Phylogeny and divergence time estimation of cheilostome bryozoans based on mitochodrial 16S rRNA sequences

Previous analyses of the mitochondrial gene cytochrome c oxidase subunit 1 (COI) and γ‐proteobacterial endosymbiont diversity have suggested that the marine bryozoan Bugula neritina is a complex of three cryptic species, namely Types S, D and N. Types D and N were previously reported to have restricted distributions along California (western USA) and Delaware and Connecticut (eastern USA), respectively, whereas Type S is considered widespread in tropical, subtropical and temperate regions due to anthropogenic transport. Here, Bayesian species delimitation analysis of a data set composed of two mitochondrial (COI and large ribosomal RNA subunit [16S]) and two nuclear genes (dynein light chain roadblock type‐2 protein [DYN] and voltage‐dependent anion‐selective channel protein [VDAC]) demonstrated that Types S, D and N correspond to three biological species. This finding was significantly supported, in spite of the combinations of priors applied for ancestral population size and root age. Furthermore, COI sequences were used to assess the introduction patterns of the cosmopolitan Type S species. Two COI haplotypes of Type S (S1a and S1d) were found occurring at a global scale. Mantel tests showed correlation between these haplotypes and local sea surface temperature tolerance. Accordingly, the distributions of Type S haplotypes may reflect intraspecific temperature tolerance variation, in addition to the role of introduction vectors. Finally, we show that the Type N may also have been introduced widely, as this species was found for the first time in Central California and north‐eastern Australia.

show abstract

“…), with the exception of a few 16S sequences (Lim ; Hao et al . ; Okuyama et al . ; Lim‐Fong et al .…”

Section: Resultsmentioning

confidence: 99%

Cryptic species in the cosmopolitan Bugula neritina complex (Bryozoa, Cheilostomata)

et al. 2013

View full text Add to dashboard Cite

show abstract

“…The whole genomic DNA was extracted from thoracic muscle tissue with the DNeasy Blood & Tissue kit (Qiagen) after Hao et al (2005) [20]. Some universal PCR primers for short fragment amplifications of srRNA, COI, CytB, ND1, and COII genes were synthesized after Simon et al (1994) [21], Caterino and Sperling (1999) [22], and Simmons and Weller (2001) [23].…”

Section: Dna Extraction Pcr Amplification and Sequencingmentioning

confidence: 99%

Complete Mitogenomes of Euploea mulciber (Nymphalidae: Danainae) and Libythea celtis (Nymphalidae: Libytheinae) and Their Phylogenetic Implications

et al. 2013

Self Cite

View full text Add to dashboard Cite

The complete mitochondrial genome sequences of the two butterfly speciesEuploea mulciber(Lepidoptera: Nymphalidae: Danainae) andLibythea celtis(Lepidoptera: Nymphalidae: Libytheinae) were determined in this study, comprising 15,166 bp and 15,164 bp, respectively. The orientation and the gene order of the two mitogenomes are identical to those of most of the other lepidopteran species. All protein-coding genes ofEuploea mulciberandLibythea celtismitogenomes start with a typical ATN codon with the exception of COI gene which uses CGA as its initial codon. All tRNA genes possess the typical cloverleaf secondary structure except for tRNASer(AGN), which has a simple loop with the absence of the DHU stem. There are short microsatellite-like repeat regions, but no conspicuous macrorepeats scattered throughout the A + T-rich regions. Phylogenetic analysis among the available butterfly species suggests thatLibythea celtis(Libytheinae) is closely related toCalinaga davidis(Calinaginae), indicating that the subfamily Libytheinae may not represent a basal lineage of the Nymphalidae as previously suggested, and thatEuploea mulciberstands at the base of the nymphalid tree as a sister to all other nymphalids.

show abstract

“…Nilaparvata lugens is a major pest of rice, and it causes huge economic losses worldwide both through direct feeding and as a powerful vector of plant viruses (Zhao et al, 2005). Currently, the primary management strategy against N. lugens is frequently used insecticides include pymetrozine, imidacloprid, thiamethoxam, and buprofezin (Smith et al, 2008; He et al, 2011; Butler et al, 2012; Liu et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

NlCYP4G76 and NlCYP4G115 Modulate Susceptibility to Desiccation and Insecticide Penetration Through Affecting Cuticular Hydrocarbon Biosynthesis in Nilaparvata lugens (Hemiptera: Delphacidae)

Wang

Zhang

2019

Front. Physiol.

View full text Add to dashboard Cite

The functions of cuticular hydrocarbons (CHCs) are varied in insects, but one example is to reduce water loss. Previous work has suggested that biosynthesis of CHCs is strongly related to the CYP4G sub-family. Targeting these genes in the brown planthopper, Nilaparvata lugens Stål, might be a new application for integrated pest management. Therefore, we explored the functions of CYP4G76 (GenBank: KM217045.1) and CYP4G115 (GenBank: KM217046.1) genes in this study. The desiccation treatment (RH < 5%) for the duration of 1–3 days significantly increased the transcription level of CYP4G76 and CYP4G115 . RNAi through the injection of CYP4G76 and CYP4G115 dsRNA could significantly decrease their expression, respectively, and further reduced the biosynthesis of CHCs, i.e., saturated and straight-chain alkanes. When CYP4G76 and CYP4G115 were suppressed, the susceptibility of N. lugens nymphs to desiccation increased, due to the deficiency of the CHCs in the insect’s cuticle. When the expression of CYP4G76 and CYP4G115 was decreased, this resulted in an increased rate of penetration of the four insecticides: pymetrozine, imidacloprid, thiamethoxam and buprofezin. Therefore, CYP4G76 and CYP4G115 appear to regulate the biosynthesis of CHCs in N. lugens nymphs, which play a major role in protecting insects from water loss and the penetration of insecticides. CYP4G76 and CYP4G115 might be used as a novel target in integrated pest management to N. lugens.

show abstract

Phylogeny and divergence time estimation of cheilostome bryozoans based on mitochodrial 16S rRNA sequences

Cited by 12 publications

References 21 publications

Cryptic species in the cosmopolitan Bugula neritina complex (Bryozoa, Cheilostomata)

Cryptic species in the cosmopolitan Bugula neritina complex (Bryozoa, Cheilostomata)

Complete Mitogenomes of Euploea mulciber (Nymphalidae: Danainae) and Libythea celtis (Nymphalidae: Libytheinae) and Their Phylogenetic Implications

NlCYP4G76 and NlCYP4G115 Modulate Susceptibility to Desiccation and Insecticide Penetration Through Affecting Cuticular Hydrocarbon Biosynthesis in Nilaparvata lugens (Hemiptera: Delphacidae)

Contact Info

Product

Resources

About