Phylogenetic relationships between Leymus and related diploid Triticeae species revealed by ISSR markers

Guo, Guo-Ye; Yang, Ruiwu; Ding, Chunbang; Zhang, Li; Zhou, Yong‐Hong; Chen, Fang; Wang, Shenghua

doi:10.2478/s11756-014-0395-3

Cited by 4 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most important point is that 42 markers amplified the same specific regions in Ns-genome-containing species. Our findings, which provide further evidence that the genera Leymus and Psathyrostachys are closely related and that the Ns genome of Leymus originated from Psathyrostachys , are in accordance with previous inferences [ 46 , 64 ]. Interestingly, marker validation analyses suggested that marker amplification frequencies were considerably higher in Ns and NsXm genomic species than in other wheat-related species involving A, D, E b , E e , V, H, P, R, and St genomes, which reflects the fact that the Ns genome and other genomes are genetically distant from one another.…”

Section: Discussionsupporting

confidence: 93%

Cytogenetic and Molecular Marker Analyses of a Novel Wheat–Psathyrostachys huashanica 7Ns Disomic Addition Line with Powdery Mildew Resistance

Tan

Wang

et al. 2022

IJMS

View full text Add to dashboard Cite

Powdery mildew caused by Blumeria graminis f. sp. tritici is a devastating disease that reduces wheat yield and quality worldwide. The exploration and utilization of new resistance genes from wild wheat relatives is the most effective strategy against this disease. Psathyrostachys huashanica Keng f. ex P. C. Kuo (2n = 2x = 14, NsNs) is an important tertiary gene donor with multiple valuable traits for wheat genetic improvement, especially disease resistance. In this study, we developed and identified a new wheat—P. huashanica disomic addition line, 18-1-5—derived from a cross between P. huashanica and common wheat lines Chinese Spring and CSph2b. Sequential genomic and multicolor fluorescence in situ hybridization analyses revealed that 18-1-5 harbored 21 pairs of wheat chromosomes plus a pair of alien Ns chromosomes. Non-denaturing fluorescence in situ hybridization and molecular marker analyses further demonstrated that the alien chromosomes were derived from chromosome 7Ns of P. huashanica. The assessment of powdery mildew response revealed that line 18-1-5 was highly resistant at the adult stage to powdery mildew pathogens prevalent in China. The evaluation of agronomic traits indicated that 18-1-5 had a significantly reduced plant height and an increased kernel length compared with its wheat parents. Using genotyping-by-sequencing technology, we developed 118 PCR-based markers specifically for chromosome 7Ns of P. huashanica and found that 26 of these markers could be used to distinguish the genomes of P. huashanica and other wheat-related species. Line 18-1-5 can therefore serve as a promising bridging parent for wheat disease resistance breeding. These markers should be conducive for the rapid, precise detection of P. huashanica chromosomes and chromosomal segments carrying Pm resistance gene(s) during marker-assisted breeding and for the investigation of genetic differences and phylogenetic relationships among diverse Ns genomes and other closely related ones.

show abstract

Section: Discussionsupporting

confidence: 93%

Cytogenetic and Molecular Marker Analyses of a Novel Wheat–Psathyrostachys huashanica 7Ns Disomic Addition Line with Powdery Mildew Resistance

Tan

Wang

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…What needs more attention is that the phylogenetic tree based on cp genome sequences existed with minor differences from the phylogenetic tree based on shared protein-coding genes. We inferred that the reason for this is barcode genes laid in the cp genome, and the result is consistent with Liu’s and Guo’s research [ 11 , 16 ]. What is more, the close relationship between Elymus and Leymus may also be related to their origin of genome [ 11 ].…”

Section: Discussionsupporting

confidence: 91%

“…Malacurus (Nevski) Tzvelev [ 7 ]. It has been confirmed that single-copy genes, the mitochondrial coxII intron, molecular markers containing AFLP, RAPD, and ISSR, as well as chloroplast intergenic spacers, are useful tools for Leymus species identification and these previous studies provide important evidence for the subdivision [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ]; however, its complex evolutionary history remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Comparative and Phylogenetic Analysis of Complete Chloroplast Genomes in Leymus (Triticodae, Poaceae)

Tian

Yang

et al. 2022

Genes

View full text Add to dashboard Cite

Leymus is a perennial genus that belongs to the tribe Triticeae (Poaceae) which has an adaptive capacity to ecological conditions and strong resistance to cold, drought, and salinity. Most Leymus species are fine herbs that can be used for agriculture, conservation, and landscaping. Due to confusion taxonomy within genera, the complete chloroplast (cp) genome of 13 Leymus species was sequenced, assembled, and compared with those of three other previously published Leymus species (Leymus condensatus, Leymus angustus, and Leymus mollis) to clarify the issue. Overall, the whole cp genome size ranged between 135,057 (L. condensatus) and 136,906 bp (Leymus coreanus) and showed a typical quadripartite structure. All studied species had 129 genes, including 83 protein-coding genes, 38 transfer RNAs, and 8 ribosomal RNAs. In total, 800 tandem repeats and 707 SSR loci were detected, most of which were distributed in the large single-copy region, followed by the inverted repeat (IR) and small single-copy regions. The sequence identity of all sequences was highly similar, especially concerning the protein-coding and IR regions; in particular, the protein-coding regions were significantly similar to those in the IR regions, regardless of small sequence differences in the whole cp genome. Moreover, the coding regions were more conserved than the non-coding regions. Comparisons of the IR boundaries showed that IR contraction and expansion events were reflected in different locations of rpl22, rps19, ndhH, and psbA genes. The close phylogenetic relationship of Leymus and Psathyrostachys indicated that Psathyrostachys possibly is the donor of the Ns genome sequence identified in Leymus. Altogether, the complete cp genome sequence of Leymus will lay a solid foundation for future population genetics and phylogeography studies, as well as for the analysis of the evolution of economically valuable plants.

show abstract

“…"However, which species of Psathyrostachys potentially served as the genome donor of the Ns genome in Leymus has been identified. The Xm genome is one of the important basic genomes in the perennial Triticeae species" [13,14]. "However, the origin of the Xm genome is still unknown.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Relationships and Diversity in Leymus (Poaceae, Triticeae) Based on Simple Sequence Repeats Markers

Zhang

Lei

Yang

2023

AJOB

View full text Add to dashboard Cite

Aims: The objective of the study was to investigate: (1) the Ns genome donor and elucidate the origins of the Xm genome of Leymus; (2) evaluate the phylogenetic relationships among these species. Methodology: The CTAB (cetyltrimethylammonium bromide) procedure was used to extract total genomic DNA from fresh leaf tissue. A total of 150 pairs of SSR primers were tested to screen those produced polymorphic DNA bands to continue further analysis with Elymus species and 13 diploid perennial species as templates. The GS was used to construct a dendrogram via the unweighted pair group method with arithmetic average (UPGMA) and the SHAN (sequential, hierarchical, agglomerative, and nested clustering) routine in the NTSYS-pc program. Results: The primers WMC475, WMC11 and QWM213 showed more expansion efficiency in the research. There were significant diversity and polymorphism between Leymus and related diploid Triticeae species based on SSR makers. The largest GS coefficient values between Pse. stipifolia and Elymus hystrix indicates that the genetic distance is the closest and has a closer genetic relationship. In clade I, the Leymus species formed a very wellsupported into a small distinct group (Ia) first. Specifically, L. racemosus, L. salinus, L. secalinus and L. triticoides were clustered closely. Conclusion: Psathyrostachys juncea, Psa. huanshanica and Psa. fragilis (Ns genome) clustered together into clade I with Leymus species, which further illustrates Leymus that contains Ns genome is more closely related to Psathyrostachys. St genome and J genome did not participate in the origin of Leymus, and the genetic relationship and genetic distance of Leymus species are related to geographical distribution and environment.

show abstract

Phylogenetic relationships between Leymus and related diploid Triticeae species revealed by ISSR markers

Cited by 4 publications

References 25 publications

Cytogenetic and Molecular Marker Analyses of a Novel Wheat–Psathyrostachys huashanica 7Ns Disomic Addition Line with Powdery Mildew Resistance

Cytogenetic and Molecular Marker Analyses of a Novel Wheat–Psathyrostachys huashanica 7Ns Disomic Addition Line with Powdery Mildew Resistance

Comparative and Phylogenetic Analysis of Complete Chloroplast Genomes in Leymus (Triticodae, Poaceae)

Phylogenetic Relationships and Diversity in Leymus (Poaceae, Triticeae) Based on Simple Sequence Repeats Markers

Contact Info

Product

Resources

About