Phylogenetic Sequence Analysis, Recombinant Expression, and Tissue Distribution of a Channel Catfish Estrogen Receptor β

Xia, Zuo‐Li; Gale, William L.; Chang, Xiaotian; Langenau, David M.; Patiño, Reynaldo; Maule, Alec G.; Densmore, Llewellyn D.

doi:10.1006/gcen.1999.7447

Cited by 64 publications

(27 citation statements)

References 17 publications

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“…Moreover, hER but not cfER showed a higher luciferase activity with 10 7 M (77-fold induction) than with 10 9 M (29-fold induction) E 2 . The tissue distribution of the cfER transcript was essentially the same as found for the channel catfish ER (Xia et al 2000). ER expression in the gilthead seabream was only detected in testis, liver and heart (Socorro et al 2000).…”

Section: Discussionsupporting

confidence: 59%

“…The cloning of ER subtypes from several teleost fish species supported the notion that also in nonmammalian species ER and ER subtypes exist (Chang et al 1999, Tchoudakona et al 1999, Hawkins et al 2000, Socorro et al 2000, Xia et al 2000. Detailed phylogenetic analyses of ERs revealed that each receptor subtype contains two major sister clades, piscine (teleost) and tetrapod ERs (Xia et al 2000).…”

Section: Introductionmentioning

confidence: 74%

“…To our surprise we detected very low cfER mRNA levels in the liver of a male African catfish, since most of the teleost ER were first cloned from this tissue. However, also in the liver of male channel catfish very low ER mRNA levels were found, while clear ER mRNA levels were detected in the liver of female channel catfish (Xia et al 2000). Also, the presence of specific ERs in the cytosol of male rainbow trout liver has been reported and has been connected with the regulation of estrogen-dependent production of hepatic vitellogenin (Maitre et al 1985).…”

Section: Discussionmentioning

confidence: 99%

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Cloning and expression of a functional estrogen receptor-alpha from African catfish (Clarias gariepinus) pituitary

Teves

Granneman²,

Dijk³

et al. 2003

Journal of Molecular Endocrinology

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An African catfish (Clarias gariepinus) estrogen receptor-α (cfERα) cDNA fragment was amplified by RT-PCR, in combination with a modified 3′-RACE procedure, on total RNA extracted from pituitary. This cDNA fragment was used to screen an African catfish pituitary cDNA library. A clone was obtained that contained an open-reading frame coding for a 620 amino acid cfERα protein with a deduced molecular mass of 68·1 kDa. In addition, a partial African catfish estrogen receptor-β (cfERβ) cDNA fragment was amplified by RT-PCR on total RNA extracted from testis.Neighbor-joining analysis was used to infer a phylogenetic classification for cfERα and cfERβ. The tree obtained indicated that there are two major clusters of vertebrate ERs: ERα and ERβ. Within each cluster, teleost and tetrapod ER sister clades could be distinguished. The cfERα clustered with other teleost ERαs, whereas cfERβ clustered with other teleost ERβs.The ligand-induced transcriptional activity of cfERα was demonstrated in a transient gene expression assay using cells in which an acute estrogenic response was created by co-transfecting cultures with recombinant cfERα cDNA expression vector constructs in the presence of an estrogen-dependent reporter plasmid.Real-time, quantitative PCR revealed that cfERα transcripts were most abundantly expressed in pituitary, while in all other tissues tested the relative cfERα mRNA levels were less than ∼5% of the level obtained in pituitary. Moreover, we found that, during pubertal development, the relative cfERα mRNA levels gradually increased in African catfish pituitary.

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Section: Discussionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

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Cloning and expression of a functional estrogen receptor-alpha from African catfish (Clarias gariepinus) pituitary

Teves

Granneman²,

Dijk³

et al. 2003

Journal of Molecular Endocrinology

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“…In contrast with these findings, however, esr1 in African catfish (Clarias gariepinus) and esr2a in goldfish (Carassius auratus) have been reported to be expressed predominantly in pituitary and brain, with considerably lower levels of expression in the gonad and liver [46][47][48]. Although differences in the ligand-binding traits of the ER subtypes have been indicated in fish [49][50][51], functional differences, in terms of their roles in reproduction and other physiological processes, have not been established in fish.…”

Section: Introductionmentioning

confidence: 68%

Molecular Characterization of Estrogen Receptors 1, 2a, and 2b and Their Tissue and Ontogenic Expression Profiles in Fathead Minnow (Pimephales promelas)1

Filby

Tyler

2005

Biology of Reproduction

201

145

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There are two estrogen receptor (ER) subtypes in fish, Esr1 and Esr2 (formerly ERalpha and ERbeta), and in some species the Esr2 subtype has two forms, Esr2b (formerly ERbeta1) and Esr2a (formerly ERbeta2 or ERgamma). There is little information, however, on the different characteristics and functional significance of the two receptor subtypes in fish, and this is especially relevant for understanding the disruption of ER signaling by chemicals with estrogenic activity. In this study, the full-length cDNAs for esr1 (3167 base pairs [bp]) and esr2b (2318 bp), and a partial-length (267 bp) cDNA for esr2a, were cloned and characterized in fathead minnow (fhm; Pimephales promelas), and their patterns of expression established during development and in adults. Real-time polymerase chain reaction revealed some clear distinctions in the ontogenic and tissue expression of fhm esr1, esr2b, and esr2a, suggesting different functions for each ER subtype. Fhm ERs were expressed in brain, pituitary, liver, gonad, intestine, and gill of male and female fish, esr2b and esr2a were also expressed in muscle. Fhm esr1 and esr2b were expressed predominantly in the liver, whereas fhm esr2a was expressed predominantly in intestine and was lowest expressed in liver. Responses of the different hepatic ERs in male fathead minnow exposed to 100 ng estradiol/L differed, with a significant induction (5-fold) of fhm esr1 but no effect on esr2b or esr2a expression, suggesting different mechanisms of regulation for the different ERs. The detailed characterization of ERs in fathead minnow provides the foundation for understanding the molecular basis of estrogenic disruption in fish.

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“…In order to determine whether immune tissues are potential targets for estrogens, several studies have looked at the expression of ER in immune tissues. In immature and mature male and female channel catfish, for example, ER is expressed in spleen, blood and head-kidney, while ER is only expressed in spleen (Xia et al, 2000). ER is expressed in the spleen and head-kidney of male and female common solea (Caviola et al, 2007).…”

Section: Sex Steroid Hormones As Regulators Of the Immune Responsementioning

confidence: 99%

Sex Steroids Modulate Fish Immune Response

Chaves-Pozo¹,

Cabas²,

García‐Ayala³

2012

Sex Steroids

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Phylogenetic Sequence Analysis, Recombinant Expression, and Tissue Distribution of a Channel Catfish Estrogen Receptor β

Cited by 64 publications

References 17 publications

Cloning and expression of a functional estrogen receptor-alpha from African catfish (Clarias gariepinus) pituitary

Cloning and expression of a functional estrogen receptor-alpha from African catfish (Clarias gariepinus) pituitary

Molecular Characterization of Estrogen Receptors 1, 2a, and 2b and Their Tissue and Ontogenic Expression Profiles in Fathead Minnow (Pimephales promelas)1

Sex Steroids Modulate Fish Immune Response

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