Phylogenetic study on Proales daphnicola Thompson, 1892 (Proalidae) and its relocation to Epiphanes (Rotifera: Epiphanidae)

Wilts, Eike F.; Bruns, Darla; Fontaneto, Diego; Ahlrichs, Wilko H.

doi:10.1016/j.jcz.2011.08.005

Cited by 6 publications

(4 citation statements)

References 32 publications

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“…Molecular phylogenies have been recently used also to help taxonomists solving controversies at lower systematic hierarchies. Examples of the successful use of molecular phylogenies in taxonomy can be found in the clarification of the position of the genus Plationus in the family Brachionidae , in the reallocation of Proales daphnicola to the genus Epiphanes , and in the support for the description of new species in the genus Abrochtha . Given the increased availability of genetic information, the low cost of sequencing and the fact that most laboratories nowadays have access to sequencing facilities, it is safe to foresee that the use of molecular phylogenies will be more and more often incorporated in rotifer taxonomy and systematics, especially in solving species complexes in taxa with known extensive morphological variability of unclear taxonomic value.…”

Section: The Phylogenetic Relationships Within Rotiferamentioning

confidence: 99%

Molecular phylogenies as a tool to understand diversity in rotifers

Fontaneto

2014

Internat. Rev. Hydrobiol.

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Over the past decade, the availability of DNA sequence data from rotifers and the development of statistical and analytical tools linked to phylogenetic reconstructions allowed researchers to deepen our knowledge of patterns and processes in rotifer diversity. Here, I review the recent rotifer literature regarding: (i) the position of rotifers in the animal kingdom, with the wide consensus being within Spiralia, in a clade called Platyzoa together with Acanthocephalans, Gastrotricha, Gnathostomulida, Micrognathozoa, and Platyhelminthes; (ii) the still unresolved phylogenetic relationships within rotifers and the disputed position of Acanthocephala; (iii) the discovery of extensive hidden diversity through DNA taxonomy; (iv) phylogeography; and (v) the phylogenetic structure of rotifer communities. In order to facilitate the further discovery of hidden diversity in rotifers, I provide a simple tutorial on the currently available methods in DNA taxonomy from single locus data using a previously published rotifer dataset as an example. Moreover, I outline a brief perspective on the potentiality of the use of phylogenetic approaches in future rotifer studies.

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Section: The Phylogenetic Relationships Within Rotiferamentioning

confidence: 99%

Molecular phylogenies as a tool to understand diversity in rotifers

Fontaneto

2014

Internat. Rev. Hydrobiol.

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“…For example, Wilts et al. ( 2012 ) using the primers COI-F/COI-R investigated the phylogeny of Proales daphnicola Thompson, 1892 (Proalidae) and relocated it into Epiphanes . To avoid the problems of COI amplification, Hirai ( 2013 ) proposed to use 28S rRNA instead.…”

Section: Introductionmentioning

confidence: 99%

A new set of highly efficient primers for COI amplification in rotifers

Zhang

Sun

et al. 2021

Mitochondrial DNA Part B

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Rotifers are a small-sized but key group of freshwater zooplankters with high species richness, linking primary producers to higher consumers in aquatic food webs. DNA barcoding has been widely used in exploring its biodiversity, cryptic speciation and phylogeny. However, the inefficiency of universal primers to amplify COI of rotifers hinders our understanding of their species richness and genetic diversity. Here, we develop a new pair of primers, 30 F and 885 R, to amplify the COI gene of rotifers. We used 22 species to test their PCR success rate and found that the new pair of primers was more efficient (86%) than two pairs of universal primers, namely, dgLCO and dgHCO (32%), and Folmer primers (59%). The new primers will allow the barcoding of groups that were so far difficult to sequence and will contribute to clarify species diversity and phylogeny of rotifers.

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“…Meyer et al [26] modified the Folmer primers to obtain the primers dgLCO/dgHCO and amplified the COI sequence of the B. plicatilis complex [24,27]. Wilts et al [28] developed primers COI-F/COI-R to amplify the COI of Proales daphnicola (Thompson, 1892) [25,27,29]. Elías-Gutiérrez et al [30] used the Zplank primers to amply rotifer COI and obtained 11 BINs (Barcode Index Numbers) of rotifers, with a sequencing success rate of 100%.…”

Section: Introductionmentioning

confidence: 99%

Application of COI Primers 30F/885R in Rotifers to Regional Species Diversity in (Sub)Tropical China

Zhang

Huang

et al. 2021

Diversity

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Rotifers are the most diverse group in freshwater zooplankton and play an important role in food webs and ecosystems. DNA barcoding has become a useful approach to investigate species diversity at local and regional scales, but its application is still limited by efficient primers for the group. To test a pair of primers 30F/885R recently designed for rotifers, we applied them to investigating regional species diversity in the freshwater of South China. We sequenced the cytochrome c oxidase subunit I (COI) gene of rotifers collected from the investigated 23 reservoirs in a large river basin and obtained 145 COI sequences from 33 species in 14 genera. The mean PCR success rate for all tested species was 50%. The 145 sequenced mtCOI in this study covered 33 of 64 identified morphological taxa, including most of the common species in the basin. The intraspecific genetic distance was calculated with a K2P model for 24 rotifer species occurring in the quantitative samples, in which 15 rotifers, such as Keratella cochlearis and Brachionus calyciflorus, had a genetic distance higher than 5%. The high intraspecific genetic differentiation indicates that cryptic species are probably common in (sub)tropical China.

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Phylogenetic study on Proales daphnicola Thompson, 1892 (Proalidae) and its relocation to Epiphanes (Rotifera: Epiphanidae)

Cited by 6 publications

References 32 publications

Molecular phylogenies as a tool to understand diversity in rotifers

Molecular phylogenies as a tool to understand diversity in rotifers

A new set of highly efficient primers for COI amplification in rotifers

Application of COI Primers 30F/885R in Rotifers to Regional Species Diversity in (Sub)Tropical China

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