Phylogenetic relationship of Chlorella and Parachlorella gen. nov. (Chlorophyta, Trebouxiophyceae)

Krienitz, Lothar; Hegewald, E.; Hepperle, Dominik; Huss, Volker A. R.; Rohr, Thomas; Wolf, Matthias

doi:10.2216/i0031-8884-43-5-529.1

Cited by 214 publications

(244 citation statements)

References 24 publications

Supporting

Mentioning

220

Contrasting

Unclassified

Order By: Relevance

“…were accepted as genus level divergence among isolates in taxonomic revisions or recent species descriptions (Henley et al 2004, Krienitz et al 2004, Somogyi et al 2011.…”

Section: Community Compositionmentioning

confidence: 99%

Unique picoeukaryotic algal community under multiple environmental stress conditions in a shallow, alkaline pan

et al. 2013

View full text Add to dashboard Cite

Winter phytoplankton communities in the shallow alkaline pans of Hungary are frequently dominated by picoeukaryotes, sometimes in particularly high abundance. In winter 2012 the ice-covered alkaline Zab-szék pan was found to be extraordinarily rich in picoeukaryotic green algae (42-82 × 10 6 cells ml -1 ) despite the simultaneous presence of multiple stressors (low temperature and light intensity with high pH and salinity). The maximum photosynthetic rate of the picoeukaryote community was 1.4 µg C µg chlorophyll a -1 h -1 at 125 µmol m -2 s -1 .The assimilation rates compared with the available light intensity measured on the field show that the community was considerably light-limited. Estimated areal primary production was 2 180 mg C m -2 d -1 . On the basis of the 18S rRNA gene analysis (cloning and DGGE), the community was phylogenetically heterogeneous with several previously undescribed chlorophyte lineages, which indicates the ability of picoeukaryotic communities to maintain high genetic diversity under extreme conditions.

show abstract

“…were accepted as genus level divergence among isolates in taxonomic revisions or recent species descriptions (Henley et al 2004, Krienitz et al 2004, Somogyi et al 2011.…”

Section: Community Compositionmentioning

confidence: 99%

Unique picoeukaryotic algal community under multiple environmental stress conditions in a shallow, alkaline pan

et al. 2013

View full text Add to dashboard Cite

show abstract

“…The final alignments are available in fasta format (see Supplementary Information 3). Species belonging to the Parachlorella clade were used as the outgroup (Krienitz et al, 2004). Evolutionary analyses were conducted in MEGA5 (Tamura et al, 2011).…”

Section: Molecular Analyses and Genbank Accession Numbersmentioning

confidence: 99%

“…However, it is not known whether phylogeny can constrain the evolution of endosymbiosis by restricting the phenotypes generated in the lineage. For example, free-living Micractinium (of the Chlorella clade) forms bristles on its cell wall (Krienitz et al, 2004), which is considered to be an adaptation that inhibits predation by e.g. rotifers (Luo et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…It is possible that this strain should be classified as another species or within a different genus, especially considering recent taxonomic revisions of Chlorella-like algae (e.g. Krienitz et al, 2004;Luo et al, 2009). In general -and our microcosm studies are no exception -analyses using experimental models focus on the interactions between component species, rather than their phylogenetic status and natural history.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The phylogenetic position and phenotypic changes of a Chlorella-like alga during 5-year microcosm culture

Germond

Hata

Fujikawa

et al. 2013

European Journal of Phycology

View full text Add to dashboard Cite

Recent phylogenetic studies of Chlorella-like algae revealed that this group has diversified into free-living and endosymbiotic niches, the latter within protists and invertebrate organisms. Our previous studies using a long-term culture composed of an alga (Chlorella vulgaris, rDNA sequence unknown), a bacterium (Escherichia coli) and a ciliate (Tetrahymena thermophila), suggested that some clones in the algal population developed an endosymbiotic ability with T. thermophila while others developed an ectosymbiotic ability with E. coli from a non-associated stage. In this paper, an rDNA (18S, ITS1, 5.8S, ITS2)-based phylogeny of the ancestral strain and derived clones isolated from 5-year microcosm cultures was constructed and revealed that the alga belongs to the genus Micractinium. This result was supported by analysis of the secondary structure of the rRNA gene. No difference was observed in the sequence between the ancestral and derived clones. On the other hand, some morphological and physiological traits of the ancestral strain and the derived clones were analysed and revealed that several phenotypic changes had occurred among the algal clones. In particular, the derived clones obtained from long-term cultures had increased in cell size, changed in their ability to grow at pH 4.0, and developed a tendency for cell-aggregation or sedimentation.

show abstract

“…Parachlorella beyerinckii CK-5, an unicellular green algae approximately 3 to 8 μm in diameter, was used in C. vulgaris based on its morphological characteristics according to the description of Fott and Nov'akov'a (1969), and has been P. beyerinckii based on both the sequences of the 18S rRNA gene and its morphological characteristics according to the description of Krienitz et al (2004). The algae cells were cultured in an outdoor pool, harvested, and washed with water by a centrifuge separator.…”

Section: Parachlorella Beyerinckiimentioning

confidence: 99%

The influence of Parachlorella beyerinckii CK-5 on the absorption and excretion of methylmercury (MeHg) in mice

Uchikawa¹,

Yasutake

Kumamoto³

et al. 2010

J. Toxicol. Sci.

View full text Add to dashboard Cite

-Chlorella (Parachlorella beyerinckiiChlorella vulgaris CK-5, is a unicellular green algae that has for many years been used as a nutritional supplement. In order Chlorella, we examined the absorption and excretion of MeHg in mice. Female C57BL/6N mice were randomly divided into three groups 5 mg (4 mg Hg)/kg body weight with or without 100 mg/mouse of P. beyerinckii powder (BP), and were assigned to either a MeHg group or MeHg + BP group, accordingly. Twenty-four hr after oral administration, feces and urine were collected, and blood, liver, and kidney samples were obtained. Total mercury contents in the samples obtained were determined using an atomic absorption method. The amounts of Hg excreted in feces and urine of the MeHg + BP group were increased nearly 1.9 and 2.2-fold compared -ent between two groups. These results suggest that the intake of BP may induce the excretion of Hg both in feces and urine, although it does not affect MeHg absorption from the gastrointestinal tract. The effect of BP on the tissue mercury accumulation may become evident in a long-term experiment.Parachlorella beyerinckii, MeHg, Chlorella

show abstract

Phylogenetic relationship of Chlorella and Parachlorella gen. nov. (Chlorophyta, Trebouxiophyceae)

Cited by 214 publications

References 24 publications

Unique picoeukaryotic algal community under multiple environmental stress conditions in a shallow, alkaline pan

Unique picoeukaryotic algal community under multiple environmental stress conditions in a shallow, alkaline pan

The phylogenetic position and phenotypic changes of a Chlorella-like alga during 5-year microcosm culture

The influence of Parachlorella beyerinckii CK-5 on the absorption and excretion of methylmercury (MeHg) in mice

Contact Info

Product

Resources

About