Phylogenic analysis of human bocavirus detected in children with acute respiratory infection in Yaounde, Cameroon

Kenmoe, Sébastien; Vernet, Marie‐Astrid; Njankouo‐Ripa, Mohamadou; Penlap, Véronique; Vabret, Astrid; Njouom, Richard

doi:10.1186/s13104-017-2620-y

Cited by 16 publications

(13 citation statements)

References 27 publications

(55 reference statements)

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“…Sequence analysis of the partial NP1, VP1/VP2 genes obtained from the HBoV-positive patients revealed that all 36 Kuwaiti strains belonged to the HBoV-1 genotype, with HBoV-2, HBoV-3 and HBoV-4 not being detected (). These results are in agreement with other studies, which have shown that HBoV-1 is a respiratory pathogen and is associated with respiratory illness, while HBoV-2, 3 and 4 are not directly involved in respiratory tract infections [15, 24, 40]. On the other hand, some reports have described an HBoV2–4 prevalence of 0.4–4.3 % in respiratory samples, but their roles in respiratory diseases are uncertain [41, 42].…”

Section: Resultssupporting

confidence: 92%

Human bocavirus (HBoV) in Kuwait: molecular epidemiology and clinical outcome of the virus among patients with respiratory diseases

Madi

Al-Adwani

2020

Journal of Medical Microbiology

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Introduction. Globally, human bocavirus (HBoV) has been detected in respiratory samples from patients suffering from upper and lower respiratory diseases. In Kuwait, little is known about the epidemiological and clinical characterization of the virus and genetic characterization of the virus as a respiratory pathogen is unknown. Aim. This study aims to explore the molecular epidemiology and clinical features of HBoV isolates in patients with respiratory diseases. Methodology. Retrospectively, between 2018 and 2020, 5941 respiratory samples from patients with respiratory diseases were screened for respiratory viruses using multiplex real-time PCR. Samples that were positive for HBoV were then subjected to NP1 and VP1/PV2 phylogenetic analysis. Results. HBoV was detected in 1.9 % of the patients, with a peak incidence of infection among children <1 year old. Co-infection with other respiratory viruses was observed in 56.8 % of HBoV-positive patients. Fever, cough and respiratory distress were the most common clinical features of HBoV infection. Phylogenetic analysis of the Kuwaiti HBoV isolates revealed that all the isolates were of the HBoV-1 genotype, with slight sequence variations among the isolates. Conclusion. This study illustrated the predominance of the HBoV-1 genotype in patients with respiratory diseases in Kuwait with minimal genetic variability. It also highlighted the clinical features of HBoV-1 infection, verifying its role in respiratory diseases.

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Section: Resultssupporting

confidence: 92%

Human bocavirus (HBoV) in Kuwait: molecular epidemiology and clinical outcome of the virus among patients with respiratory diseases

Madi

Al-Adwani

2020

Journal of Medical Microbiology

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“…The six isolates that clustered closely to the references strains for HBoV-1 (Figure 1 & 2) were gotten from children that exhibited symptoms of high severity (Table 4 & 5). This is similar to the findings of Kenmoe et al and Pogka et al, whose study indicated that HBoV-1 isolates that aligns with the HBoV-1 reference strains are often gotten from children showing symptoms of severe acute respiratory tract infections (23,24). This could be because HBoV -1 reference strains were isolated from children with severe acute respiratory tract illness by Allander et al (3).…”

Section: Discussionsupporting

confidence: 84%

Human Bocavirus Infection Among Children with Respiratory Tract Infection in Ibadan, Nigeria

Joseph¹,

Faneye²,

Adeniji³

2019

Preprint

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Background: Human Bocavirus (HBoV) which is a single stranded DNA virus of the family Parvoviridae, is responsible for 21.5% of childhood respiratory tract infections (RTI) annually. Among the four genotypes currently known, HBoV1 has been associated with acute respiratory tract infection. Although, there have been studies on HBoV in some countries, there is limited information on this virus in sub-Saharan Africa where there is the highest burden of RTI. This study aimed to characterize the circulating strains of HBoV in Ibadan, Nigeria. Methods: Nasopharyngeal and oropharyngeal swab samples were collected from 333 children ≤5 years old presenting with RTI attending hospitals in Ibadan, whose parents assented from 2014 – 2015. HBoV isolates were sequenced after a nested PCR and phylogenetic analysis was carried out using Mega 6 software. The HBoV isolates of this study has been assigned the Genbank accession numbers KY701984 – KY702006. Results: A total of 27 children tested positive for HBoV by PCR and 23 of the 27 isolates were successfully sequenced. The 23 HBoV isolates from this study were type as HBoV-1 genotype by phylogenetic analysis. Phylogram analysis indicated that the isolates belong to the same clades. Six Isolates aligned closely to the reference strains ST1 and ST2, while 17 isolates showed a high divergence to the reference isolates. Conclusion: This study shows the contribution on HBoV in respiratory tract infections in Nigeria and that HBoV1 strain is the strain associated with the infection.

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“…HBoV1 infected cases were associated with high rates of other respiratory viral coinfections. Several studies revealed that rates of detection of HBoV in acute respiratory infection (ARI) is about 3-19.0% [13,14]. In the present study, the overall detection rate of HBoV in among hospitalized children due to LRTI was 6.63% which was similar to other countries of the world.…”

Section: Discussionsupporting

confidence: 82%

Human Bocavirus in Saudi Arabia: Molecular Epidemiology and Co-Infections Among Children With Acute Respiratory Tract Infections

Hamed

Farrag

Amer

et al. 2020

Preprint

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Background During the last few years since HBoV discovery, the virus has attracted less attention and appeared to be one of the most common acute respiratory tract pathogens in children. However, few studies focused on genetic characterization and epidemiology of HBoV in Saudi Arabia, leading us to evaluate the molecular epidemiology of HBoV strains among children with ARI. This study was designed to study the epidemiological, genetic diversity and circulation pattern of HBoV among hospitalized children (< 7 years) due to acute respiratory tract infection (ARTI) during two consecutive years. Nasopharyngeal aspirate (NPA) samples (n=196) were collected. HBoV and co-infection with other respiratory pathogens were identified using qRT-PCR. Positive samples were subsequently subjected to amplify full-length of VP1/VP2 gene, followed by sequencing and phylogenetic analyses. Results Our data showed that 3.4% (2014/2015) and 11.3% (2015/2016) of children hospitalized due to acute respiratory infections (ARI) were infected by HBoV-1. We have shown that HBoV-1 was detected year-round. HBoV-1 also was co-detected with one or multiple other respiratory viruses. Phylogenetic analysis of complete HBoV VP1/2 sequences showed no nucleotide variation and the circulation of HBoV-1. The multisequence analysis showed complete identity between HBoV isolated from 2014/2015 and 2015/2016, suggesting no genetic variability among the local circulating HBoV-1 genotypes. Our data reveal that genetically conserved HBoV-1 is circulating in Saudi Arabia. Conclusions The multisequence analysis showed complete identity between HBoV-1 isolated from 2014/2015 and 2015/2016, suggesting no genetic variability among the local circulating HBoV-1 genotypes. Our data reveal that genetically conserved HBoV-1 is circulating year-round in Saudi Arabia.

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Phylogenic analysis of human bocavirus detected in children with acute respiratory infection in Yaounde, Cameroon

Cited by 16 publications

References 27 publications

Human bocavirus (HBoV) in Kuwait: molecular epidemiology and clinical outcome of the virus among patients with respiratory diseases

Human bocavirus (HBoV) in Kuwait: molecular epidemiology and clinical outcome of the virus among patients with respiratory diseases

Human Bocavirus Infection Among Children with Respiratory Tract Infection in Ibadan, Nigeria

Human Bocavirus in Saudi Arabia: Molecular Epidemiology and Co-Infections Among Children With Acute Respiratory Tract Infections

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