“…We selected five chloroplast DNA regions from the large single copy (LSC), the small single copy (SSC) and the inverted repeat B (IR-B) regions of the genome: ndhF (coding region, SSC), rpl16 (coding region, intron, LSC), trnH-psbA (coding region, spacer, IR-B/LSC junction), encompassing the rps19 gene (Hiratsuka et al, 1989;Chang et al, 2006), trnL-trnF (coding region, intron, spacer, LSC) and trnS-trnG (coding region, spacer, LSC), encompassing the psbZ gene (e.g., see Fig. S1 in Besnard et al, 2013); and one nuclear DNA region: ITS (18S, 5.8S and 26S ribosomal RNA genes, and internal transcribed spacers 1 and 2). Primers used for amplification and sequencing, and PCR conditions are shown in Table 2. PCR products were cleaned using PEG 20% (polyethylene glycol; Paithankar and Prasad, 1991) and then sequenced in both directions using the Big Dye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Austin, Texas, USA) according to the following protocol: a hot start with 3 min of initial denaturation at 96°C, 30 cycles of 96°C denaturation for 20 s, 50°C annealing for 15 s, and 60°C extension for 4 min.…”