Phylogenetic study on Shiraia bambusicola by rDNA sequence analyses

Abstract

In this study, 18S rDNA and ITS-5.8S rDNA regions of four Shiraia bambusicola isolates collected from different species of bamboos were amplified by PCR with universal primer pairs NS1/NS8 and ITS5/ITS4, respectively, and sequenced. Phylogenetic analyses were conducted on three selected datasets of rDNA sequences. Maximum parsimony, distance and maximum likelihood criteria were used to infer trees. Morphological characteristics were also observed. The positioning of Shiraia in the order Pleosporales was well supported by bootstrap, which agreed with the placement by Amano (1980) according to their morphology. We did not find significant inter-hostal differences among these four isolates from different species of bamboos. From the results of analyses and comparison of their rDNA sequences, we conclude that Shiraia should be classified into Pleosporales as Amano (1980) proposed and suggest that it might be positioned in the family Phaeosphaeriaceae.

Pleosporales

“…Based on the SSU and ITS rDNA sequences analysis, its pleosporalean status was verified, and Shiraia was suggested to be closely related to Leptosphaeriaceae and/or Phaeosphaeriaceae ( Pleosporineae ) (Cheng et al 2004). Based on the molecular phylogenetic analysis, another Shiraia-like fungus was reported which produced distinctive prawn-shaped conidioma-like structures (Morakotkarn et al 2008), and differed from conidiomata in the anamorph of S. bambusicola described by Liu (2009).…”

Molecular diversity of bamboo-associated fungi isolated from Japan

“…In Pleosporales, S. bambusicola was recorded as a parasitic fungus on the twigs of bamboo plants; its uses in Chinese medicine have been documented (Kishi et al, 1991;Cheng et al, 2004). This species is widely distributed in Southern China and has been reported in Japan, along with Phaeosphaeria and Leptosphaeria.…”

Taxonomic characterization of Shiraia-like fungi isolated from bamboos in Japan

“…The stroma of this species has been used as a traditional medicine in China (Cheng et al 2004). To date, extensive studies have been conducted on compounds of S. bambusicola, which have been found to be involved in antitumor activity and antiangiogenesis (Mazzini et al 2001;Tong et al 2004;Chen et al 2005).…”

“…Taxonomic characterization of Shiraia-like fungi isolated from bamboos in Japan were analyzed by Cheng et al (2004). In addition, Tsuda and Ueyama (1987) described the presence of the synamorph with macro-and microconidia.…”