Phylogenetic reconstructions of the Haptophyta inferred from 18S ribosomal DNA sequences and available morphological data

Edvardsen, Bente; Eikrem, Wenche; Green, J. C.; Andersen, Robert A.; Staay, S.Y. Moon-van der; Medlin, Linda K.

doi:10.2216/i0031-8884-39-1-19.1

Cited by 186 publications

(148 citation statements)

References 84 publications

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“…This is particularly true for taxa for which classification was previously controversial due to the lack of clear morphological criteria. Successful integration of morphological and molecular taxonomy has been demonstrated for many groups of marine flagellates, for example, for cryptomonads (Hoef-Emden & Melkonian, 2003;Cerino & Zingone, 2006, 2007 and prymnesiophytes (Edvardsen et al, 2000;Medlin & Zingone, 2007;Edvardsen et al, 2011). Nevertheless, attempts to achieve such a synthesis with raphidophytes have thus far been more problematic, mainly because of the lack of correspondence between morphological and molecular classifications.…”

Section: Discussionmentioning

confidence: 99%

Phylogeny and morphology of a Chattonella (Raphidophyceae) species from the Mediterranean Sea: what is C. subsalsa?

Klöpper

John

Zingone

et al. 2013

European Journal of Phycology

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We analysed the molecular and morphological features of strains of Chattonella subsalsa isolated from the western Adriatic coast (Mediterranean Sea), with the aim of confirming their classification and elucidating their phylogenetic positions within the Raphidophyceae. We sequenced parts of the ribosomal operon, including the small subunit (SSU), the internal transcribed spacer region (ITS) and the large subunit (LSU) of the rDNA. Additionally, we analysed sequences of the chloroplast-encoded subunit psaA of Photosystem I (PSI) and rbcL, encoding the large subunit of the Rubisco gene. For three phylogenetic markers (LSU, ITS, rbcL), the sequences of the strains from the Adriatic Sea were identical and for two markers (SSU, psaA) only minor differences occurred. All strains were sister to, but well separated from, sequences from isolates in culture collections and from GenBank, thus far classified as belonging to C. subsalsa. Light and electron microscopy provided evidence for morphological differences between a strain of C. subsalsa (CCMP217) from the Gulf of Mexico and the isolates from the Adriatic Sea. Differences concerned the shape and arrangement of chloroplasts and the presence of mucocysts and other surface microstructures, which were only observed in isolates from the Adriatic Sea. This is the first evidence for two different taxa classified as C. subsalsa, which are clearly separated on the basis of several genetic markers and also show morphological differences. As compared with strains assigned to C. subsalsa from the NCMA (formerly CCMP) culture collection, the Adriatic strains more closely match the original species description. This would imply that strain CCMP217 and other genetically similar strains should be described under a new species name. Nevertheless, given the high morphological plasticity of Chattonella species, the definition of the true C. subsalsa must be decided based on detailed morphological and molecular analysis of more strains from other geographical areas.

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Section: Discussionmentioning

confidence: 99%

Phylogeny and morphology of a Chattonella (Raphidophyceae) species from the Mediterranean Sea: what is C. subsalsa?

Klöpper

John

Zingone

et al. 2013

European Journal of Phycology

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“…Sequences of haptophyte taxa basal to Prymnesiales in previous phylogenetic studies (e.g. Edvardsen et al, 2000;Medlin et al, 2008) were selected as outgroups to root the trees. Four datasets were generated: 18S (63 taxa, 1838 characters), 28S (37 taxa, 1086 characters), 16S (22 taxa, 701 characters) and a concatenated dataset of the three genes, including taxa where both 18S and 28S rDNA sequences were available (30 taxa, 3625 characters), with the 16S rDNA for 13 taxa (Table 1).…”

Section: Phylogenetic Analysesmentioning

confidence: 99%

“…In our 18S rDNA tree (Fig. 3), clades are numbered according to our previous work (Edvardsen et al, 2000) and newly recognized clades B1-1 to B1-6. The first divergence within the class Prymnesiophyceae (the ingroup) corresponds to the order Phaeocystales, clade A (see Lange et al, 2002 morphology of these cells is known.…”

Section: Molecular Phylogeneticsmentioning

confidence: 99%

“…Early on, Parke and co-workers recognized the great morphological variation within the genus Chrysochromulina, but were reluctant to erect new genera on characters that could only be seen in the electron microscope (Parke et al, 1955). Suggestions that the genus Chrysochromulina is an artificial grouping have also been based on ultrastructural studies (Birkhead & Pienaar, 1995a), 18S rDNA Edvardsen et al, 2000;Edvardsen & Medlin, 2007) and rbcL DNA sequence analysis (Inouye, 1997;Fujiwara et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Ribosomal DNA phylogenies and a morphological revision provide the basis for a revised taxonomy of the Prymnesiales (Haptophyta)

Edvardsen

Eikrem

Throndsen

et al. 2011

European Journal of Phycology

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Nucleotide sequences of the nuclear-encoded small subunit (18S rDNA) and partial large subunit (28S rDNA) ribosomal DNA were determined in 30 different species of the haptophyte genera Prymnesium, Chrysocampanula, Chrysochromulina, Imantonia and Platychrysis, all belonging to the order Prymnesiales. Phylogenies based on these and other available haptophyte 18S, 28S and plastid 16S rDNA sequences were reconstructed, and compared with available morphological and ultrastructural data. The rDNA phylogenies indicate that the genus Chrysochromulina is paraphyletic and is divided into two major clades. This is supported by ultrastructural and morphological data. There is a major split between Chrysochromulina species with a saddle-shaped cell form (clade B2) and the remaining species in the genus (clade B1). Clade B2 includes the type species C. parva and taxa belonging to this clade thus retain the name Chrysochromulina. The non-saddle-shaped Chrysochromulina species analysed are closely related to Hyalolithus, Prymnesium and Platychrysis species. Imantonia species are sister taxa to these species within clade B1. An amendment to the classification of the order Prymnesiales and the genera Prymnesium, Platychrysis and Chrysochromulina is proposed with one new and one emended family (Chrysochromulinaceae and Prymnesiaceae, respectively), two new genera (Haptolina and Pseudohaptolina), and one new species (Pseudohaptolina arctica). We suggest a revision of the taxonomy of the Prymnesiales that is in accordance with available molecular evidence and supported by morphological data.

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“…However, both these higher group probes signal intensities were positively correlated except for PrymS02_25_dT normalised to DunGS02_25_dT_dT, which produced a negative linear regression (Table III- Table III-S3) for Prymnesiophyceae normalised signal intensity with both control probes had a positive linear relationship R 2 ≥ 0.84; but, only the normalised POSITIVE_25_dT curve was significant (P = 0.0276). Both the clade level probes (Clade01old_25_dT (Prymnesium) and Clade01new25_dT (Prymnesium B1 clade sensu Edvardsen et al, 2000) normalised signal intensities were positively correlated R 2 ≥ 0.73 and significant (P ≤ 0.0477) except for Clade01new25_dT normalised to DunGS02_25_dT_dT (Table III-2). Two species-specific probes for P. parvum were redesigned for the third generation chip from original sequences in the 28S (PparvD01_25_dT; and 18S regions (Prymparv01_25_dT; (Table III-S3).…”

Section: Prymnesium Parvummentioning

confidence: 94%

An evaluation of the applicability of microarrays for monitoring toxic algae in Irish coastal waters

McCoy

Touzet

Fleming

et al. 2012

Environ Sci Pollut Res

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Phylogenetic reconstructions of the Haptophyta inferred from 18S ribosomal DNA sequences and available morphological data

Cited by 186 publications

References 84 publications

Phylogeny and morphology of a Chattonella (Raphidophyceae) species from the Mediterranean Sea: what is C. subsalsa?

Phylogeny and morphology of a Chattonella (Raphidophyceae) species from the Mediterranean Sea: what is C. subsalsa?

Ribosomal DNA phylogenies and a morphological revision provide the basis for a revised taxonomy of the Prymnesiales (Haptophyta)

An evaluation of the applicability of microarrays for monitoring toxic algae in Irish coastal waters

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