Phylogenetic relationships in the wheat-aegilops alliance through the prism of the chloroplast genome

Abstract

The nucleotide sequence of the chloroplast genome of the diploid wheat Triticum sinskajae A. Filat. et Kurk. was determined. The comparison of the assembled genome with the chloroplast genomes of some di- and polyploid species of the wheat-aegilops alliance revealed a high evolutionary conservativeness and proximity to the plastid genome of T. monococcum, while the other diploid wheat T. urartu is somewhat distant from them. Alignment of the nucleotide sequences of complete chloroplast genomes of diploid wheat with those of diploid aegilops from the Sitopsis and Vertebrata sections, as well as with tetra - and hexaploid wheat from the turgidum-aestivum and timopheevii groups, allowed to establish that different aegilops from the subsection Truncata, close to the current Aegilops speltoides, were as maternal forms during their formation but not directly Ae.speltoides.

Sequencing and analysis of complete plastid genomes of diploid wheats Tritcum sinskajae and Triticum monococcum k-20970

“…sinskajae is a little-studied diploid wheat, which was previously thought to be formed as a result of a spontaneous mutation of T. monococcum (Filatenko and Kurkiev 2000). Previously, using classical molecular genetic analysis (Kuluev et al 2018), as well as by comparing the nucleotide sequences of plastid genomes (Kuluev et al 2020), we obtained data indicating the existence of a separate species of diploid wheat -T. sinskajae. On the other hand, Sinskaya wheat exhibits complete genetic compatibility with T. monococcum and T. boeoticum (Kurkiev and Filatenko 2000).…”

“…It should be noted that other types of mutations were not detected in the plastid genomes of these Aegilops species. Despite the fact that there are very few nucleotide substitutions in these Aegilops species and the homology of the nucleotide sequences of their chloroplast genomes ranges from 99.98 to 99.99%, they are considered separate species (Kuluev et al 2020, Miki et al 2019. Therefore, the 10 differences we identi ed between the plastid genomes of T. sinskajae and T. monococcum k-20970 allows us to also talk about the species status for Sinskaya wheat.…”

“…monococcum (LC005977.1) using the BLAST resource showed 99.96% similarity between these species. The total number of nucleotide differences was 48, which allowed us to once again raise the question of the species status of T. sinskajae (Kuluev et al 2020). In this study, we set the task of comparing the complete chloroplast genomes of T. sinskajae and the same line k-20970 of T. monococcum, in the samples of which Sinskaya wheat was rst found.…”