Phylogenetic Study of Hypoxylon and Related Genera Based on Ribosomal ITS Sequences

Sánchez-Ballesteros, Javier; González, Vicente Ferreira; Salazar, Óscar; Acero, Francisco Javier; Portal, Maria A.; Julián, Marı́a C.; Rubio, Víctor J.; Bills, Gerald F.; Polishook, Jon D.; Platas, Gonzalo; Mochales, S.; Peláez, Fernando

doi:10.2307/3761591

Cited by 60 publications

(21 citation statements)

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“…The separation of hypoxyloid and xylarioid clades generated in this study is generally concordant with chemotaxonomy and their specific asexual morphs (Sánchez-Ballesteros et al 2000, Smith et al 2003, Triebel et al 2005. Clade A (hypoxyloid clade) represents the genera that yield stromatal pigments in potassium hydroxide and produce a nodulisporium-like asexual morph, Clade B (xylarioid clade) represents the genera that do not yield stromatal pigments in potassium hydroxide and produce a geniculosporium-like asexual morph except for Anthostomella,whose species may either have nodulisporium-like or libertella-like asexual morphs.…”

Section: Discussionsupporting

confidence: 75%

A multiple gene genealogy reveals phylogenetic placement of Rhopalostroma lekae

et al. 2014

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Rhopalostroma lekae was collected on bark of wood in Chiang Rai Province, Northern Thailand and isolates from the sexual state produced a nodulosporium-like asexual state in culture. A combined multigene sequence analysis was used to infer the phylogenetic position of R. lekae and its affinities with other xylariaceous genera. Rhopalostroma is confirmed to have particularly close affinities with the genera Phylacia and Thamnomyces. Secondary metabolite profiling of R. lekae showed the species to produce binaphthalene tetrol (BNT) as a major metabolite and several minor undetermined metabolites. The phylogenetic placement of R. lekae was resolved using a polythetic approach. Herbarium material and living cultures representing an authentic specimen of R. lekae are deposited in publically accessible collections that can be used in future studies.

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Section: Discussionsupporting

confidence: 75%

A multiple gene genealogy reveals phylogenetic placement of Rhopalostroma lekae

et al. 2014

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“…The studies by Sánchez-Ballesteros et al (2000), Triebel et al (2005) and Pelaez et al (2008) exclusively used DNA loci such as the internal transcribed spacer region (ITS) of the nuclear ribosomal DNA (rDNA) that is widely accepted by the community as standard barcode of fungi (Schoch et al 2012), but failed to resolve the taxa in the hypoxyloid clades, due to its limitations as phylogenetically informative locus. The studies by Hsieh et al (2005, with focus on Hypoxylon and allies), and Hsieh et al (2010, with focus on the xylarioid clades) provided a better resolution based on the protein-coding genes alpha-actin (ACT) and beta-tubulin (TUB2).…”

Section: Introductionmentioning

confidence: 99%

Resurrection and emendation of the Hypoxylaceae, recognised from a multigene phylogeny of the Xylariales

et al. 2017

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A multigene phylogeny was constructed, including a significant number of representative species of the main lineages in the Xylariaceae and four DNA loci the internal transcribed spacer region (ITS), the large subunit (LSU) of the nuclear rDNA, the second largest subunit of the RNA polymerase II (RPB2), and beta-tubulin (TUB2). Specimens were selected based on more than a decade of intensive morphological and chemotaxonomic work, and cautious taxon sampling was performed to cover the major lineages of the Xylariaceae; however, with emphasis on hypoxyloid species. The comprehensive phylogenetic analysis revealed a clear-cut segregation of the Xylariaceae into several major clades, which was well in accordance with previously established morphological and chemotaxonomic concepts. One of these clades contained Annulohypoxylon, Hypoxylon, Daldinia, and other related genera that have stromatal pigments and a nodulisporium-like anamorph. They are accommodated in the family Hypoxylaceae, which is resurrected and emended. Representatives of genera with a nodulisporium-like anamorph and bipartite stromata, lacking stromatal pigments (i.e. Biscogniauxia, Camillea, and Obolarina) appeared in a clade basal to the xylarioid taxa. As they clustered with Graphostroma platystomum, they are accommodated in the Graphostromataceae. The new genus Jackrogersella with J. multiformis as type species is segregated from Annulohypoxylon. The genus Pyrenopolyporus is resurrected for Hypoxylon polyporus and allied species. The genus Daldinia and its allies Entonaema, Rhopalostroma, Ruwenzoria, and Thamnomyces appeared in two separate subclades, which may warrant further splitting of Daldinia in the future, and even Hypoxylon was divided in several clades. However, more species of these genera need to be studied before a conclusive taxonomic rearrangement can be envisaged. Epitypes were designated for several important species in which living cultures and molecular data are available, in order to stabilise the taxonomy of the Xylariales.

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“…However, in recent years, the molecular approach by using Internal Transcribed Spacer (ITS) region/ 18S rRNA gene sequence are used for identification of mushrooms including other eukaryotes, as these sequences are conserved irrespective of life history and evolution (Rajaratnam and Thiagarajan, 2012). The internal transcribed spacer (ITS) region of the ribosomal DNA (rDNA) has been widely used for identification of mushrooms at both species and genus level (Sanchez-Ballesteros et al, 2000). The ITS region fragment ranges between 450 to 700 base pair has the highest probability of successful identification for the broadest range of fungi, with the most clearly defined gap between inter-and intra-specific variation (Schoch et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Molecular identification and screening of mushrooms for antibacterial property against Pseudomonas aeruginosa and Staphylococcus aureus

Mariselvi

Ninganna

2018

JANS

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In this study, 18 mushrooms were collected from Gandhi Krishi Vigyana Kendra (GKVK) campus, University of Agricultural Sciences, Bangalore (India) and identified by Internal Transcribed Spacer (ITS) region using National Centre for Biotechnology Information (NCBI) data base and screened for antibacterial property against two skin infection causing bacteria viz.,Pseudomonas aeruginosa and Staphylococcus aureus in dogs. Eleven mushrooms extract inhibited both the bacteria showing their antimicrobials as broad spectrum while inhibiting gram positive as well as gram negative bacteria. The highest zone of inhibition was recorded from the extract of Lyophyllum sp. The extract of two mushrooms viz., Phlebopus portentosus and Termitomyces sp. inhibited only P. aeruginosa, a gram negative bacterium and the extract of three mushrooms viz., Agaricus sp, Coprinellus disseminates and Agaricus blazei inhibited only S. aureus, a gram positive bacterium, suggesting spectral specificity of their antimicrobial compounds. However, the extracts of two mushrooms viz.,Macrocybe gigantea and Schizophyllum commune did not show inhibition of any of the pathogens. Hence, this study suggests that the occurrence of potential pharmaceuticals in mushrooms can be exploited for control of multidrug resistant bacteria.

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Phylogenetic Study of Hypoxylon and Related Genera Based on Ribosomal ITS Sequences

Cited by 60 publications

References 0 publications

A multiple gene genealogy reveals phylogenetic placement of Rhopalostroma lekae

A multiple gene genealogy reveals phylogenetic placement of Rhopalostroma lekae

Resurrection and emendation of the Hypoxylaceae, recognised from a multigene phylogeny of the Xylariales

Molecular identification and screening of mushrooms for antibacterial property against Pseudomonas aeruginosa and Staphylococcus aureus

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