Phylogeny and Systematics of the Genus Tolypocladium (Ophiocordycipitaceae, Hypocreales)

Dong, Quanying; Wang, Yao; Wang, Zhiqin; Liu, Yanfang; Yu, Hong

doi:10.3390/jof8111158

Cited by 4 publications

(1 citation statement)

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“…Wash and peel the potatoes, cut them into thin slices, add water 1000 mL, and boil for about 20 min; then mix, heat, divide into conical flasks, kill bacteria at 121 °C for 30 min, cool to 50 °C, add 0.1 g streptomycin and 0.05 g tetracycline per liter to inhibit the growth of bacteria. Plates were poured at approximately 10 mL per flask (Dong et al 2022). Second, the seed liquid was cultured by low-temperature preservation of the tube slope strain inoculated on the PDA solid medium for 10 days at 25 °C, and a large number of colonies covered the surface of the medium.…”

Section: Sample Collection and Culture Preparationmentioning

confidence: 99%

Comprehensive analysis of metabolites in the mycelium of Cordyceps fumosorosea cultured on Periplaneta americana

Khan,

Dong-Hai,

Zhou

et al. 2024

Ann Microbiol

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Background Cordyceps fumosorosea is one of the common species within the Cordyceps genus, which are cultured on Periplaneta americana. This study aimed to determine the composition of bioactive compounds, including β-glucans, polysaccharides, cordycepic acid, flavonoids, ergosterol, and nitrogenous compounds (specifically nucleosides: adenosine, guanosine, adenine, and hypoxanthine), present in the mixture and mycelium at various time incubation. Methods Different bioactive compounds, including β-1,3-glucan, polysaccharides, cordycepic acid, flavonoids, ergosterol, and nitrogenous compounds (specifically nucleosides: adenosine, guanosine, adenine, and hypoxanthine) are detected from C. fumosorosea which cultured on P. americana by UV and HPLC. Results Mycelia of C. fumosorosea were cultivated in P. americana (medium). The highest total β-1,3-glucan content was observed in the mixture (C. fumosorosea + P. americana) after 25 days (69.21 ± 0.07 mg/g) and in the mycelium after 25 days (56.32 ± 0.39 mg/g) using different solvents. The highest β-1,3-glucan content was attained at specific time incubation in other solvents. The content of cordycepic acid peaked at 52.28 ± 0.11 mg/g in the mixture after 25 days and at 46.96 ± 0.13 mg/g in the mycelium after 25 days. The polysaccharide content reached its highest level in the mixture after 20 days (16.68 ± 0.38 mg/g) and in the mycelium after 20 days (14.85 ± 0.10 mg/g). The peak flavonoid content was observed in the mixture after 25 days (4.65 ± 0.24 mg/g) and in the mycelium after 25 days (4.04 ± 0.07 mg/g). Nucleosides, including adenine, adenosine, hypoxanthine, and guanosine, exhibited their highest levels after 25 days in the mixture and mycelium. Ergosterol content peaks at 25 days (2.25 ± 0.04 mg/g). Conclusion Cordyceps fumosorosea mixture and time incubation of 20 and 25 days are optimal for detecting a diverse array of bioactive compounds, including β-1,3-glucan, polysaccharides, cordycepic acid, flavonoids, ergosterol, and nucleosides.

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Section: Sample Collection and Culture Preparationmentioning

confidence: 99%