Phylogeny and polycyclic aromatic hydrocarbons degradation potential of bacteria isolated from crude oil-contaminated site

Abstract

This study employed the use of 16S rRNA gene sequence analysis to identify three of four native bacterial strains isolated from crude oil-contaminated site in Poza Rica, Veracruz, Mexico. The identified bacteria were Ochrobactrum intermedium, Pandoraea pnomenusa and Ochrobactrum sp., but SA2-09 strain was not identified. The ability of the isolates to degrade polycyclic aromatic hydrocarbons (PAHs) was evaluated at 31.61 and 54.52 mg/kg PAHs in soil, when used as crude oil in soil microcosm during 80 days of incubation at 30°C. The results demonstrated that O. intermedium biodegraded many PAHs, including the high molecular weight (HMW) PAHs fluoranthene (100% equivalent 0.24 mg/kg), benzo [b] fluoranthene (81.8% equal 0.18 mg/kg), Benzo[a]pyrene (87.0%, 0.20 mg/kg) and Benzo[g,h,i]perylene (52.7%, 0.39 mg/kg). P. pnomenusa had a degradation profile of HMW PAHs, which was similar to O. intermedium, while Ochrobactrum sp. and the strain SA-09 exhibited lower degradation rates of HMW.

Evaluation of Amycolaptosis sp. Ver12 as a potential degrader of benzo(b)fluoranthene

“…SA‐04 degraded BbF from an initial concentration of 0⋅22 mg kg −1 by 81, 50 and 60%, respectively, after 80 days of incubation (Tirado‐Torres et al . 2017). In another study, Achromobacter xylosoxidans degraded 20% of BbF from 1 mg kg −1 after 15 days of incubation, and with the addition of glucose (0⋅25%) the removal was of 98%.…”

Comparative Genomics of Pandoraea, a Genus Enriched in Xenobiotic Biodegradation and Metabolism

“…Because many Pandoraea strains participate in the biodegradation of recalcitrant xenobiotics (Uhlik et al, 2012; Pushiri et al, 2013; Shi et al, 2013; Wang et al, 2015; Crofts et al, 2017; de Paula et al, 2017; Sarkar et al, 2017; Tirado-Torres et al, 2017; Kumar et al, 2018b; Yang et al, 2018; Liu et al, 2019; Wu et al, 2019), we specifically looked at the orthogroups in the KEGG pathway Xenobiotics biodegradation and metabolism (Figure 7). Most orthogroups in this pathway were present in multiple species ( n = 28) and some were even present in the core Pandoraea genome ( n = 6).…”

“…The latter suggests they may be important contributors to soil formation, soil fertility and retention, and cycling of elements necessary for plant growth (Sahin, 2003). These free-living Pandoraea bacteria are often enriched in polluted soils and participate in the biodegradation of complex organic substances including lignin (Shi et al, 2013; Kumar et al, 2018b; Liu et al, 2019), biodiesel and petroleum by-products (de Paula et al, 2017; Sarkar et al, 2017; Tirado-Torres et al, 2017), p -xylene (Wang et al, 2015), δ-hexachlorocyclohexane (Pushiri et al, 2013), di- n -butyl phthalate (Yang et al, 2018), biphenyl, benzoate and naphthalene (Uhlik et al, 2012), and tetracycline (Wu et al, 2019) and β-lactam antibiotics (Crofts et al, 2017). A particularly well-documented Pandoraea strain, i.e., JB1 T (LMG 31106 T ), was isolated in the 1980s from garden soil (Parsons et al, 1988) and was able to use biphenyl, 2-, 3- and 4-chloro-biphenyl, m -toluate, p -toluate naphthalene, m -hydroxybenzoate and diphenylmethane (Springael et al, 1996).…”