Phylogenetic study of metallo-β-lactamase producing multidrug resistant Pseudomonas aeruginosa isolates from burn patients

Jena, J.; Debata, Nagen Kumar; Sahoo, Rajesh Kumar; Subudhi, Enketeswara

doi:10.1016/j.burns.2015.06.004

Cited by 8 publications

(5 citation statements)

References 24 publications

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“…Pseudomonasaeruginosa has been frequently isolated from the burnt patients in the recent studies [22,38,39]. We also observed thatPseudomonas spp., was the most abundantly isolated bacterial pathogen from 49.33 % burnt patients.…”

Section: Leseva Et Al Reported a Positive Correlation Between The Msupporting

confidence: 66%

Risk factors analysis: screening of extended-spectrum-β-lactamase producing Gram-negative isolates of burn infections from tertiary care hospital Lahore, Pakistan

Haider

Ain

Hussain

et al. 2019

Preprint

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Background Burnt patients are highly susceptible to invasion of multidrug resistant strains after the skin damage. The main objective of this study was to estimate the frequency of ESBLs producing Gram-negative in post-burn infections and its correlation with different risk factors (age, gender, socio-economic status, burns etiology etc.) Methods The descriptive case-series study was conducted at Jinnah Hospital Lahore’s Burn and Reconstructive Surgery Centre (JB & RSC) and the Department of Microbiology and Molecular Genetics (MMG), University of the Punjab, Lahore. The clinical specimens of wound swabs, biopsy, and blood were collected from 300 patients during 12th August, 2017 to 12th August 2018. The cephalosporins resistant Gram-negative isolates were further analyzed. The clinical history of burnt patients was recorded which included the age, gender, socio-economic status, residence, occupation, hospital stay, wards, burn degree, total burnt surface area (TBSA%), etiology of burn and other factors. CLSI guidelines 2017 were followed for the antimicrobial susceptibility testing (AST) and ESBLs screening. Results Pseudomonas spp. were the most-frequently isolated 49.33% (n=74) followed by 22.67% (n=34) Klebsiella spp., and 20.00% (n=30) Acinetobacter spp., strains. Pseudomonas spp., were the most frequently isolated from burnt specimens 70 (46.67%) having a significant correlation (x2=24.11, p < 0.001). Community acquired infections were observed in 50.70% (n=76) and nosocomial infections in 49.30% (n=74) patients. Burnt people having the age of ≤ 29 years were found to be significantly associated with the MDR infections (x2=24.96, p = 0.003). Low socio-economic status, longer hospitalization and all other risk factors had non-significant correlation (p > 0.05). A large fraction of the isolates 86.00% (n=129) were not confirmed as ESBLs producers by phenotypic screening. Conclusion It has been concluded that Gram-negative MDR strains are rapidly causing infections in burnt patients and need to be properly alleviated. The high frequency of multidrug resistant Pseudomonas spp., was associated with the burn infections. The patients belonging to young age were significantly found to be infected by MDR strain after burns.

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Section: Leseva Et Al Reported a Positive Correlation Between The Msupporting

confidence: 66%

Risk factors analysis: screening of extended-spectrum-β-lactamase producing Gram-negative isolates of burn infections from tertiary care hospital Lahore, Pakistan

Haider

Ain

Hussain

et al. 2019

Preprint

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“…The results were evaluated according to the values of the control (data not shown). Ciprofloxacin and fluconazole MIC values were within the accuracy range for the CLSI throughout the study [ 35 ]. Olive leaf extract obtained at SFMAE optimum conditions showed antibacterial activity against S. aureus and S. epidermidis , with a MIC value of 1250 µg/mL.…”

Section: Resultsmentioning

confidence: 99%

Solvent-Free Microwave-Assisted Extraction of Polyphenols from Olive Tree Leaves: Antioxidant and Antimicrobial Properties

et al. 2017

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Response surface methodology (RSM) and artificial neural networks (ANN) were evaluated and compared in order to decide which method was the most appropriate to predict and optimize total phenolic content (TPC) and oleuropein yields in olive tree leaf (Olea europaea) extracts, obtained after solvent-free microwave-assisted extraction (SFMAE). The SFMAE processing conditions were: microwave irradiation power 250–350 W, extraction time 2–3 min, and the amount of sample 5–10 g. Furthermore, the antioxidant and antimicrobial activities of the olive leaf extracts, obtained under optimal extraction conditions, were assessed by several in vitro assays. ANN had better prediction performance for TPC and oleuropein yields compared to RSM. The optimum extraction conditions to recover both TPC and oleuropein were: irradiation power 250 W, extraction time 2 min, and amount of sample 5 g, independent of the method used for prediction. Under these conditions, the maximal yield of oleuropein (0.060 ± 0.012 ppm) was obtained and the amount of TPC was 2.480 ± 0.060 ppm. Moreover, olive leaf extracts obtained under optimum SFMAE conditions showed antibacterial activity against S. aureus and S. epidermidis, with a minimum inhibitory concentration (MIC) value of 1.25 mg/mL.

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“… Egypt [ 26 ] A. baumanii n.r. France [ 27 ] A. baumanii OXA-58 India [ 28 ] K. pneumoniae OXA-48&NDM [ 29 , 30 ] P. aeruginosa n.r. [ 30 ] A. baumanii n.r.…”

Section: Resultsunclassified

Extensive colonization with carbapenemase-producing microorganisms in Romanian burn patients: infectious consequences from the Colectiv fire disaster

Pirii

Friedrich

Rossen

et al. 2017

Eur J Clin Microbiol Infect Dis

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Health care of severe burn patients is highly specialized and may require international patient transfer. Burn patients have an increased risk of developing infections. Patients that have been hospitalized in countries where carbapenemase-producing microorganisms (CPMO) are endemic may develop infections that are difficult to treat. In addition, there is a risk on outbreaks with CPMOs in burn centers. This study underlines that burn patients may extensively be colonized with CPMOs, and it provides best practice recommendations regarding clinical microbiology and infection control. We evaluated CPMO-carriage and wound colonization in a burn patient initially treated in Romania, and transported to the Netherlands. The sequence types and acquired beta-lactamase genes of highly-resistant microorganisms were derived from next generation sequencing data. Next, we searched literature for reports on CPMOs in burn patients. Five different carbapenemase-producing isolates were cultured: two unrelated OXA-48-producing Klebsiella pneumoniae isolates, OXA-23-producing Acinetobacter baumanii, OXA-48-producing Enterobacter cloacae, and NDM-1-producing Providencia stuartii. Also, multi-drug resistant Pseudomonas aeruginosa isolates were detected. Among the sampling sites, there was high variety in CPMOs. We found 46 reports on CPMOs in burn patients. We listed the epidemiology of CPMOs by country of initial treatment, and summarized recommendations for care of these patients based on these reports and our study.

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Phylogenetic study of metallo-β-lactamase producing multidrug resistant Pseudomonas aeruginosa isolates from burn patients

Cited by 8 publications

References 24 publications

Risk factors analysis: screening of extended-spectrum-β-lactamase producing Gram-negative isolates of burn infections from tertiary care hospital Lahore, Pakistan

Risk factors analysis: screening of extended-spectrum-β-lactamase producing Gram-negative isolates of burn infections from tertiary care hospital Lahore, Pakistan

Solvent-Free Microwave-Assisted Extraction of Polyphenols from Olive Tree Leaves: Antioxidant and Antimicrobial Properties

Extensive colonization with carbapenemase-producing microorganisms in Romanian burn patients: infectious consequences from the Colectiv fire disaster

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