Phylogenetic Relationships between Phlebiopsis gigantea and Selected Basidiomycota Species Inferred from Partial DNA Sequence of Elongation Factor 1-Alpha Gene

Wit, Marcin; Sierota, Z.; Żółciak, Anna; Mirzwa-Mróz, Ewa; Jabłońska, Emilia; Wakuliński, W

doi:10.3390/f11050592

Cited by 2 publications

(2 citation statements)

References 37 publications

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“…In Clade A, a subclade A1 included two sequences from the US East coast (SCNC and MWV24089A) and one from the UK (GenBank: KU886025). We note, though, that sequence KU886024 from Poland fell in the Clade B, and the same was observed for all other sequences from the UK, that, although unavailable in GenBank, are reported in Wit et al [76], questioning the actual phylogenetic positioning or validity of the UK sequence KU886025. The phylogenetic analysis of the TEF1-α region identified two main genetic intraspecific A and B lineages, with average within-group distances of 0.02% and 0.11%, respectively.…”

Section: Translation Elongation Factor 1-alpha (Tef1-α)supporting

confidence: 71%

Inter- and Intra-Continental Genetic Variation in the Generalist Conifer Wood Saprobic Fungus Phlebiopsis gigantea

Dovana

Gonthier

Garbelotto

2021

Forests

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Phlebiopsis gigantea (Fr.) Jülich is a well-known generalist conifer wood saprobe and a biocontrol fungus used in several world countries to prevent stump infection by tree pathogenic Heterobasidion fungal species. Previous studies have reported the presence of regional and continental genetic differentiation in host-specific fungi, but the presence of such differentiation for generalist wood saprobes such as P. gigantea has not been often studied or demonstrated. Additionally, little information exists on the distribution of this fungus in western North America. The main purposes of this study were: (I) to assess the presence of P. gigantea in California, (II) to explore the genetic variability of P. gigantea at the intra and inter-continental levels and (III) to analyze the phylogeographic relationships between American and European populations. Seven loci (nrITS, ML5–ML6, ATP6, RPB1, RPB2, GPD and TEF1-α) from 26 isolates of P. gigantea from coniferous forests in diverse geographic distribution and from different hosts were analyzed in this study together with 45 GenBank sequences. One hundred seventy-four new sequences were generated using either universal or specific primers designed in this study. The mitochondrial ML5–ML6 DNA and ATP6 regions were highly conserved and did not show differences between any of the isolates. Conversely, DNA sequences from the ITS, RPB1, RPB2, GPD and TEF1-α loci were variable among samples. Maximum likelihood analysis of GPD and TEF1-α strongly supported the presences of two different subgroups within the species but without congruence or geographic partition, suggesting the presence of retained ancestral polymorphisms. RPB1 and RPB2 sequences separated European isolates from American ones, while the GPD locus separated western North American samples from eastern North American ones. This study reports the presence of P. gigantea in California for the first time using DNA-based confirmation and identifies two older genetically distinct subspecific groups, as well as three genetically differentiated lineages within the species: one from Europe, one from eastern North America and one from California, with the latter presumably including individuals from the rest of western North America. The genetic differentiation identified here among P. gigantea individuals from coniferous forests from different world regions indicates that European isolates of this fungus should not be used in North America (or vice versa), and, likewise, commercially available eastern North American P. gigantea isolates should not be used in western North America forests. The reported lack of host specificity of P. gigantea was documented by the field survey and further reinforces the need to only use local isolates of this biocontrol fungus, given that genetically distinct exotic genotypes of a broad generalist microbe may easily spread and permanently alter the microbial biodiversity of native forest ecosystems.

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Section: Translation Elongation Factor 1-alpha (Tef1-α)supporting

confidence: 71%

Inter- and Intra-Continental Genetic Variation in the Generalist Conifer Wood Saprobic Fungus Phlebiopsis gigantea

Dovana

Gonthier

Garbelotto

2021

Forests

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“…Bref. Wit et al [29] utilized elongation factor 1-alpha (EF1α) partial DNA sequencing and in silico data to infer the relationships between representative isolates of P. gigantea and selected Basidiomycota species. The results obtained indicated that the EF1α region is a valid marker for selecting the most competitive isolates of P. gigantea to be possibly employed for limiting damage by forest tree root pathogens.…”

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confidence: 99%

Recent Advances in the Monitoring, Assessment and Management of Forest Pathogens and Pests

Moricca

Panzavolta

2021

Forests

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Tree pathogens and pests are fundamental components of forest ecosystems. By killing and decomposing susceptible trees, they regulate the cycle of nutrients and energy flow, thus shaping the structure and composition of forest stands. However, ecosystems can be seriously disrupted when the population density of these parasites increases beyond their tolerance level. Ascertaining the origin of pathogen and pest outbreaks, recognizing their causal agents in a precise and unequivocal way, while understanding their reproductive and dispersive dynamics are all crucial for the implementation of effective control measures. The studies collected in this special issue cover a wide range of topics in the field of forest pathology and entomology. Investigations range from molecular diagnosis of pathogens and pests to their monitoring and quantification in the field, from measurements of their proliferation rate to the analysis of their genetic variability, from the assessment of the role of plant diversity and ecosystem heterogeneity on pathogen and pest impacts to disease and pest management. Specific case studies show how applied research conducted with innovative methods is key to solving taxonomic issues that were, until now, controversial. The variety of experimental approaches and the range of scientific issues addressed document the trends and topicality of modern forest health protection science.

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Phylogenetic Relationships between Phlebiopsis gigantea and Selected Basidiomycota Species Inferred from Partial DNA Sequence of Elongation Factor 1-Alpha Gene

Cited by 2 publications

References 37 publications

Inter- and Intra-Continental Genetic Variation in the Generalist Conifer Wood Saprobic Fungus Phlebiopsis gigantea

Inter- and Intra-Continental Genetic Variation in the Generalist Conifer Wood Saprobic Fungus Phlebiopsis gigantea

Recent Advances in the Monitoring, Assessment and Management of Forest Pathogens and Pests

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