Phylogenetic Relationships within Terrestrial Mites (Acari: Prostigmata, Parasitengona) Inferred from Comparative DNA Sequence Analysis of the Mitochondrial Cytochrome Oxidase Subunit I Gene

Söller, Rainer; Wohltmann, Andreas; Witte, H.J.L.; Blohm, Dietmar

doi:10.1006/mpev.2000.0855

Cited by 30 publications

(18 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, two different analyses were run for all three genes concatenated, one including all three codon positions of the COI gene and another with the third positions removed. Removing saturated codon positions is a standard practice in phylogenetic analyses and has proven to provide more resolved phylogenetic trees (Chiari et al, 2012;Gleeson et al, 1998;Shanmei Zou and Lingfeng, 2011;Söller et al, 2001). In the present study, the topologies of the trees with and without third codon positions of COI were very similar, but support values for the clades recovered in the tree without the third codon positions were higher in the Bayesian analysis ( Fig.…”

Section: Resultssupporting

confidence: 45%

Phylogeny of the family Aglajidae (Pilsbry, 1895) (Heterobranchia: Cephalaspidea) inferred from mtDNA and nDNA

Camacho-García

Ornelas-Gatdula

Gosliner

et al. 2014

Molecular Phylogenetics and Evolution

View full text Add to dashboard Cite

Section: Resultssupporting

confidence: 45%

Phylogeny of the family Aglajidae (Pilsbry, 1895) (Heterobranchia: Cephalaspidea) inferred from mtDNA and nDNA

Camacho-García

Ornelas-Gatdula

Gosliner

et al. 2014

Molecular Phylogenetics and Evolution

View full text Add to dashboard Cite

“…According to extraction methods already published for various acari (Eriophyidae, Ixodidae, Laelapidae, Phytoseiidae, Tetranychidae, Trombidiidae), we chose and compared three conventional methods for extracting DNA from D. gallinae: a CTAB method, with a lysis step followed by a phenol chloroform precipitation [17,20], a Qiamp DNA extraction kit using separation with a column [8,11], and a Chelex resin [10,14,21,25,28,29]. Another attractive method, the FTA technology, was also tested, and compared to the others.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the inhibitory effect of these samples was overcome, by extracting them a second time with the Qiamp DNA extraction kit, or by using BSA, known for reducing the inhibitory effect of haemoglobin [1]. However, the authors of studies using this Chelex resin did not notice any difficulties but they worked on non hematophagous mites (Tetranychidae and Phytoseiidae) [14,21,25,29]. Some authors have already noted that DNA template extracted by Chelex may not be sufficiently purified for standard PCR amplification [13].…”

Section: Discussionmentioning

confidence: 99%

Comparison of four methods of extracting DNA fromD. gallinae(Acari: Dermanyssidae)

et al. 2006

View full text Add to dashboard Cite

-Dermanyssus gallinae is one of the most serious ectoparasites of poultry and it has been implicated as a vector of several major pathogenic diseases. Molecular detection of such pathogens in mites is crucial and therefore, an important step is the extraction of their DNA from mites. So, we compared four DNA extraction protocols from engorged and unfed individual mites: a conventional method using a Cethyl Trimethyl Ammonium Bromide buffer (CTAB), a Chelex resin, a Qiamp DNA extraction kit and a more recent one filter-based technology (FTA). The DNA samples have been tested for their ability to be amplified by an amplification of a D. gallinae 16S rRNA gene region. The best results were obtained using CTAB and Qiagen methods at the same time with unfed and engorged mites (96% and 100% of amplified samples). FTA produced similar results when using unfed mites but not when processing engorged ones (96% and 70%). Finally, the Chelex method was the least efficient in terms of DNA amplification, especially when applied on engorged individuals (50%). The possible inhibitor role of these Chelex extracted DNA was demonstrated by the means of a PCR control on PUC plasmid. No difference was observed with CTAB, Qiamp DNA extraction kit or FTA methods using DNA extracted one year before. D. gallinae / DNA extraction / engorged mites

show abstract

“…This was the approach taken by Söller et al (2001) who used only the first and second codon positions of a 408bp fragment of COI to investigate the phylogeny of the Parasitengona, however, they found that even after removal of third codon positions considerable homoplasy remained and this lead to an incompletely resolved phylogeny with low bootstrap values. They also had trouble amplifying all of their taxa, presumably due to mutations at the primer site.…”

Section: Coimentioning

confidence: 99%

Molecular markers for the phylogenetics of mites and ticks

Cruickshank

2002

Systematic and Applied Acarology

163

119

View full text Add to dashboard Cite

Molecular methods are becoming increasingly important in systematic acarology. In this review I describe the properties of the ideal molecular marker and compare these with genes that have been used for phylogenetic studies of mites and ticks. The second internal transcribed spacer of the nuclear ribosomal gene cluster (ITS2) and the mitochondrial protein-coding gene cytochrome oxidase I (COI) together provide a powerful tool for phylogenetics at low taxonomic levels. The nuclear ribosomal genes 18S and 28S rDNA are equally powerful tool for phylogenetics at the deepest levels within the Acari. What appear to be lacking are markers that are useful at intermediate levels. The mitochondrial ribosomal genes 12S and 16S rDNA have not lived up to their initial promise. I suggest some nuclear protein-coding genes that may be suitable alternatives. Methods for collection and storage of mites for molecular work, DNA extraction and phylogenetic analysis are also briefly discussed.

show abstract

Phylogenetic Relationships within Terrestrial Mites (Acari: Prostigmata, Parasitengona) Inferred from Comparative DNA Sequence Analysis of the Mitochondrial Cytochrome Oxidase Subunit I Gene

Cited by 30 publications

References 23 publications

Phylogeny of the family Aglajidae (Pilsbry, 1895) (Heterobranchia: Cephalaspidea) inferred from mtDNA and nDNA

Phylogeny of the family Aglajidae (Pilsbry, 1895) (Heterobranchia: Cephalaspidea) inferred from mtDNA and nDNA

Comparison of four methods of extracting DNA fromD. gallinae(Acari: Dermanyssidae)

Molecular markers for the phylogenetics of mites and ticks

Contact Info

Product

Resources

About