Phylogenetic relationships of<i>Phytophthora andina</i>, a new species from the highlands of Ecuador that is closely related to the Irish potato famine pathogen<i>Phytophthora infestans</i>

Gómez-Alpízar, Luis; Hu, Chia-Hui; Oliva, Ricardo; Forbes, Gregory A.; Ristaino, Jean Beagle

doi:10.3852/07-074r1

Cited by 37 publications

(50 citation statements)

References 54 publications

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“…uniformis and P. alni subsp. multiformis [31]–[33], P. andina with P. infestans as one parent [34]–[36], P. × serendipita ( P. cactorum × P. hedraiandra ) [29], [37], four hybrids in subclade 6b: P. amnicola × P. taxon PgChlamydo (A-PG), P. taxon PgChlamydo× P. amnicola (PG-A), P. thermophila × P. amnicola (T-A), and P. thermophila × P. taxon PgChlamydo (T-PG) [38], as well as three hybrids in subclade 8b: P. porri × P. taxon parsley, P. porri ×a P. primulae -like species, and a third hybrid with two unknown species as parents [39]. It is interesting to note that parents of most individual hybrids belong to the same Phytophthora clade.…”

Section: Introductionmentioning

confidence: 99%

Phytophthora ×stagnum nothosp. nov., a New Hybrid from Irrigation Reservoirs at Ornamental Plant Nurseries in Virginia

2014

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A novel Phytophthora species was frequently recovered from irrigation reservoirs at several ornamental plant production facilities in eastern Virginia. Initial sequencing of the internal transcribed spacer (ITS) region of this species generated unreadable sequences due to continual polymorphic positions. Cloning and sequencing the ITS region as well as sequencing the mitochondrially encoded cytochrome c oxidase 1 and beta-tubulin genes revealed that it is a hybrid between P. taxon PgChlamydo as its paternal parent and an unknown species genetically close to P. mississippiae as its maternal parent. This hybrid has some diagnostic morphological features of P. taxon PgChlamydo and P. mississippiae. It produces catenulate hyphal swellings, characteristic of P. mississippiae, and chlamydospores, typical of P. taxon PgChlamydo. It also produces both ornamented and relatively smooth-walled oogonia. Ornamented oogonia are another important diagnostic character of P. mississippiae. The relatively smooth-walled oogonia may be indicative of oogonial character of P. taxon PgChlamydo. The new hybrid is described here as Phytophthora ×stagnum.

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Section: Introductionmentioning

confidence: 99%

Phytophthora ×stagnum nothosp. nov., a New Hybrid from Irrigation Reservoirs at Ornamental Plant Nurseries in Virginia

2014

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“…Alleles and mitochondrial haplotypes of this population had not been previously reported for other worldwide populations, which supports this explanation. EC-1 clonal lineage coexists with EC-3 in the same region and are the dominating populations on crops of Solanum tuberosum and Solanum lycopersicum (Gomez-Alpizar et al, 2008, Oliva et al, 2010Cardenas et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Foliar virulence of isolates of Phytophthora infestans sensu lato on detached leaves of two Solanum betaceum cultivars

Revelo

Dorado

Lagos

et al. 2011

Trop. plant pathol.

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Foliar virulence of Phytophthora infestans isolates to two Solanum betaceum cultivars, Red and Yellow, was characterized using a detached leaf assay. Six P. infestans sensu lato isolates from S. betaceum belonging to at least two different populations were included (representing the EC-3 clonal lineage and a population that was not previously reported). Three isolates from potato (representing the EC-1 clonal lineage) were included in order to determine their ability to attack S. betaceum cultivars and whether there was difference in virulence between these and isolates from S. betaceum. Significant variation for virulence parameters was found between isolates, isolateorigins, and cultivars. Infection frequency, sporulation intensity, and incubation period allowed differentiation of EC-1 isolates from S. betaceum isolates. Isolates infecting both cultivars exhibited significantly higher values of infection frequency and sporulation intensity and lower latent period values. Cv. Red showed significantly higher values for area under the lesion expansion curve (AULEC), and final lesion size (FLS) and lower values for incubation period (IP) in comparison with cv. Yellow. These results demonstrated that there are two separate populations of P. infestans attacking S. betaceum each with specific virulence towards two different cultivars of Solanum betaceum. Key words: detached leaves assay, EC-1 clonal lineage, EC-3 clonal lineage, late blight, tree tomato. RESUMO Virulência foliar de isolados de Phytophthora infestans sensu lato em folhas destacadas de duas cultivares de Solanum betaceumA agressividade dos isolados de Phytophthora infestans sensu lato obtidos de duas cultivares de Solanum betaceum, Vermelha e Amarela, foi caracterizada em bioensio com folhas destacadas. Seis isolados de S. betaceum pertecendo a pelo menos duas populações diferentes foram incluídos (representando a linhagem clone EC-3 e uma população ainda não reportada). Três isolados obtidos de batata também foram incluídos para determinar se estes poderiam atacar cultivares de S. betaceum e se haveria diferença na agressividade entre isolados, origem dos isolados e cultivares. Freqüência de infecção, densidade populacional e o período de incubação permitiram a diferenciação do isolado EC-1 a partir dos isolados de S. betaceum. Isolados capazes de infectar os dois cultivares exibiram maior frequencia de infecção e densidade populacional e o menor período de latência. Um dos três isolados da população EC-1 não infectou folhas de S. betaceum cv. Vermelha, apresentando o maior valor de freqüência de infecção, maior área abaixo da curva de progresso da expansão de lesão (AACPEL) e tamanho final da lesão e o menor período de incubação. Estes resultados demonstraram a existência de duas populações distintas de P. infestans atacando S. betaceum com agressividades especificas para cada cultivar de Solanum betaceum. Palavras-chave: agressividade foliar, bioensaio com folhas destacadas, linhagem clone EC-1, linhagem clone EC-3, requeima, tamarillo.

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“…However, in some cases, species-specific primers based on the ITS sequences are unable to differentiate closely related species. Gomez-Alpizar et al [24] and Kroon et al [25] reported that P. infestans , P. phaseoli , P. ipomoeae , P . sp.…”

Section: Resultsmentioning

confidence: 99%

Development of SCAR Markers for the Identification ofPhytophthora katsuraeCausing Chestnut Ink Disease in Korea

Lee

et al. 2013

Mycobiology

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Sequence characterized amplified region (SCAR) markers are one of the most effective and accurate tools for microbial identification. In this study, we applied SCAR markers for the rapid and accurate detection of Phytophthora katsurae, the casual agent of chestnut ink disease in Korea. In this study, we developed seven SCAR markers specific to P. katsurae using random amplified polymorphic DNA (RAPD), and assessed the potential of the SCAR markers to serve as tools for identifying P. katsurae. Seven primer pairs (SOPC 1F/SOPC 1R, SOPC 1-1F/SOPC 1-1R, SOPC 3F/SOPC 3R, SOPC 4F/SOPC 4R, SOPC 4F/SOPC 4-1R, SOPD 9F/SOPD 9R, and SOPD 10F/SOPD 10R) from a sequence derived from RAPD fragments were designed for the analysis of the SCAR markers. To evaluate the specificity and sensitivity of the SCAR markers, the genomic DNA of P. katsurae was serially diluted 10-fold to final concentrations from 1 mg/mL to 1 pg/mL. The limit of detection using the SCAR markers ranged from 100 µg/mL to 100 ng/mL. To identify the limit for detecting P. katsurae zoospores, each suspension of zoospores was serially diluted 10-fold to final concentrations from 10 × 105 to 10 × 101 zoospores/mL, and then extracted. The limit of detection by SCAR markers was approximately 10 × 101 zoospores/mL. PCR detection with SCAR markers was specific for P. katsurae, and did not produce any P. katsurae-specific PCR amplicons from 16 other Phytophthora species used as controls. This study shows that SCAR markers are a useful tool for the rapid and effective detection of P. katsurae.

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Phylogenetic relationships ofPhytophthora andina, a new species from the highlands of Ecuador that is closely related to the Irish potato famine pathogenPhytophthora infestans

Cited by 37 publications

References 54 publications

Phytophthora ×stagnum nothosp. nov., a New Hybrid from Irrigation Reservoirs at Ornamental Plant Nurseries in Virginia

Phytophthora ×stagnum nothosp. nov., a New Hybrid from Irrigation Reservoirs at Ornamental Plant Nurseries in Virginia

Foliar virulence of isolates of Phytophthora infestans sensu lato on detached leaves of two Solanum betaceum cultivars

Development of SCAR Markers for the Identification ofPhytophthora katsuraeCausing Chestnut Ink Disease in Korea

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