2018
DOI: 10.3114/fuse.2018.01.04
|View full text |Cite
|
Sign up to set email alerts
|

Phylogeny and taxonomy of the genus Tubakia s. lat.

Abstract: The genus Tubakia is revised on the basis of morphological and phylogenetic data. The phylogenetic affinity of Tubakia to the family Melanconiellaceae (Diaporthales) was recently postulated, but new analyses based on sequences retrieved from material of the type species of Tubakia, T. dryina, support a family of its own, viz. Tubakiaceae fam. nov. Our phylogenetic analyses revealed the heterogeneity of Tubakia s. lat. which is divided into several genera, viz., Tubakia s. str., Apiognomonioides gen. nov. (type… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

3
93
0
1

Year Published

2018
2018
2022
2022

Publication Types

Select...
8

Relationship

2
6

Authors

Journals

citations
Cited by 48 publications
(97 citation statements)
references
References 35 publications
3
93
0
1
Order By: Relevance
“…Other loci were sequenced for various species or genera using primers and conditions specific for those groups of fungi (Table 1). Amplification of the partial DNA-directed RNA polymerase II second largest subunit gene (rpb2), the partial translation elongation factor 1-alpha gene (tef1) and the partial beta-tubulin gene (tub2) followed Braun et al (2018), while the amplification of the partial actin gene (act), the partial calmodulin gene (cmdA), the partial glyceraldehyde-3-phosphate dehydrogenase gene (gapdh) and the partial histone H3 gene (his3) followed Videira et al (2016). The resulting fragments were sequenced in both directions using the respective PCR primers and the BigDye Terminator Cycle Sequencing Kit v. 3.1 (Applied Biosystems Life Technologies, Carlsbad, CA, USA); DNA sequencing amplicons were purified through Sephadex G-50 Superfine columns (Sigma-Aldrich, St. Louis, MO) in MultiScreen HV plates (Millipore, Billerica, MA).…”
Section: Dna Extraction Amplification (Pcr) and Phylogenymentioning
confidence: 99%
See 2 more Smart Citations
“…Other loci were sequenced for various species or genera using primers and conditions specific for those groups of fungi (Table 1). Amplification of the partial DNA-directed RNA polymerase II second largest subunit gene (rpb2), the partial translation elongation factor 1-alpha gene (tef1) and the partial beta-tubulin gene (tub2) followed Braun et al (2018), while the amplification of the partial actin gene (act), the partial calmodulin gene (cmdA), the partial glyceraldehyde-3-phosphate dehydrogenase gene (gapdh) and the partial histone H3 gene (his3) followed Videira et al (2016). The resulting fragments were sequenced in both directions using the respective PCR primers and the BigDye Terminator Cycle Sequencing Kit v. 3.1 (Applied Biosystems Life Technologies, Carlsbad, CA, USA); DNA sequencing amplicons were purified through Sephadex G-50 Superfine columns (Sigma-Aldrich, St. Louis, MO) in MultiScreen HV plates (Millipore, Billerica, MA).…”
Section: Dna Extraction Amplification (Pcr) and Phylogenymentioning
confidence: 99%
“…The results are provided as part of the species notes or as selected phylogenetic trees. Phylogenetic trees were generated using Bayesian analyses performed with MrBayes v. 3.2.6 (Ronquist et al 2012) for the overview trees and Maximum Parsimony analyses performed with PAUP v. 4.0b10 (Swofford 2003) as explained in Braun et al (2018) for the genus and species trees. All resulting trees were printed with Geneious v. 11.0.3 (http://www.geneious.com, Kearse et al 2012) and the layout of the trees was done in Adobe Illustrator v. CC 2017.…”
Section: Dna Extraction Amplification (Pcr) and Phylogenymentioning
confidence: 99%
See 1 more Smart Citation
“…Statistical support for the branches was evaluated using a bootstrap analysis (BS) of 1000 replicates. MP analyses were carried out using PAUP v.4.0b10 (Swofford 2003) as described by Braun et al (2018). Statistical support for the branches was evaluated using a bootstrap analysis (PBS) of 1000 replicates.…”
Section: Sequence Alignment and Molecular Phylogenetic Analysismentioning
confidence: 99%
“…Four partial nuclear genes were subjected to PCR amplification and sequencing. These included the 28S nrRNA gene (LSU), internal transcribed spacer regions and intervening 5.8S nrRNA gene (ITS) of the nrDNA operon, DNA-directed RNA polymerase II second largest subunit gene (rpb2), beta-tubulin (tub2) and translation elongation factor 1-alpha (tef1) using the primers and conditions explained in Braun et al (2018). The resulting fragments were sequenced in both directions using the respective PCR primers and the BigDye Terminator Cycle Sequencing Kit v. 3.1 (Applied Biosystems Life Technologies, Carlsbad, CA, USA).…”
Section: Dna Isolation Amplification and Analysesmentioning
confidence: 99%