2019
DOI: 10.1007/978-1-4939-9210-2_2
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Phylogenetic Studies

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Cited by 5 publications
(6 citation statements)
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“…Moreover, the fact that most maxicircle-encoded transcripts require specific addition and deletion of uridine residues by the RNA editing machinery to form translatable mRNAs introduces an additional evolutionary variability among the orthologous genes. Notably, a maxicircle gene, cytochrome b (Cyb; Figure 1 ), is a classic and widely used marker for phylogenetic studies [ 50 , 51 ], but it is known that cytochrome b sequences cannot discriminate species such as L. donovani and L. infantum , or L. braziliensis and L. peruviana [ 10 ].…”
Section: Resultsmentioning
confidence: 99%
“…Moreover, the fact that most maxicircle-encoded transcripts require specific addition and deletion of uridine residues by the RNA editing machinery to form translatable mRNAs introduces an additional evolutionary variability among the orthologous genes. Notably, a maxicircle gene, cytochrome b (Cyb; Figure 1 ), is a classic and widely used marker for phylogenetic studies [ 50 , 51 ], but it is known that cytochrome b sequences cannot discriminate species such as L. donovani and L. infantum , or L. braziliensis and L. peruviana [ 10 ].…”
Section: Resultsmentioning
confidence: 99%
“…In fact, the sequence of the cytochrome b (Cyt b) gene, which is part of the maxicircle, is being used in many studies for phylogeny analysis within the Leishmania genus [63,64]. Moreover, the Cyt b gene is included in many of the multilocus sequence typing (MLST) sets currently used for phylogenetic studies in Leishmania [65]. MLST is based on the use of concatenated sequences from several genes and is aimed to ensure robust phylogenetic inferences.…”
Section: Discussionmentioning
confidence: 99%
“…Many markers and techniques have been used for Leishmania identification, as reviewed (Akhoundi et al., 2017; Kuhls & Mauricio, 2019; Van der Auwera & Dujardin, 2015). Nevertheless, in most studies, they have been applied to a low number of strains, to strains isolated only from the Old or New World, without covering both Leishmania and Viannia subgenera, or are limited to a single technique.…”
Section: Discussionmentioning
confidence: 99%
“…A third approach is sequencing, which generally takes up an intermediate position regarding discriminative power, workability and time consumption, but its pros and cons are hardly influenced by the target used (Kuhls & Mauricio, 2019). In the current study, the four analysed genetic regions permitted a confident identification of the Leishmania subgenus at the species level.…”
Section: Discussionmentioning
confidence: 99%
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