Phylogenic position and marker studies using cpDNA of C. wightii: A critically endangered and medicinally important plant in India

Monpara, Jagdishchandra; Thaker, Vrinda S.

doi:10.1007/s42535-021-00199-w

Cited by 3 publications

(1 citation statement)

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“…The main aim of this work was to generate draft reference genomes through de novo genome assembly and the annotation of B. bipinnata, B. cuneata, and B. palmeri. We also mapped the obtained filtered reads against the chloroplast genomes of five Burseraceae species: Boswellia sacra [ 24 ], Canarium album [ 25 ], Commiphora wightii [ 26 ], C. gileadensis , and C. foliacea [ 24 ]. Additionally, for highlighting the utility of these genomes for subsequent population genetic and genomic studies, we identified nuclear genetic markers, such as simple sequence repeats (SSRs), in each of the assemblies, and single-nucleotide polymorphisms (SNPs) in a group of 43 Bursera individuals of the three species, using the assemblies as a reference.…”

Section: Introductionmentioning

confidence: 99%

Development of Genomic Resources in Mexican Bursera (Section: Bullockia: Burseraceae): Genome Assembly, Annotation, and Marker Discovery for Three Copal Species

Rico

Lorenzana²,

Benítez-Pineda

et al. 2022

Genes

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Bursera comprises ~100 tropical shrub and tree species, with the center of the species diversification in Mexico. The genomic resources developed for the genus are scarce, and this has limited the study of the gene flow, local adaptation, and hybridization dynamics. In this study, based on ~155 million Illumina paired-end reads per species, we performed a de novo genome assembly and annotation of three Bursera species of the Bullockia section: Bursera bipinnata, Bursera cuneata, and Bursera palmeri. The total lengths of the genome assemblies were 253, 237, and 229 Mb for B. cuneata, B. palmeri, and B. bipinnata, respectively. The assembly of B. palmeri retrieved the most complete and single-copy BUSCOs (87.3%) relative to B. cuneata (86.5%) and B. bipinnata (76.6%). The ab initio gene prediction recognized between 21,000 and 32,000 protein-coding genes. Other genomic features, such as simple sequence repeats (SSRs), were also detected. Using the de novo genome assemblies as a reference, we identified single-nucleotide polymorphisms (SNPs) for a set of 43 Bursera individuals. Moreover, we mapped the filtered reads of each Bursera species against the chloroplast genomes of five Burseraceae species, obtaining consensus sequences ranging from 156 to 160 kb in length. Our work contributes to the generation of genomic resources for an important but understudied genus of tropical-dry-forest species.

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