Phylogenomic analysis of the monkeypox virus (MPXV) 2022 outbreak: Emergence of a novel viral lineage?

Luna, Nicolás; Ramírez, Angie; Muñoz, Marina; Ballesteros, Nathalia; Patiño, Luz Helena; Castañeda, Sergio; Bonilla-Aldana, D. Katterine; Paniz‐Mondolfi, Alberto; Ramírez, Juan David

doi:10.1016/j.tmaid.2022.102402

Cited by 149 publications

(166 citation statements)

References 33 publications

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Section: Main Textmentioning

confidence: 79%

“…We could not exclude the possibility of genetic material loss resulting from freezing/thawing of extracted nucleic acids in the case of retrospective analyses. However recent phylogenetic analysis showed that MPXV genomes from the 2022 outbreak came from a new lineage called B.1 24 , confirming this hypothesis. This lack of MPXV genome detection before the identification of the first human cases would also strongly suggest the human origin of the viral genomes detected in the Parisian sewersheds and the absence of a pre-existing animal reservoir for MPXV.…”

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confidence: 79%

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First detection of Monkeypox virus genome in sewersheds in France

Wurtzer

Levert²,

Dhenain

et al. 2022

Preprint

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A monkeypox virus outbreak is currently spreading in multiple non-endemic countries since May 2022. The atypical clinical profile of patients has led to a very likely underestimation of the number of cases at the beginning of epidemic. The detection and quantification of the Monkeypox virus genome in sewersheds in Paris (France) correlated temporally with the identification of the first case of infection and the spread of the disease within the population connected to the sewage system.

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Section: Main Textmentioning

confidence: 79%

mentioning

confidence: 79%

First detection of Monkeypox virus genome in sewersheds in France

Wurtzer

Levert²,

Dhenain

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The B.1 lineage has signi cant 77383 nucleotide mutation of allele A. 15 However, there is no information available which can differentiate between the transmission pattern of A.2 and B.1 lineage.…”

Section: Discussionmentioning

confidence: 99%

First two cases of Monkeypox virus infection in travellers returned from UAE to India, July 2022

Yadav

Reghukumar

Sahay

et al. 2022

Preprint

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We report the first two confirmed cases of Monkeypox in foreign returnees from United Arab Emirates who presented with fever, myalgia, and vesicular lesions on the genital area with cervical lymphadenopathy. The oropharyngeal & nasopharyngeal swab, EDTA blood, serum, urine, lesion samples from multiple sites (lesion fluid, lesion roof and lesion base) were collected from both the cases on ninth post onset day of illness. The clinical specimens of both the cases were tested with real time PCR for Orthopoxvirus, Monkeypox virus (MPXV), West African clade specific MPXV. The specimens oropharyngeal & nasopharyngeal swab, urine, lesion samples from multiple sites (lesion fluid, lesion roof and lesion base) were tested positive for MPXV. The complete genome sequences obtained from skin lesions of case 1 and 2 showed similarity of 99.91 and 99.96% respectively with MPXV_USA_2022_FL001 West African clade. Phylogenetic analysis revealed that the two cases were infected with Monkeypox virus strain A.2 which belong to hMPXV-1A lineage of clade 3.

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“…Since May 2022, an increasing number of cases of Monkeypox have been reported, affecting non-endemic regions across the globe. Phylogenomic analysis of available MPXV genomes, performed to determine viral evolution and diversity, showed genomes from the ongoing 2022 multi-country outbreak clustering in a newly emerging clade harbouring (Luna et al, 2022). Indeed, we are now facing a multi-country outbreak, with about 18000 cases reported from 41 countries in the European region as of 9 August 2022 (ECDC-WHO, 2022).…”

Section: Discussionmentioning

confidence: 99%

Detection of Monkeypox virus DNA in the wastewater of an airport in Rome, Italy: expanding environmental surveillance to emerging threats

Rosa

Mancini

Veneri

et al. 2022

Preprint

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Since May 2022, monkeypox cases have been reported in non-endemic countries, and the disease was declared a Public Health Emergency of International Concern. Rapid identification of new cases is critical for outbreak control. Environmental surveillance can be used as a complementary tool for detecting pathogens circulating in communities. This study aimed to investigate whether Monkeypox DNA can be detected in wastewater from a transportation hub. Twenty samples were collected in Italy's first airport in Rome and tested using published and modified real-time PCR assays targeting the G2R region (TFN gene), F3L, and N3R genes. Nested PCR assays were also used for confirmation by sequencing. Three samples tested positive by real-time PCR and/or by nested PCR, confirming the occurrence of the virus in the airport's wastewater. Wastewater surveillance can be quickly adapted to investigate emerging threats and can be used to track the introduction and/or the diffusion of the Monkeypox virus in communities.

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Phylogenomic analysis of the monkeypox virus (MPXV) 2022 outbreak: Emergence of a novel viral lineage?

Cited by 149 publications

References 33 publications

First detection of Monkeypox virus genome in sewersheds in France

First detection of Monkeypox virus genome in sewersheds in France

First two cases of Monkeypox virus infection in travellers returned from UAE to India, July 2022

Detection of Monkeypox virus DNA in the wastewater of an airport in Rome, Italy: expanding environmental surveillance to emerging threats

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