Phylogenetic relationships of turfgrasses as revealed by restriction fragment analysis of chloroplast DNA

Yaneshita, Makoto; Ohmura, Takao; Sasakuma, Tetsuo; Ogihara, Yasunari

doi:10.1007/bf00223756

Cited by 25 publications

(12 citation statements)

References 14 publications

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“…The PpEXP1 gene was more closely grouped with two other perennial grass species ( F. pratensis and A. stolonifera ), Triticum aestivum , Hordeum vulgare , and Brachypodium distachyon based on the gene sequences. The topology structure fits well to the phylogenetic tree constructed by whole chlorophyll genome sequence and molecular markers [40], [41].…”

Section: Resultsmentioning

confidence: 52%

Transgenic Tobacco Plants Overexpressing a Grass PpEXP1 Gene Exhibit Enhanced Tolerance to Heat Stress

Shi

et al. 2014

PLoS ONE

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Heat stress is a detrimental abiotic stress limiting the growth of many plant species and is associated with various cellular and physiological damages. Expansins are a family of proteins which are known to play roles in regulating cell wall elongation and expansion, as well as other growth and developmental processes. The in vitro roles of expansins regulating plant heat tolerance are not well understood. The objectives of this study were to isolate and clone an expansin gene in a perennial grass species (Poa pratensis) and to determine whether over-expression of expansin may improve plant heat tolerance. Tobacco (Nicotiana tabacum) was used as the model plant for gene transformation and an expansin gene PpEXP1 from Poa pratensis was cloned. Sequence analysis showed PpEXP1 belonged to α-expansins and was closely related to two expansin genes in other perennial grass species (Festuca pratensis and Agrostis stolonifera) as well as Triticum aestivum, Oryza sativa, and Brachypodium distachyon. Transgenic tobacco plants over-expressing PpEXP1 were generated through Agrobacterium-mediated transformation. Under heat stress (42°C) in growth chambers, transgenic tobacco plants over-expressing the PpEXP1 gene exhibited a less structural damage to cells, lower electrolyte leakage, lower levels of membrane lipid peroxidation, and lower content of hydrogen peroxide, as well as higher chlorophyll content, net photosynthetic rate, relative water content, activity of antioxidant enzyme, and seed germination rates, compared to the wild-type plants. These results demonstrated the positive roles of PpEXP1 in enhancing plant tolerance to heat stress and the possibility of using expansins for genetic modification of cool-season perennial grasses in the development of heat-tolerant germplasm and cultivars.

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Section: Resultsmentioning

confidence: 52%

Transgenic Tobacco Plants Overexpressing a Grass PpEXP1 Gene Exhibit Enhanced Tolerance to Heat Stress

Shi

et al. 2014

PLoS ONE

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“…ZJ, ZM, ZT, ZS, and ZMac indicate Z. joponica, Z. matrella, Z. tenuinfolia, Z. sinica, and Z. macrostachya, respectively, based on the morphological classification. sis based on the established restriction map of cpDNA in Z. japonica (Yaneshita et al, 1993) was different from the classification according to morphological characteristics. The chloroplast genome of accession no.…”

Section: Discussionmentioning

confidence: 80%

“…In order to investigate variations in cpDNAs of zoysiagrasses, ten heterologous clones obtained from wheat cpDNA (Ogihara and Tsunewaki, 1988) were used as probes. These clones cover the entire part of Z. japonica chloroplast genome estimated from the restriction map (Yaneshita et al, 1993).…”

Section: Methodsmentioning

confidence: 99%

Genetic variation and interspecific hybridization among natural populations of zoysiagrasses detected by RFLP analyses of chloroplast and nuclear DNA.

Yaneshita¹,

Nagasawa²,

Engelke

et al. 1997

Genes Genet. Syst.

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Genetic variations among 17 accessions of zoysiagrasses collected from natural populations in Japan were investigated by RFLP analyses of chloroplast DNA (cpDNA) and nuclear DNA. These accessions were classified into five species based on morphological characteristics: Zoysia japonica, Z. matrella, Z. tenuifolia, Z. sinica, and Z. macrostachya. On the basis of eight kinds of RFLPs in cpDNAs detected across accessions, six chloroplast genome types (types A-F) were identified. Although type-A cpDNA was shared by five accessions of japonica and four accessions of matrella, derivative cpDNAs of type A, which each arose by a mutation, were identified in one accession of japonica (type B) and in two accessions of matrella (type C). One accession of japonica which showed spikelets similar to those of shapes macrostachya, contained type-F cpDNA as did sinica and macrostachya. The two accessions of tenuifolia each showed a specific cpDNA type, i.e. types D and E.Genetic relationships among the 17 accessions were investigated by the RFLP analyses of nuclear DNA with 20 genomic and gene probes. A dendrogram constructed with genetic distances calculated from the RFLP patterns indicated four major groups among them. Six accessions of japonica comprised one group, whereas the one accession of japonica possessing the type-F cpDNA was clustered with macrostachya and sinica. Four accessions of matrella with type A cpDNA constituted another group in the dendrogram, showing a closer relationship to the japonica accessions than to the other two accessions of matrella. The remaining two accessions of matrella and tenuifolia accessions were grouped together. These data indicate that zoysiagrasses distributed in Japan harbor highly genetic variations, and that interspecific hybridization has occurred in natural populations.

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“…Charmet et al (1997) used RFLP and cpDNA for phylogenetic analysis of the Festuca-Lolium complex. RFLP probes generated from turf grass species were successfully employed for establishment of phylogenetic relation in turf grasses (Yaneshita et al 1993). Even though RFLP markers are reliable and effective, their use has remained limited as a result of slowness, requirements for large amounts of DNA, and high labor inputs.…”

Section: Introductionmentioning

confidence: 99%

Use of tall fescue EST-SSR markers in phylogenetic analysis of cool-season forage grasses

Mian¹,

Saha²,

Hopkins³

et al. 2005

Genome

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Microsatellites or simple sequence repeats (SSRs) are highly useful molecular markers for plant improvement. Expressed sequence tag (EST)-SSR markers have a higher rate of transferability across species than genomic SSR markers and are thus well suited for application in cross-species phylogenetic studies. Our objectives were to examine the amplification of tall fescue EST-SSR markers in 12 grass species representing 8 genera of 4 tribes from 2 subfamilies of Poaceae and the applicability of these markers for phylogenetic analysis of grass species. About 43% of the 145 EST-SSR primer pairs produced PCR bands in all 12 grass species and had high levels of polymorphism in all forage grasses studied. Thus, these markers will be useful in a variety of forage grass species, including the ones tested in this study. SSR marker data were useful in grouping genotypes within each species. Lolium temulentum, a potential model species for cool-season forage grasses, showed a close relation with the major Festuca-Lolium species in the study. Tall wheat grass was found to be closely related to hexaploid wheat, thereby confirming the known taxonomic relations between these species. While clustering of closely related species was found, the effectiveness of such data in evaluating distantly related species needs further investigations. The phylogenetic trees based on DNA sequences of selected SSR bands were in agreement with the phylogenetic relations based on length polymorphism of SSRs markers. Tall fescue EST-SSR markers depicted phylogenetic relations among a wide range of cool-season forage grass species and thus are an important resource for researchers working with such grass species.

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Phylogenetic relationships of turfgrasses as revealed by restriction fragment analysis of chloroplast DNA

Cited by 25 publications

References 14 publications

Transgenic Tobacco Plants Overexpressing a Grass PpEXP1 Gene Exhibit Enhanced Tolerance to Heat Stress

Transgenic Tobacco Plants Overexpressing a Grass PpEXP1 Gene Exhibit Enhanced Tolerance to Heat Stress

Genetic variation and interspecific hybridization among natural populations of zoysiagrasses detected by RFLP analyses of chloroplast and nuclear DNA.

Use of tall fescue EST-SSR markers in phylogenetic analysis of cool-season forage grasses

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