2004
DOI: 10.1016/j.ympev.2004.06.010
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Phylogeny and intraspecific variability of holoparasitic Orobanche (Orobanchaceae) inferred from plastid rbcL sequences

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Cited by 53 publications
(45 citation statements)
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“…the average amounts of isolated DNA were 250-300 ng and the above counted contaminations were present in negligible amounts. primers the sixteen iSSR primers preselected by hristova et al (16) from primer Set #9 (University of British columbia, nucleic Acid-Protein Service Unit, nAPS Unit, http:// www.michaelsmith.ubc.ca/services/NAPS/ Primer_Sets/) were tested in this study. Because the production of Primer Set #9 was discontinued by UBC -NAPS Unit, the primers were ordered from Metabion International AG, Martinsried, Germany and upon arrival were dissolved in DNase-free water to 100 mmol final concentration.…”
Section: Dna Preparationmentioning
confidence: 99%
See 1 more Smart Citation
“…the average amounts of isolated DNA were 250-300 ng and the above counted contaminations were present in negligible amounts. primers the sixteen iSSR primers preselected by hristova et al (16) from primer Set #9 (University of British columbia, nucleic Acid-Protein Service Unit, nAPS Unit, http:// www.michaelsmith.ubc.ca/services/NAPS/ Primer_Sets/) were tested in this study. Because the production of Primer Set #9 was discontinued by UBC -NAPS Unit, the primers were ordered from Metabion International AG, Martinsried, Germany and upon arrival were dissolved in DNase-free water to 100 mmol final concentration.…”
Section: Dna Preparationmentioning
confidence: 99%
“…were grouped in the aggregate O. minor (7,9,12). According to Musselman (17,18) this aggregate consists of only one species, which displays wide morphological variability caused by the host plant and is poorly resolved even by broad-scale molecular phylogenetic analyses (16,23,26).…”
Section: Introductionmentioning
confidence: 99%
“…The Consortium for the Barcode of Life (CBOL) plant working group recommended the 2-locus combination of ribulose-1,5-bisphosphate carboxylase oxygenase large subunit (rbcL) and maturase K (matK) as the standard plant barcode based on assessments of recoverability, sequence quality and levels of species discrimination (CBOL Plant Working Group, 2009). Recently, several investigators used rbcL and matK sequences for barcoding or species identification (Asahina et al, 2010;Starr et al, 2009) as well as for phylogenetic analysis (Kuo et al, 2011;Manen et al, 2004;Tamura et al, 2004). However, universal barcode markers may not work in all cases (Roy et al, 2010).…”
Section: Introductionmentioning
confidence: 99%
“…A. melampodina revealed 13 specific bands, while A. pseudocotula and A. bornmuelleri showed 10 and 6 specific bands (SM), respectively. These bands may be possible specific markers after verifying that, individuals of each species appear the same description (Roman et al, 2003;Manen et al, 2004). Results of monomorphism and polymorphism of Anthemis using RAPD-analysis might be precise findings for the genetic diversity of genus Anthemis, to specify each species with the appearance of specific markers and definitive bands.…”
Section: Resultsmentioning
confidence: 90%