Phylogenies of symbiotic genes of Bradyrhizobium symbionts of legumes of economic and environmental importance in Brazil support the definition of the new symbiovars pachyrhizi and sojae

Delamuta, Jakeline Renata Marçon; Menna, Pâmela; Ribeiro, Renan Augusto; Hungría, Mariangela

doi:10.1016/j.syapm.2017.04.005

Cited by 42 publications

(38 citation statements)

References 39 publications

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“… Bradyrhizobium is considered as the probable ancestor lineage of all rhizobia and, in the last decade, several studies reported remarkable genetic diversity within the genus, as well as symbiotic associations with an increasing number of legumes [e.g. [1, 2, 6–13]. The environmental and economic applications of Bradyrhizobium and its high diversity reassure the importance of characterization and seeking for strains with biotechnological potential.…”

Section: Full-textmentioning

confidence: 99%

“…The PCR products were purified using PureLinkTMQuick PCR Purification Kit (Invitrogen), according to the manufacturer’s procedure. Genes sequencing was performed in an ABI3500xL analyzer (Applied Biosystems), as described before [10], and the sequences were deposited at the GenBank database. The remaining sequences were retrieved from GenBank and all the accession numbers are shown in the phylograms and in Table S1 (available in the online version of this article).…”

Section: Phylogenymentioning

confidence: 99%

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Bradyrhizobium archetypum sp. nov., Bradyrhizobium australiense sp. nov. and Bradyrhizobium murdochi sp. nov., isolated from nodules of legumes indigenous to Western Australia

Helene

Klepa

O’Hara

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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The genus Bradyrhizobium is considered as the probable ancestor lineage of all rhizobia, broadly spread in a variety of ecosystems and with remarkable diversity. A polyphasic study was performed to characterize and clarify the taxonomic position of eight bradyrhizobial strains isolated from indigenous legumes to Western Australia. As expected for the genus, the 16S rRNA gene sequences were highly conserved, but the results of multilocus sequence analysis with four housekeeping genes (dnaK, glnII, gyrB and recA) confirmed three new distinct clades including the following strains: (1) WSM 1744T, WSM 1736 and WSM 1737; (2) WSM 1791T and WSM 1742; and (3) WSM 1741T, WSM 1735 and WSM 1790. The highest ANI values of the three groups in relation to the closest type strains were 92.4, 92.3 and 93.3 %, respectively, below the threshold of species circumscription. The digital DNA–DNA hybridization analysis also confirmed new species descriptions, with less than 52 % relatedness with the closest type strains. The phylogeny of the symbiotic gene nodC clustered the eight strains into the symbiovar retamae, together with seven Bradyrhizobium type strains, sharing from 94.2–98.1 % nucleotide identity (NI), and less than 88.7 % NI with other related strains and symbiovars. Morpho-physiological, phylogenetics, genomic and symbiotic traits were determined for the new groups and our data support the description of three new species, Bradyrhizobium archetypum sp. nov., Bradyrhizobium australiense sp. nov. and Bradyrhizobium murdochi sp. nov., with WSM 1744T (=CNPSo 4013T=LMG 31646T), WSM 1791T (=CNPSo 4014T=LMG 31647T) and WSM 1741T (=CNPSo 4020T=LMG 31651T) designated as type strains, respectively.

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Section: Full-textmentioning

confidence: 99%

Section: Phylogenymentioning

confidence: 99%

Bradyrhizobium archetypum sp. nov., Bradyrhizobium australiense sp. nov. and Bradyrhizobium murdochi sp. nov., isolated from nodules of legumes indigenous to Western Australia

Helene

Klepa

O’Hara

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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“…The genus Bradyrhizobium (Jordan, 1982) is considered the main rhizobial genus that establishes a symbiotic association with soybean (Hungria, Nogueira & Araujo, 2015;Sugiyama et al, 2015;Schmidt, Messmer & Wilbois, 2015). According to List of Prokaryotic Names with Standing in Nomenclature (LPSN, 2019), 41 species of Bradyrhizobium have already been described, with the species B. elkanii, B. japonicum, and B. diazoefficiens being the most used in commercial inoculants (Siqueira et al, 2014;Schmidt, Messmer & Wilbois, 2015;Delamuta et al, 2017). The Bradyrhizobium-soybean symbiosis is considered one of the most important natural relations exploited by the agricultural activity, since these bacteria can lead to grain yield increase and, consequently, eliminate or reduce the dependence on inorganic N fertilizers in crop cultivation (Chang, Lee & Hungria, 2015;Hungria, Marco & Ricardo, 2015;Collino et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Effects of plant growth-promoting rhizobacteria on co-inoculation with Bradyrhizobium in soybean crop: a meta-analysis of studies from 1987 to 2018

Zeffa

Fantin

Koltun

et al. 2020

PeerJ

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Background The co-inoculation of soybean with Bradyrhizobium and other plant growth-promoting rhizobacteria (PGPR) is considered a promising technology. However, there has been little quantitative analysis of the effects of this technique on yield variables. In this context, the present study aiming to provide a quantification of the effects of the co-inoculation of Bradyrhizobium and PGPR on the soybean crop using a meta-analysis approach. Methods A total of 42 published articles were examined, all of which considered the effects of co-inoculation of PGPR and Bradyrhizobium on the number of nodules, nodule biomass, root biomass, shoot biomass, shoot nitrogen content, and grain yield of soybean. We also determined whether the genus of the PGPR used as co-inoculant, as well as the experimental conditions, altered the effect size of the PGPR. Results The co-inoculation technology resulted in a significant increase in nodule number (11.40%), nodule biomass (6.47%), root biomass (12.84%), and shoot biomass (6.53%). Despite these positive results, no significant increase was observed in shoot nitrogen content and grain yield. The response of the co-inoculation varied according to the PGPR genus used as co-inoculant, as well as with the experimental conditions. In general, the genera Azospirillum, Bacillus, and Pseudomonas were more effective than Serratia. Overall, the observed increments were more pronounced under pot than that of field conditions. Collectively, this study summarize that co-inoculation improves plant development and increases nodulation, which may be important in overcoming nutritional limitations and potential stresses during the plant growth cycle, even though significant increases in grain yield have not been evidenced by this data meta-analysis.

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“…The amplified products were purified with PureLink ® Quick PCR Purification Kit (Invitrogen ® , Life Technologies ® , Germany). Sequencing was performed on a 3500XL Genetic Analyzer (Hitachi ® , Applied Biosystems ® , USA), as described before 30 and the gene sequences obtained were deposited in the GenBank.…”

Section: Methodsmentioning

confidence: 99%

Lettuce Production under Reduced Levels of N-fertilizer in the Presence of Plant Growth-promoting Bacillus spp. Bacteria

Venâncio¹,

Gomes²,

Nakatani³

et al. 2019

J Pure Appl Microbiol

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Lettuce consumption has increased worldwide due to the recognition of its nutritional value and to the consumers' search for healthier foods. Increased consumption must be coupled with more efficient production, demanding more nutrients. However, the utilization of chemical inputs in horticulture is decreasing, and biological approaches, such as the inoculation with plant growth-promoting bacteria (PGPB), are becoming more popular. Species of Bacillus have long been known promote the growth of plants, including lettuce, in agricultural soils, but little is known about their potential under Brazilian conditions. We have tested the ability of B. subtilis, B. pumilus, and B. amyloliquefaciens, carried in single or combined pre-commercial inoculants, to act as PGPB for field-grown lettuce plants of cultivar Elisa. In our experiments, the PGPB were challenged to promote plant growth when the recommended dose of nitrogen (N) fertilizer was reduced by 50%. Our results demonstrated that all species, either alone or combined, were able to improve plant vigor, the number of leaves, and the average diameter and weight of the lettuce heads, under the test conditions. Average gains in plant vigor and head weight due to all species were of the order of 50% relative to the control (no N fertilizer) treatment and, in most cases, gains due to the presence of the bacteria were superior to those promoted by the full (100%) N fertilization. This is the first report of positive utilization of pre-commercial products containing Bacillus for lettuce growth in Brazil.

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Phylogenies of symbiotic genes of Bradyrhizobium symbionts of legumes of economic and environmental importance in Brazil support the definition of the new symbiovars pachyrhizi and sojae

Cited by 42 publications

References 39 publications

Bradyrhizobium archetypum sp. nov., Bradyrhizobium australiense sp. nov. and Bradyrhizobium murdochi sp. nov., isolated from nodules of legumes indigenous to Western Australia

Bradyrhizobium archetypum sp. nov., Bradyrhizobium australiense sp. nov. and Bradyrhizobium murdochi sp. nov., isolated from nodules of legumes indigenous to Western Australia

Effects of plant growth-promoting rhizobacteria on co-inoculation with Bradyrhizobium in soybean crop: a meta-analysis of studies from 1987 to 2018

Lettuce Production under Reduced Levels of N-fertilizer in the Presence of Plant Growth-promoting Bacillus spp. Bacteria

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