Phylogenetic relationships and generic reassessment of Proustia and allies (Compositae: Nassauvieae)

Abstract

Proustia is a small southern Andean genus of shrubs, vines and small trees, which are characteristic elements of Chilean and Argentine Andean forests, thickets and desert scrubs. Since Proustia possesses an unusual and characteristic morphology within the Nassauvieae, its circumscription as well as its phylogenetic placement is decisive in understanding the evolution of the tribe. Berylsimpsonia, from the Caribbean, was segregated from Proustia, which currently only includes three species. Lophopappus, another Andean genus, has been closely related to Proustia in sharing style and corolla features that are unusual in the Nassauvieae. The purpose of this work is to evaluate the placement of Proustia within the Nassauvieae, its relationships with Berylsimpsonia and Lophopappus, and the relationships of its species using molecular data and phylogenetic methods. This is the first comprehensive analysis of Proustia. We have included in our study the three currently accepted species of Proustia and seven of its ten infraspecific taxa. For each taxon of Proustia, one to seven accessions were analyzed. Species of Lophopappus and Berylsimpsonia, as well as 16 species belonging to 13 genera of Nassauvieae were also analyzed. We sequenced the nuclear ribosomal ITS and ETS, and the plastid regions ndhF, 5’ trnK–matK, trnL–trnF, and trnL(UAG)–rpl32. Unlike other previous studies, Proustia is not sister to the rest but nested in the tribe in our analyses. Berylsimpsonia vanillosma and P. ilicifolia are distantly related to P. pyrifolia, the type of the genus. According to nuclear DNA data, the infraspecific taxa of P. cuneifolia were recovered all in a well–supported clade, although based on the plastid data P. pyrifolia does not form a lineage separate from P. cuneifolia. Lophopappus is the genus most closely related to Proustia. Regarding the evolution of styles, our results suggest multiple origins of atypical stylar features in the Nassauvieae. As a result of our analyses we propose the segregation of Proustia ilicifolia into the new genus Spinoliva. Morphological data are consistent with the segregation of Proustia and Lophopappus as separate genera. Two species of Proustia, P. cuneifolia and P. pyrifolia are recognized. Three lectotypifications, one neotypification, two new combinations and five rank changes are proposed.

The two South American dry diagonals

“…Kelt et al (2000) showed high similarity of small mammal faunas within the wSADD, though no species were shared between the geographically extreme areas of the Atacama Desert and Patagonia, perhaps because the Andes act as a geographical barrier within the wSADD (see Ab'Sáber 1977, Luebert andWeigend 2014). While most of the endemisms are restricted to portions of it, several plant groups that occupy the entire wSADD are also present in other neighboring regions, such as Ephedra, (Ephedraceae; Ickert-Bond et al 2009), Leucheria (Asteraceae; Jara-Arancio et al 2017), Montiopsis (Montiaceae; Hershkovitz 2019), Nassauvia (Asteraceae; Grossi et al 2017) and Proustia (Asteraceae; Sancho et al 2018). Unlike the eSADD, it is questionable that the wSADD has acted as a corridor for arid adapted taxa.…”