes (12 817 on average) as well as larger genomes. We found a low content of mobile elements, with DNA transposons from Tc1/Mariner superfamily being the most abundant across analysed species. Additionally, we identified a reduction of carbohydrate degrading enzymes, specifically many of the Glycosyl Hydrolase (GH) class, while most of the Pectin Lyase (PL) genes were lost in etiological agents of chromoblastomycosis and phaeohyphomycosis. An expansion was found in protein degrading peptidase enzyme families S12 (serine-type D-Ala-D-Ala carboxypeptidases) and M38 (isoaspartyl dipeptidases). Based on genomic information, a wide range of abilities of melanin biosynthesis was revealed; genes related to metabolically distinct DHN, DOPA and pyomelanin pathways were identified. The MAT (MAting Type) locus and other sex-related genes were recognized in all 23 black fungi. Members of the asexual genera Fonsecaea and Cladophialophora appear to be heterothallic with a single copy of either MAT-1-1 or MAT-1-2 in each individual. All Capronia species are homothallic as both MAT1-1 and MAT1-2 genes were found in each single genome. The genomic synteny of the MAT-locus flanking genes (SLA2-APN2-COX13) is not conserved in black fungi as is commonly observed in Eurotiomycetes, indicating a unique genomic context for MAT in those species. The heterokaryon (het) genes expansion associated with the low selective pressure at the MAT-locus suggests that a parasexual cycle may play an important r...

show abstract

“…Fungi of the order Chaetothyriales (Ascomycetes), also referred to as 'black yeasts and relatives', are mostly known from a range of oligotrophic or extreme environments, such as bare rocks, plant surfaces, indoor surfaces of buildings and substrates contaminated with aromatic hydrocarbons [1][2][3][4]. Some Chaetothyriales are also opportunistic human pathogens causing various diseases ranging from skin to neurotropic infections [5].…”

Section: Introductionmentioning

confidence: 99%

A phylogenetic perspective on the association between ants (Hymenoptera: Formicidae) and black yeasts (Ascomycota: Chaetothyriales)

et al. 2017

View full text Add to dashboard Cite

The frequency and the geographical extent of symbiotic associations between ants and fungi of the order Chaetothyriales have been highlighted only recently. Using a phylogenetic approach based on seven molecular markers, we showed that ant-associated Chaetothyriales are scattered through the phylogeny of this order. There was no clustering according to geographical origin or to the taxonomy of the ant host. However, strains tended to be clustered according to the type of association with ants: strains from ant-made carton and strains from plant cavities occupied by ants (‘domatia’) rarely clustered together. Defining molecular operational taxonomic units (MOTUs) with an internal transcribed spacer sequence similarity cut-off of 99% revealed that a single MOTU could be composed of strains collected from various ant species and from several continents. Some ant-associated MOTUs also contained strains isolated from habitats other than ant-associated structures. Altogether, our results suggest that the degree of specialization of the interactions between ants and their fungal partners is highly variable. A better knowledge of the ecology of these interactions and a more comprehensive sampling of the fungal order are needed to elucidate the evolutionary history of mutualistic symbioses between ants and Chaetothyriales.

show abstract

Phylogeny of Chaetothyriaceae in northern Thailand including three new species

Cited by 45 publications

References 34 publications

Diverse Honeydew-Consuming Fungal Communities Associated with Scale Insects

Diverse Honeydew-Consuming Fungal Communities Associated with Scale Insects

Exploring the genomic diversity of black yeasts and relatives (Chaetothyriales,Ascomycota)

A phylogenetic perspective on the association between ants (Hymenoptera: Formicidae) and black yeasts (Ascomycota: Chaetothyriales)

Contact Info

Product

Resources

About