Phylogeny and Phylogeography of Rhizobial Symbionts Nodulating Legumes of the Tribe Genisteae

Stępkowski, Tomasz; Banasiewicz, Joanna; Granada, Camille Eichelberger; Andrews, Mitchell; Passaglia, Luciane Maria Pereira

doi:10.3390/genes9030163

Cited by 68 publications

(61 citation statements)

References 168 publications

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“…None of our isolates was affiliated to Bradyrhizobium, unlike in previous studies on C. spinosa in the northeast of Algeria [6] and C. spinosa and C. infesta in different areas of Sicily [5]. Several authors have demonstrated that Bradyrhizobium predominates among rhizobia nodulating Genisteae [47][48][49][50]. However, in some other studies, Genistoid plants, such as Genista saharae, Retama raetam or Retama sphaerocarpa, were nodulated by Sinorhizobium (formerly Ensifer), Rhizobium, Mesorhizobium and Phyllobacterium [39,51,52].…”

Section: Rhizobia Isolated From C Villosa Nodules In Tunisian Arid Scontrasting

confidence: 79%

The ACC-Deaminase Producing Bacterium Variovorax sp. CT7.15 as a Tool for Improving Calicotome villosa Nodulation and Growth in Arid Regions of Tunisia

et al. 2020

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Calicotome villosa is a spontaneous Mediterranean legume that can be a good candidate as pioneer plants to limit regression of vegetation cover and loss of biodiversity in Tunisian arid soils. In order to grow legumes in such soils, pairing rhizobia and nodule associated bacteria (NAB) might provide numerous advantages. In this work, cultivable biodiversity of rhizobial symbionts and NAB in nodules of C. villosa plants growing in five arid regions of south Tunisia was characterized. Phylogenetic analysis using 16S rDNA gene, dnak, recA and nodD sequences separated nodule-forming bacteria in six clades associated to genera Ensifer, Neorhizobium, Phyllobacterium and Rhizobium. Among NAB, the strain Variovorax sp. CT7.15 was selected due to its capacity to solubilise phosphate and, more interestingly, its high level of aminocyclopropane-1-carboxylate deaminase (ACC deaminase) activity. C. villosa plants were inoculated with representative rhizobia of each phylogenetic group and co-inoculated with the same rhizobia and strain CT7.15. Compared with single rhizobia inoculation, co-inoculation significantly improved plant growth and nodulation, ameliorated plant physiological state and increased nitrogen content in the plants, independently of the rhizobia used. These results support the benefits of pairing rhizobia and selected NAB to promote legume growth in arid or degraded soils.

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Section: Rhizobia Isolated From C Villosa Nodules In Tunisian Arid Scontrasting

confidence: 79%

The ACC-Deaminase Producing Bacterium Variovorax sp. CT7.15 as a Tool for Improving Calicotome villosa Nodulation and Growth in Arid Regions of Tunisia

et al. 2020

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“…Although European, African and American species of Lupinus are generally considered to be nodulated by slow‐growing rhizobia affiliated to different Bradyrhizobium lineages (see Andrews and Andrews ; Stępkowski et al . for reviews), other reports have shown that the fast‐growing rhizobial species Phyllobacterium (Valverde et al . ), Ochrobactrum (Trujillo et al .…”

Section: Discussionmentioning

confidence: 99%

“…Lupinus species have been shown to be nodulated by slow-growing rhizobia of the genus Bradyrhizobium, including B. japonicum, B. lupini, B. canariense, B. diazoefficiens and B. elkanii species (Peix et al 2015;Shamseldin et al 2017;Vel azquez et al 2017;Ste z pkowski et al 2018;Mellal et al 2019). Also, fast-growing strains of the species Ochrobactrum lupini isolated from Lotus honoratus (Trujillo et al 2005), of the genus Microvirga from L. texensis (Ardley et al 2012), L. micranthus (Msaddak et al 2017a(Msaddak et al , 2017b and L. subcarnosus (Beligala et al 2017) and Burkholderia have been isolated from the later plant species.…”

Section: Introductionmentioning

confidence: 99%

Microvirgasp. symbiovar mediterranense nodulatesLupinus cosentiniigrown wild in Morocco

et al. 2019

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Aim To analyse the diversity of nodule‐forming bacteria isolated from Lupinus cosentinii naturally grown in the Maamora cork oak forest (Rabat, Morocco). Methods and Results Of the 31 bacterial strains, four were selected based on their REP‐PCR fingerprinting that were studied by sequencing and phylogenetic analysis of their 16S rRNA, gyrB, dnaK, recA and rpoB housekeeping genes as well as the nodC symbiotic gene. The nearly complete 16S rRNA gene sequence of the four representative strains showed that they are related to Tunisian strains of genus Microvirga isolated from L. micranthus with nucleotide identity values ranging from 98·67 to 97·13%. The single and concatenated sequences of the 16S rRNA, gyrB, dnaK, recA and rpoB housekeeping genes indicated that the L. cosentinii‐isolated strains had 99·2–99·9% similarities with the Tunisian L. micranthus microsymbionts. The nodC gene phylogeny revealed that the Moroccan strains clustered in the newly described mediterranense symbiovar, and nodulation tests showed that they nodulated not only L. cosentinii but also L. angustifolius, L. luteus and L. albus. Conclusions To the best of our knowledge, this is the first report concerning the isolation, molecular identification and phylogenetic diversity of L. cosentinii nodule‐forming endosymbionts and of their description as members of the Microvirga genus. Significance and Impact of the Study In this work, we show that Microvirga sp. can be isolated from root nodules of wild‐grown L. cosentinii in Northeast Africa, that selected strains also nodulate L. angustifolius, L. luteus and L. albus, and that they belong to symbiovar mediterranense. In addition, our data support that the ability of Microvirga to nodulate lupines could be related to the soil pH, its geographical distribution being more widespread than expected.

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“…The genetic diversity and community structure of rhizobia, which are natural microsymbionts of legumes, are commonly studied using a given leguminous plant as a trap plant, following the isolation of bacteria from nodules and their further characterization (genetic, functional, and phenotypic characterization). Such studies suggest that selective enrichment of rhizobial strains from the pool of the rhizobial community in the soil occurs based on symbiotic compatibility (gene background) with the plant, and depending on the plant used, the biodiversity of isolates can be restricted [78,79].…”

Section: In Vitro Assays In Vivo Assays: Tomatomentioning

confidence: 99%

Diversity and plant growth-promoting functions of diazotrophic/N-scavenging bacteria isolated from the soils and rhizospheres of two species of Solanum

et al. 2020

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Studies of the interactions between plants and their microbiome have been conducted worldwide in the search for growth-promoting representative strains for use as biological inputs for agriculture, aiming to achieve more sustainable agriculture practices. With a focus on the isolation of plant growth-promoting (PGP) bacteria with ability to alleviate N stress, representative strains that were found at population densities greater than 10 4 cells g -1 and that could grow in N-free semisolid media were isolated from soils under different management conditions and from the roots of tomato (Solanum lycopersicum) and lulo (Solanum quitoense) plants that were grown in those soils. A total of 101 bacterial strains were obtained, after which they were phylogenetically categorized and characterized for their basic PGP mechanisms. All strains belonged to the Proteobacteria phylum in the classes Alphaproteobacteria (61% of isolates), Betaproteobacteria (19% of isolates) and Gammaproteobacteria (20% of isolates), with distribution encompassing nine genera, with the predominant genus being Rhizobium (58.4% of isolates). Strains isolated from conventional horticulture (CH) soil composed three bacterial genera, suggesting a lower diversity for the diazotrophs/N scavenger bacterial community than that observed for soils under organic management (ORG) or secondary forest coverture (SF). Conversely, diazotrophs/N scavenger strains from tomato plants grown in CH soil comprised a higher number of bacterial genera than did strains isolated from tomato plants grown in ORG or SF soils. Furthermore, strains isolated from tomato were phylogenetically more diverse than those from lulo. BOX-PCR fingerprinting of all strains revealed a high genetic diversity for several clonal representatives (four Rhizobium species and one Pseudomonas species). Considering the potential PGP mechanisms, 49 strains (48.5% of the total) produced IAA (2.96-193.97 μg IAA mg protein -1 ), 72 strains (71.3%) solubilized FePO 4 (0.40-56.00 mg l -1 ), 44 strains (43.5%) solubilized AlPO 4 (0.62-17.05 mg l -1 ), and 44 strains produced siderophores (1.06-3.23). Further, 91 isolates (90.1% of total) showed at least one PGP trait, and 68 isolates (67.3%) showed multiple PGP traits. Greenhouse trials using the bacterial collection to inoculate tomato or lulo plants revealed increases in plant biomass (roots, shoots or both plant PLOS ONE | https://doi.org/10.

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Phylogeny and Phylogeography of Rhizobial Symbionts Nodulating Legumes of the Tribe Genisteae

Cited by 68 publications

References 168 publications

The ACC-Deaminase Producing Bacterium Variovorax sp. CT7.15 as a Tool for Improving Calicotome villosa Nodulation and Growth in Arid Regions of Tunisia

The ACC-Deaminase Producing Bacterium Variovorax sp. CT7.15 as a Tool for Improving Calicotome villosa Nodulation and Growth in Arid Regions of Tunisia

Microvirgasp. symbiovar mediterranense nodulatesLupinus cosentiniigrown wild in Morocco

Diversity and plant growth-promoting functions of diazotrophic/N-scavenging bacteria isolated from the soils and rhizospheres of two species of Solanum

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