Phylogenetic reconstruction within Mycobacterium tuberculosis Beijing genotype in northwestern Russia

Mokrousov, Igor; Narvskaya, Olga; Otten, Tatiana; Vyazovaya, Anna; Limeschenko, Elena; Steklova, Lidia; Vyshnevskyi, Boris

doi:10.1016/s0923-2508(02)01374-8

Cited by 91 publications

(83 citation statements)

References 44 publications

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“…With the increased availability of genotyping data, the Beijing strains were proved more heterogeneous than initially estimated, and several Beijing sublineages have been identified (28)(29)(30)(31). However, a full understanding of the genetic diversity of Beijing family is constrained by the low amount of nucleotide variation (8,32).…”

Section: Significancementioning

confidence: 99%

Southern East Asian origin and coexpansion of Mycobacterium tuberculosis Beijing family with Han Chinese

Luò

Comas

Luo

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

146

144

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The Beijing family is the most successful genotype of Mycobacterium tuberculosis and responsible for more than a quarter of the global tuberculosis epidemic. As the predominant genotype in East Asia, the Beijing family has been emerging in various areas of the world and is often associated with disease outbreaks and antibiotic resistance. Revealing the origin and historical dissemination of this strain family is important for understanding its current global success. Here we characterized the global diversity of this family based on whole-genome sequences of 358 Beijing strains. We show that the Beijing strains endemic in East Asia are genetically diverse, whereas the globally emerging strains mostly belong to a more homogenous subtype known as “modern” Beijing. Phylogeographic and coalescent analyses indicate that the Beijing family most likely emerged around 30,000 y ago in southern East Asia, and accompanied the early colonization by modern humans in this area. By combining the genomic data and genotyping result of 1,793 strains from across China, we found the “modern” Beijing sublineage experienced massive expansions in northern China during the Neolithic era and subsequently spread to other regions following the migration of Han Chinese. Our results support a parallel evolution of the Beijing family and modern humans in East Asia. The dominance of the “modern” Beijing sublineage in East Asia and its recent global emergence are most likely driven by its hypervirulence, which might reflect adaption to increased human population densities linked to the agricultural transition in northern China.

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Section: Significancementioning

confidence: 99%

Southern East Asian origin and coexpansion of Mycobacterium tuberculosis Beijing family with Han Chinese

Luò

Comas

Luo

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

146

144

View full text Add to dashboard Cite

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“…In other areas (e.g., Cuba, South Africa, countries of the former Soviet Union, and Vietnam), Beijing strains are emerging and associated with resistance to anti-TB drugs (1). The Beijing clade comprises at least 2 major subgroups, which share the characteristic spoligotype pattern (6)(7)(8): typical and atypical Beijing strains. Typical ("modern" [8,9]) Beijing strains, including W strains (7), exhibit highly similar, multicopy insertion sequence (IS) 6110 restriction fragment length polymorphism (RFLP) patterns and have alterations in putative mutator genes (4,10).…”

Section: Ycobacterium Tuberculosismentioning

confidence: 99%

“…The Beijing clade comprises at least 2 major subgroups, which share the characteristic spoligotype pattern (6)(7)(8): typical and atypical Beijing strains. Typical ("modern" [8,9]) Beijing strains, including W strains (7), exhibit highly similar, multicopy insertion sequence (IS) 6110 restriction fragment length polymorphism (RFLP) patterns and have alterations in putative mutator genes (4,10). Atypical ("ancestral" [8,9]) Beijing strains more closely resemble the common ancestor of the Beijing clade (6)(7)(8)10).…”

Section: Ycobacterium Tuberculosismentioning

confidence: 99%

“…Typical ("modern" [8,9]) Beijing strains, including W strains (7), exhibit highly similar, multicopy insertion sequence (IS) 6110 restriction fragment length polymorphism (RFLP) patterns and have alterations in putative mutator genes (4,10). Atypical ("ancestral" [8,9]) Beijing strains more closely resemble the common ancestor of the Beijing clade (6)(7)(8)10). The ability of these Beijing clade subgroups to gain resistance or circumvent BCG vaccineinduced immunity may differ and thus explain the differences in geographic distribution of Beijing strains and the variation in association with drug resistance.…”

Section: Ycobacterium Tuberculosismentioning

confidence: 99%

“…The ability of these Beijing clade subgroups to gain resistance or circumvent BCG vaccineinduced immunity may differ and thus explain the differences in geographic distribution of Beijing strains and the variation in association with drug resistance. However, few studies have distinguished between subgroups of the Beijing clade, or studies were limited in the number of strains analyzed (8,9,11,12).…”

Section: Ycobacterium Tuberculosismentioning

confidence: 99%

See 2 more Smart Citations

Vaccine-induced Immunity Circumvented by TypicalMycobacterium tuberculosisBeijing Strains

Kremer¹,

Werf²,

Au³

et al. 2009

Emerg. Infect. Dis.

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The frequency of typical and atypical Beijing strains of Mycobacterium tuberculosis was determined in the Netherlands; Vietnam; and Hong Kong Special Administrative Region, People’s Republic of China. The strains’ associations with drug resistance, M. bovis BCG vaccination, and patient characteristics were assessed. BCG vaccination may have positively selected the prevalent typical Beijing strains.

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PCR-Based Methodology for Detecting Multidrug-Resistant Strains of Mycobacterium tuberculosis Beijing Family Circulating in Russia

Mokrousov

Otten

Vyazovaya

et al. 2003

European Journal of Clinical Microbiology & Infectious Dise

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The Beijing genotype of Mycobacterium tuberculosis has been identified in 40-50% of the clinical isolates studied in Russia during the last decade. This genotype has been reported to be associated with multiple drug resistance and possesses some significant pathogenic properties. Therefore, early identification of such strains is of extreme importance in the timely detection of drug resistance. The present study was performed on 354 strains isolated in Russia from 1996 to 2002 and previously characterised by IS 6110-restriction fragment length polymorphism (RFLP) typing and spoligotyping. These strains included 198 Beijing family strains and 156 strains of other genotypes (IS 6110-RFLP profiles). A subsequent polymerase chain reaction (PCR) analysis with IS 6110-derived outwardly oriented primers (IS 6110-PCR) easily discriminated the Beijing strains from non-Beijing strains. The multiplex allele-specific (MAS)-PCR assays were further used to detect mutations in katG315 and rpoB531, associated with resistance to isoniazid and rifampin, respectively. The katG315 and rpoB531 mutations were found to be more prevalent among Beijing (96.8% and 77.3%) than among non-Beijing strains (85.7% and 28%). Consequently, we propose a two-step methodology based on routine PCR and simple agarose gel electrophoresis in order to detect (i) a Beijing family strain using IS 6110-PCR, and, (ii) its possible resistance to the major anti-tuberculosis drugs using specific MAS-PCR assays.

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Phylogenetic reconstruction within Mycobacterium tuberculosis Beijing genotype in northwestern Russia

Cited by 91 publications

References 44 publications

Southern East Asian origin and coexpansion of Mycobacterium tuberculosis Beijing family with Han Chinese

Southern East Asian origin and coexpansion of Mycobacterium tuberculosis Beijing family with Han Chinese

Vaccine-induced Immunity Circumvented by TypicalMycobacterium tuberculosisBeijing Strains

PCR-Based Methodology for Detecting Multidrug-Resistant Strains of Mycobacterium tuberculosis Beijing Family Circulating in Russia

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