Phylogenetic study and molecular identification of 31 Dendrobium species using inter-simple sequence repeat (ISSR) markers

Wang, Huizhong; Feng, Shi Ting; Lu, Jiangjie; Shi, Nongnong; Liu, Junjun

doi:10.1016/j.scienta.2009.06.005

Cited by 81 publications

(61 citation statements)

References 25 publications

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“…Mutations resulting from deletions/duplications or single nucleotide changes at the primer binding sites can be identified. In orchids, ISSRs have been utilized to assess the level of genetic diversity in cultivated spring orchid (Cymbidium goeringii), to estimate genetic stability of micropropagated jewel orchid (Anoectochilus formosanus), and to determine the phylogenetic relationship of Dendrobium species (Wang et al, 2009;Zhang et al, 2010). In this study, we evaluated the effectiveness of ISSRs in detecting and fingerprinting Dendrobium 'Earsakul' mutants induced by in vitro mutagenesis of protocorm-like bodies (PLBs) using different concentrations of NaN 3 , and we assessed the genetic variability of these mutants.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of genetic variability in in vitro sodium azide-induced Dendrobium ‘Earsakul’ mutants

et al. 2014

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ABSTRACT. In vitro mutagenesis of Dendrobium 'Earsakul' was carried out by incubating the protocorm-like bodies in 0-5 mM sodium azide for 1 h. Twenty-eight putative mutants were evaluated for genetic variability compared to untreated control plants using inter-simple sequence repeat (ISSR) analysis. Polymorphic fragments were produced by 9 of 12 ISSR primers. A total of 173 amplified ISSR fragments varying in size from 140 to 5000 bp were obtained, 39 of which were polymorphic (22.5%). Of the 28 putative mutants, 15 (53.6%) showed altered genetic profiles compared to control and were identified as mutants. These results suggest that sodium azide can be effectively utilized to generate mutants in Dendrobium 'Earsakul', and ISSR provides a powerful tool that allows efficient early detection of these mutants. The identified mutants are currently being multiplied for further evaluation of their horticultural characteristics.

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Section: Introductionmentioning

confidence: 99%

Evaluation of genetic variability in in vitro sodium azide-induced Dendrobium ‘Earsakul’ mutants

et al. 2014

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“…The ISSR markers use is effective to analyses genetic diversity in the Cattleya genus, as seen in other orchid genus as Dendrobium, especially due to the lack of total genome information (Wang et al, 2009). The protocol used in this work was very efficient for extracting DNA from Cattleya leafs, even though it was described for stem bark of Leguminosae tree, presenting high polymorphism rates (96.9%).…”

Section: Discussionmentioning

confidence: 99%

“…This polymorphism is justified by the use of the three species and the different accessions of each. High levels of polymorphism were found in other orchids genus such as Dendrobium (Wang, 2009), Piperia (George, 2009) and Cymbidium .…”

Section: Discussionmentioning

confidence: 99%

“…The ISSR markers were useful in phylogenetic relations determination studies among species and subjects within the same species in orchids such as Dendrobium (Wang et al, 2009), Piperiayadonii (George et al, 2009), Cymbidiumspp , Cattleyaspp and Brassavolatuberculata (Fajardo et al, 2014). In this study the ISSR markers were used for the purpose of comparing the polymorphic fragment pattern in commercialized plants named Cattleya guttata, C. tigrina e C. leopoldii, aiming to find variations in the germplasm.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Study on Endemic Cattleya Species from Brazilian Flora

Moraes¹,

Bertão²,

Loose³

et al. 2014

AIJB

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The orchids are known for its beautiful flowers, the genetic recombination ability and the lack of its defined taxonomic classification of the Orchidaceae family. Phylogenetic studies using molecular technics allow additional taxonomy understanding. ISSR molecular markers can determine the phylogenetic relationships among species and individuals of the same species. In this study the patterns of polymorphism were compared in subjects of Cattleya guttata, C. tigrina and C. leopoldii in order to find variations in the germplasm which can contribute to elucidate phylogenetic issues. Results were obtained using 13 primers in ten subjects of each species. They increased 97 loci being 96.9% polymorphic for the three studied species. It is concluded that the protocol was effective for the identification of fragments in all studied species. The analysis of total banding pattern suggests that C. guttata and C. leopoldii are molecularly related to each other and more distant from C. tigrina.

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“…Dendrobium has important economic significance, and some species are very famous ornamental plants (Wang et al, 2009). Moreover, the fresh or dried stems of Dendrobium species have the efficacy of maintaining gastric tonicity, enhancing production of body fluid, nourishing Yin, and antipyresis (Chinese Pharmacopoeia Committee, 2015).…”

Section: Introductionmentioning

confidence: 99%

Authentication of Chinese Herbal Medicines Dendrobium Species and Phylogenetic Study Based on nrDNA ITS Sequence

Wang¹

2018

IJAB

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Dendrobium plants are one of the most valuable traditional Chinese medicinal herbs. Because of excessive exploitation and habitat degradation, Dendrobium species are endangered. Moreover, during no flowering stages, it is very difficult and even impossible to distinguish Dendrobium species based on morphological characteristics. Therefore, exploiting other DNA barcodes as complementary molecular marker for distinguishing Dendrobium spp. are necessary. Hence, developing an uncomplicated and exact method for authenticating Dendrobium spp. is imperative. In this study, nuclear ribosomal DNA internal transcribed spacer (ITS) sequences of 320 samples from 73 Dendrobium spp. were analyzed. The average intra-and inter-specific genetic divergences were 0.007 and 0.220, respectively. The TaxonGap method indicated that between-species variation of about 80.9% of the species were higher than within-species variation. The neighbor-joining tree showed that 73 Dendrobium spp. were divided into four major clades. Phylogenetic relationships between Dendrobium spp. supported traditional morphological methods and previously published molecular data. This study indicated that ITS region was not only suitable for species identification, but also for phylogenetic analysis of Dendrobium. However, it was also found that ITS could not readily resolve all species determination problems in Dendrobium.

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Phylogenetic study and molecular identification of 31 Dendrobium species using inter-simple sequence repeat (ISSR) markers

Cited by 81 publications

References 25 publications

Evaluation of genetic variability in in vitro sodium azide-induced Dendrobium ‘Earsakul’ mutants

Evaluation of genetic variability in in vitro sodium azide-induced Dendrobium ‘Earsakul’ mutants

Molecular Study on Endemic Cattleya Species from Brazilian Flora

Authentication of Chinese Herbal Medicines Dendrobium Species and Phylogenetic Study Based on nrDNA ITS Sequence

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