Phylogenetic Positioning of Acetobacter, Gluconobacter, Rhodopila and Acidiphilium Species as a Branch of Acidophilic Bacteria in the α-subclass of Proteobacteria Based on 16S Ribosomal DNA Sequences

Sievers, Martin; Ludwig, Wolfgang; Teuber, Michael

doi:10.1016/s0723-2020(11)80006-8

Cited by 78 publications

(31 citation statements)

References 18 publications

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“…With these techniques, Entani et al (1985) characterized an organism involved in submerged vinegar fermentations and later on Sievers et al (1992) described a species, A . europaeus, present in vinegar fermenters in Central Europe.…”

Section: Discussionmentioning

confidence: 99%

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Description of Acetobacter oboediens sp. nov. and Acetobacter pomorum sp. nov., two new species isolated from industrial vinegar fermentations

Sokollek

Hertel

Hammes

1998

International Journal of Systematic Bacteriology

129

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S t uttg a rt, GermanyTwo strains of Acetobacter sp., LTH 2460T and LTH 2458T, have been isolated from running red wine and cider vinegar fermentations, respectively. Taxonomic characteristics of the isolates were investigated. Comparative analysis of the 165 rRNA sequences revealed > 99% similarity between strain LTH 2460T and the type strains of the related species Acetobacter europaeus and Acetobacter xylinus and between strain LTH 2458T and Acetobacter pasteurianus. On the other hand, low levels of DNA relatedness (< 34%) were determined in DNA-DNA similarity studies. This relatedness below the species level was consistent with specific physiological characteristics permitting clear identification of these strains within established species of acetic acid bacteria. Based on these results, the names Acetobacter oboediens sp. nov. and Acetobacterpomorum sp. nov. are proposed for strains LTH 2460T and LTH 2458T, respectively. The phylogenetic positions of the new species are reflected by a 165 rRNA-based tree. Furthermore, a 165 rRNA-targeted oligonucleotide probe specific for A. oboediens was constructed.

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Section: Discussionmentioning

confidence: 99%

“…A . europaeus is also present in high acetic acid vinegar fermentations and was shown to be dependent on the presence of acetic acid in the growth medium (Sievers et al, 1992). The related species A .…”

Section: Discussionmentioning

confidence: 99%

Description of Acetobacter oboediens sp. nov. and Acetobacter pomorum sp. nov., two new species isolated from industrial vinegar fermentations

Sokollek

Hertel

Hammes

1998

International Journal of Systematic Bacteriology

129

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“…Thus either the SS-rRNA and RecA genes of A. facilis have different phylogenetic histories, or one of the trees is inaccurate. The grouping of Acidiphilium species within the α subgroup appears to be a reliable representation of the SSrRNA relationships Sievers et al 1994), so it is unlikely that the SS-rRNA tree here is biased by species sampling. It has been suggested that the A. facilis RecA sequence contains many sequencing errors and it is currently being resequenced (Roca 1995).…”

Section: Proteobacteriamentioning

confidence: 97%

The RecA protein as a model molecule for molecular systematic studies of bacteria: Comparison of trees of RecAs and 16S rRNAs from the same species

1995

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The evolution of the RecA protein was analyzed using molecular phylogenetic techniques. Phylogenetic trees of all currently available complete RecA proteins were inferred using multiple maximum parsimony and distance matrix methods. Comparison and analysis of the trees reveal that the inferred relationships among these proteins are highly robust. The RecA trees show consistent subdivisions corresponding to many of the major bacterial groups found in trees of other molecules including the α, β, γ, δ, and ε Proteobacteria, cyanobacteria, high-GC grampositives, and the Deinococcus-Thermus group. However, there are interesting differences between the RecA trees and these other trees. For example, in all the RecA trees the proteins from gram-positives species are not monophyletic. In addition, the RecAs of the cyanobacteria consistently group with the RecAs of the high-GC gram-positives. To evaluate possible causes and implications of these and other differences, phylogenetic trees were generated for small-subunit rRNA sequences from the same (or closely related) species as represented in the RecA analysis. The trees of the two molecules using these equivalent species-sets are highly congruent and have similar resolving power for close, medium, and deep branches in the history of bacteria. The implications of the particular similarities and differences between the trees are discussed. Some of the features that make RecA useful for molecular systematics and for studies of protein evolution are also discussed.

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“…Genotypic relationships among acetic acid bacteria have been studied by DNA-DNA hybridization (Entani et al, 1985 ;Mason & Claus, 1989 ;Micales et al, 1985 ;Sievers et al, 1992 ;Urakami et al, 1989 ;Yamada et al, 1984), DNA-RNA hybridization (Gillis & De Ley, 1980) and 5S and 16S rDNA sequence analysis (Bulygina et al, 1992 ;Sievers et al, 1994 ;Yamada et al, 1997). The most recent approaches to classification within the family Acetobacteraceae (Gillis et al, 1989 ;Mason & Claus, 1989 ;Urakami et al, 1989 ;Yamada & Kondo, 1984 ;Yamada et al, 1997) reveal the existence of four well- (Sokollek et al, 1998 ;Boesch et al, 1998 ;Franke et al, 1999).…”

Section: Abbreviation : Its Internal Transcribed Spacermentioning

confidence: 99%

“…They are classified in the family Acetobacteraceae as a branch of the acidophilic bacteria in the α-subdivision of the Proteobacteria (De Ley et al, 1984 ;Sievers et al, 1994). Members of the family are recognized by their unique ability to oxidize ethanol to acetic acid in neutral and acidic (pH 4n5) media.…”

Section: Introductionmentioning

confidence: 99%

Identification of acetic acid bacteria by RFLP of PCR-amplified 16S rDNA and 16S-23S rDNA intergenic spacer.

Ruiz¹,

Poblet²,

Mas³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

122

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DNA corresponding to 16S rDNA and the 16S-23S rDNA intergenic spacer (ITS) from 22 reference strains of acetic acid bacteria, representing the diversity of the family Acetobacteraceae, and 24 indigenous acetic acid bacteria isolated from wine fermentations were analysed by PCR-RFLP. Frateuria aurantia LMG 1558 T and Escherichia coli ATCC 11775 T were included as outgroups. PCRamplified products of about 1450 bp were obtained from the 16S rDNA of all the strains and products of between 675 and 800 bp were obtained from the 16S-23S rDNA ITS. PCR products were digested with 4-base-cutting restriction enzymes in order to evaluate the degree of polymorphism existing among these strains. Of the enzymes tested, TaqI and RsaI were the most discriminatory and showed no intraspecific variations in the restriction patterns. Restriction analysis of the 16S rDNA with these enzymes is proposed as a rapid and reliable method to identify acetic acid bacteria at the level of genus and species (or related species group) and its applicability to identification of indigenous acetic acid bacteria was demonstrated. The same degree of distinction as that for the 16S rDNA analysis was obtained within reference strains of acetic acid bacteria by PCR-RFLP of the 16S-23S rDNA ITS. However, 16S-23S rDNA ITS restriction patterns of strains isolated from wine did not match those of any of the reference strains. Thus, PCR-RFLP of the 16S-23S rDNA ITS is not a useful method to identify isolates of acetic acid bacteria at the species level, although it may be an adequate method to detect intraspecific differentiation.

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Phylogenetic Positioning of Acetobacter, Gluconobacter, Rhodopila and Acidiphilium Species as a Branch of Acidophilic Bacteria in the α-subclass of Proteobacteria Based on 16S Ribosomal DNA Sequences

Cited by 78 publications

References 18 publications

Description of Acetobacter oboediens sp. nov. and Acetobacter pomorum sp. nov., two new species isolated from industrial vinegar fermentations

Description of Acetobacter oboediens sp. nov. and Acetobacter pomorum sp. nov., two new species isolated from industrial vinegar fermentations

The RecA protein as a model molecule for molecular systematic studies of bacteria: Comparison of trees of RecAs and 16S rRNAs from the same species

Identification of acetic acid bacteria by RFLP of PCR-amplified 16S rDNA and 16S-23S rDNA intergenic spacer.

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