Phylogenetic, structural, and functional characterization of AMT3;1, an ammonium transporter induced by mycorrhization among model grasses

Koegel, Sally; Mieulet, Delphine; Baday, Sefer; Chatagnier, Odile; Lehmann, Moritz F.; Wiemken, Andres; Boller, Thomas; Wipf, Daniel; Bernèche, Simon; Guiderdoni, Emmanuel; Courty, Pierre‐Emmanuel

doi:10.1007/s00572-017-0786-8

Cited by 33 publications

(30 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expression of SbAMT3;1 and SbAMT4 genes in Sorghum bicolor is induced by mycorrhizal formation, and SbAMT3;1 is restricted to cells containing developing arbuscules in the mycorrhizal roots [65]. Knockdown of SbAMT3;1 reduces the rate of ammonium acquisition from symbiotic fungi and arrests the growth promotion effect experienced by plants after fungi inoculation [69]. These results confirm the role of AMTs in importing ammonium nitrogen from the symbiotic fungus to the plant partner.…”

Section: Amt Mediates Ammonium Transport From Symbiotic Fungi To Hostsupporting

confidence: 64%

Function and Regulation of Ammonium Transporters in Plants

Hao

Zhou

Yang

et al. 2020

IJMS

View full text Add to dashboard Cite

Ammonium transporter (AMT)-mediated acquisition of ammonium nitrogen from soils is essential for the nitrogen demand of plants, especially for those plants growing in flooded or acidic soils where ammonium is dominant. Recent advances show that AMTs additionally participate in many other physiological processes such as transporting ammonium from symbiotic fungi to plants, transporting ammonium from roots to shoots, transferring ammonium in leaves and reproductive organs, or facilitating resistance to plant diseases via ammonium transport. Besides being a transporter, several AMTs are required for the root development upon ammonium exposure. To avoid the adverse effects of inadequate or excessive intake of ammonium nitrogen on plant growth and development, activities of AMTs are fine-tuned not only at the transcriptional level by the participation of at least four transcription factors, but also at protein level by phosphorylation, pH, endocytosis, and heterotrimerization. Despite these progresses, it is worth noting that stronger growth inhibition, not facilitation, unfortunately occurs when AMT overexpression lines are exposed to optimal or slightly excessive ammonium. This implies that a long road remains towards overcoming potential limiting factors and achieving AMT-facilitated yield increase to accomplish the goal of persistent yield increase under the present high nitrogen input mode in agriculture.

show abstract

Section: Amt Mediates Ammonium Transport From Symbiotic Fungi To Hostsupporting

confidence: 64%

Function and Regulation of Ammonium Transporters in Plants

Hao

Zhou

Yang

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…To evaluate the success of mycorrhization of the R. irregularis-inoculated rice roots at the molecular level, we determined the expression of six genes that previously were shown to be specifically induced in rice roots during AM symbiosis: OsAM1, OsAM10, OsAM20 and OsAM25 (Güimil et al 2005), the mycorrhiza-specific phosphate transporter OsPT11 (Paszkowski et al 2002) and the ammonium transporter OsAMT3;1 (Perez-Tienda et al 2014;Koegel et al 2017). In all nutrient solutions of both experiments, OsAM1, OsAM10, OsAM20, OsAM25 and OsPT11 were significantly, greatly upregulated in AM roots compared to nonmycorrhizal roots (Supplementary Table S2), thus confirming a well-established rice-mycorrhiza under all five nutritional regimes.…”

Section: Resultsmentioning

confidence: 99%

Identification of arbuscular mycorrhiza-inducible Nitrate Transporter 1/Peptide Transporter Family (NPF) genes in rice

Drechsler¹,

Courty²,

Brulé³

et al. 2017

Mycorrhiza

Self Cite

View full text Add to dashboard Cite

Arbuscular mycorrhizal fungi (AMF) colonize up to 90% of all land plants and facilitate the acquisition of mineral nutrients by their hosts. Inorganic orthophosphate (P i ) and nitrogen (N) are the major nutrients transferred from the fungi to plants. While plant P i transporters involved in nutrient transfer at the plant-fungal interface have been well studied, the plant N transporters participating in this process are largely unknown except for some ammonium transporters (AMT) specifically assigned to arbuscule-colonized cortical cells. In plants, many nitrate transporter 1/peptide transporter family (NPF) members are involved in the translocation of nitrogenous compounds including nitrate, amino acids, peptides and plant hormones. Whether NPF members respond to AMF colonization, however, is not yet known. Here, we investigated the transcriptional regulation of 82 rice (Oryza sativa) NPF genes in response to colonization by the AMF Rhizophagus irregularis in roots of plants grown under five different nutrition regimes. Expression of the four OsNPF genes NPF2.2/ PTR2, NPF1.3, NPF6.4 and NPF4.12 was strongly induced in mycorrhizal roots and depended on the composition of the fertilizer solution, nominating them as interesting candidates for nutrient signaling and exchange processes at the plantfungal interface.

show abstract

Section: Improved Plant Nitrogen Nutrition In Plant–fungus Associatiomentioning

confidence: 99%

“…Following colonization by AMF, plant transcripts of genes coding for NO 3 – and NH 4 + transporters are up-regulated ( Hildebrandt et al , 2002 ; Courty et al , 2015 ; Garcia et al , 2016 ; Koegel et al , 2017 ). Similarly, fungal NO 3 – and NH 4 + transporters are up-regulated during the plant colonization process ( Courty et al , 2015 ; Koegel et al , 2017 ). So far, most of the studies aiming to understand the mechanisms involved in plant N nutrition in the presence of AMF have been based on gene transcriptional activation ( Guether et al , 2009 ; Pérez-Tienda et al , 2014 ; Garcia et al , 2016 ; Chen et al , 2018 b).…”

Section: Improved Plant Nitrogen Nutrition In Plant–fungus Associatiomentioning

confidence: 99%

“…Interestingly, specific up-regulation of some transporters has been observed in roots colonized by AMF, indicating that under these conditions the transport mechanism is modified compared with that found in non-colonized plants ( Garcia et al , 2016 ). For instance, the AMT3.1 plant NH 4 + transporter transcript is specifically up-regulated in cereals such as sorghum, maize, and rice colonized by AMF ( Koegel et al , 2017 ). The same study proposed that AMT3.1 is the major driver of N and phosphorus (P) transfer to the plant colonized by AMF ( Koegel et al , 2017 ).…”

Section: Improved Plant Nitrogen Nutrition In Plant–fungus Associatiomentioning

confidence: 99%

See 1 more Smart Citation

Beneficial soil-borne bacteria and fungi: a promising way to improve plant nitrogen acquisition

Dellagi

Quilleré

Hirel

2020

Journal of Experimental Botany

View full text Add to dashboard Cite

Nitrogen (N) is an essential element for plant productivity, thus, it is abundantly applied to the soil in the form of organic or chemical fertilizers that have negative impacts on the environment. Exploiting the potential of beneficial microbes and identifying crop genotypes that can capitalize on symbiotic associations may be possible ways to significantly reduce the use of N fertilizers. The best-known example of symbiotic association that can reduce the use of N fertilizers is the N2-fixing rhizobial bacteria and legumes. Bacterial taxa other than rhizobial species can develop associative symbiotic interactions with plants and also fix N. These include bacteria of the genera Azospirillum, Azotobacter, and Bacillus, some of which are commercialized as bio-inoculants. Arbuscular mycorrhizal fungi are other microorganisms that can develop symbiotic associations with most terrestrial plants, favoring access to nutrients in a larger soil volume through their extraradical mycelium. Using combinations of different beneficial microbial species is a promising strategy to boost plant N acquisition and foster a synergistic beneficial effect between symbiotic microorganisms. Complex biological mechanisms including molecular, metabolic, and physiological processes dictate the establishment and efficiency of such multipartite symbiotic associations. In this review, we present an overview of the current knowledge and future prospects regarding plant N nutrition improvement through the use of beneficial bacteria and fungi associated with plants, individually or in combination.

show abstract

Phylogenetic, structural, and functional characterization of AMT3;1, an ammonium transporter induced by mycorrhization among model grasses

Cited by 33 publications

References 67 publications

Function and Regulation of Ammonium Transporters in Plants

Function and Regulation of Ammonium Transporters in Plants

Identification of arbuscular mycorrhiza-inducible Nitrate Transporter 1/Peptide Transporter Family (NPF) genes in rice

Beneficial soil-borne bacteria and fungi: a promising way to improve plant nitrogen acquisition

Contact Info

Product

Resources

About