Phylogenic analysis of the genus Leishmania by cytochrome b gene sequencing

Asato, Yutaka; Oshiro, Minoru; Myint, Chomar Kaung; Yamamoto, Yu; Kato, Hirotomo; Marco, Jorge Diego; Mimori, Tatsuyuki; Gómez, Eduardo A.; Hashiguchi, Yoshihisa; Uezato, Hiroshi

doi:10.1016/j.exppara.2008.12.013

Cited by 79 publications

(87 citation statements)

References 20 publications

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“…3), suggesting that the Bayesian inference could be properly applied to the phylogenetic analyses of subgenus Leishmania. Considering congruent with the recent studies on the interrelationships of subgenus Leishmania (Asato et al 2009;Fraga et al 2010), we tentatively support the relationships inferred from BI of the dataset incorporating indels coding (Fig. 5) as the preferred phylogeny.…”

Section: Discussionsupporting

confidence: 72%

“…To overcome these problems, DNA-based methods have been widely used for Leishmania spp. identification and/or phylogenetic reconstruction with a variety of targets such as proteincoding genes, non-coding segments, microsatellites, and restriction fragment length polymorphisms (e.g., Piarroux et al 1995;Noyes et al 1997;El Tai et al 2001;Ibrahim and Barker 2001;Kuhls et al 2005;Asato et al 2009;Montalvo et al 2010). Notably, Lukeš et al (2007) went a long way to resolving the issues for the Leishmania donovani complex.…”

Section: Introductionmentioning

confidence: 99%

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Species discrimination and phylogenetic inference of 17 Chinese Leishmania isolates based on internal transcribed spacer 1 (ITS1) sequences

Yang

Guo

et al. 2010

Parasitol Res

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Leishmaniasis is a geographically widespread disease, caused by protozoan flagellates of the genus Leishmania. This disease still remains endemic in China, especially in the west and northwest frontier regions. To date, the phylogenetic relationships among Chinese Leishmania isolates are still unclear, and the possible taxonomic diversity remains to be established. In this study, the ITS1-5.8S fragments of ten isolates collected from different foci in China were determined. To infer the phylogenetic relationships among them, seven sequences of Chinese Leishmania isolates retrieved from GenBank were also included. Both parsimony and Bayesian analyses reveal an unexpected but strongly supported clade comprising eight newly determined isolates, which is sister to other members of subgenus Leishmania. In combination with genetic distance analysis, this provides evidence of the occurrence of an undescribed species of Leishmania. Our results also suggest that (1) the isolate IPHL

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Section: Discussionsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Species discrimination and phylogenetic inference of 17 Chinese Leishmania isolates based on internal transcribed spacer 1 (ITS1) sequences

Yang

Guo

et al. 2010

Parasitol Res

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“…We think that this grouping is logical because it is in accord with the current taxonomic scheme of Leishmania (WHO 1990). In addition, the interrelationship of L. major, L. gerbilli, and L. turanica is also supported by the cyt b phylogeny (Asato et al 2009) and ITS1 genealogy (Yang et al 2010). Accordingly, we believe that the isolate MRHO/ CN/62/GS-GER20 is L. gerbilli.…”

Section: Discussionmentioning

confidence: 78%

“…However, their phylogenetic hypotheses were only deduced from about 400-bp ITS1-5.8S segments. On the other hand, as shown by simulations and empirical studies, more taxa and data do have effect on the phylogenetic reconstruction (e.g., Pollock et al 2002;Zwickl and Hillis 2002;Botilde et al 2006;Asato et al 2009;Fraga et al 2010). Such a perspective is therefore still far from clear, promoting us to pursue further studies on the classification and phylogeny of Chinese Leishmania isolates.…”

Section: Introductionmentioning

confidence: 99%

Species delimitation and phylogenetic relationships of Chinese Leishmania isolates reexamined using kinetoplast cytochrome oxidase II gene sequences

et al. 2011

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Leishmaniasis is a geographically widespread disease caused by protozoan parasites belonging to the genus Leishmania and transmitted by certain species of sand fly. This disease still remains endemic in China, especially in the west and northwest frontier regions. A recent ITS1 phylogeny of Chinese Leishmania isolates has challenged some aspects for their traditional taxonomy and cladistic hypotheses of their phylogeny. However, disagreement with respect to relationships within Chinese Leishmania isolates highlights the need for additional data and analyses. Here, we test the phylogenetic relationships among Chinese isolates and their relatives by analyzing kinetoplast cytochrome oxidase II (COII) gene sequences, including 14 Chinese isolates and three isolates from other countries plus 17 sequences retrieved from GenBank. The COII gene might have experienced little substitution saturation, and its evolutionary process was likely to have been stationary, reversible, and homogeneous. Both neighbor-joining and Bayesian analyses reveal a moderately supported group comprising ten newly determined isolates, which is closely related to Leishmania tarentolae and Endotrypanum monterogeii. In combination with genetic distance analysis as well as Bayesian hypothesis testing, this further corroborates the occurrence of an undescribed species of Leishmania. Our results also suggest that (1) isolate MHOM/CN/93/GS7 and isolate IPHL/CN/77/XJ771 are Leishmania donovani; (2) isolate MHOM/CN/84/JS1 is Leishmania tropica; (3) the status referring to an isolate MRHO/CN/62/GS-GER20 from a great gerbil in Gansu, China, as Leishmania gerbilli, formerly based on multilocus enzyme electrophoresis, is recognized; and (4) E. monterogeii is nested within the genus Leishmania, resulting in a paraphyletic Leishmania. In addition, the results of this study enrich our understanding of the heterogeneity and relationships of Chinese Leishmania isolates.

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“…For the classification of Leishmania species, several genetic markers have been studied. 15,17,[25][26][27][28][29][30][31] FTA cards were recently used for the less invasive dry collection of specimens from patients with CL. reported as a principal pathogen in this endemic area.…”

Section: Discussionmentioning

confidence: 99%

Leishmania species identification using FTA card sampling directly from patients’ cutaneous lesions in the state of Lara, Venezuela

Kato¹,

Watanabe²,

Nieto³

et al. 2011

Transactions of the Royal Society of Tropical Medicine and Hygi

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Phylogenic analysis of the genus Leishmania by cytochrome b gene sequencing

Cited by 79 publications

References 20 publications

Species discrimination and phylogenetic inference of 17 Chinese Leishmania isolates based on internal transcribed spacer 1 (ITS1) sequences

Species discrimination and phylogenetic inference of 17 Chinese Leishmania isolates based on internal transcribed spacer 1 (ITS1) sequences

Species delimitation and phylogenetic relationships of Chinese Leishmania isolates reexamined using kinetoplast cytochrome oxidase II gene sequences

Leishmania species identification using FTA card sampling directly from patients’ cutaneous lesions in the state of Lara, Venezuela

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