Phylogenetic Position of Endosymbiotic Green Algae in <i>Paramecium bursaria</i> Ehrenberg from Japan

Hoshina, Ryo; Kamako, Shin‐ichiro; Imamura, Nobutaka

doi:10.1055/s-2004-820888

Cited by 46 publications

(36 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Smith) Hinda´k and D. palatina Schmidle (formerly Scenedesmaceae) and Micractinium pusillum Fresenius (formerly Micractiniaceae). On the other hand, Hoshina et al (2004) have discussed the phylogenetic position of endosymbiotic green algae in Paramecium bursaria Ehrenberg. These algae would be genetically closer to the C. vulgaris group than to the (at that time) P. kessleri group.…”

Section: Discussionmentioning

confidence: 99%

Late type of daughter cell wall synthesis in one of the Chlorellaceae, Parachlorella kessleri (Chlorophyta, Trebouxiophyceae)

Yamamoto

Kurihara

Kawano

2005

Planta

View full text Add to dashboard Cite

Autosporulation is a common mode of propagation for unicellular algae. Autospore-forming species of Chlorellaceae, Chlorella vulgaris Beijerinck, C. sorokiniana Shihira et Krauss, C. lobophora Andreyeva, and Parachlorella kessleri (Fott et Nováková) Krienitz et al. have glucosamine as the main constituent of their rigid cell wall. Recent phylogenetic analyses have showed that the Chlorellaceae divided into two sister groups: the Chlorella-clade and the Parachlorella-clade. We compared the cell wall structure and synthesis of the daughter cell wall in the four species by electron microscopy using rapid freezing and freeze substitution methods. The cell wall of C. vulgaris, C. sorokiniana, and C. lobophora consisted of an electron-dense thin layer with an average thickness of 17-20, 22, and 19 nm, respectively. In these three species, daughter cell wall synthesis occurred on the outer surface of the plasma membrane in the early cell-growth phase. The cell wall of P. kessleri, however, was electron-transparent and 54-59 nm in thickness. Ruthenium red staining of P. kessleri indicated that ruthenium-red-specific polysaccharides accumulated over the outer surface of the plasma membrane. Immunoelectron microscopic observation with an anti-beta-1, 3-glucan antibody and staining with wheat germ agglutinin (WGA) indicated that the cell wall contained beta-1, 3-glucan and WGA specific N-acetyl-beta-D-glucosamine. In P. kessleri, daughter cell wall synthesis began after successive protoplast division. The daughter cell wall synthesis during autosporulation in the four species of Chlorellaceae can be classified into two types-the early and the late types.

show abstract

Section: Discussionmentioning

confidence: 99%

Late type of daughter cell wall synthesis in one of the Chlorellaceae, Parachlorella kessleri (Chlorophyta, Trebouxiophyceae)

Yamamoto

Kurihara

Kawano

2005

Planta

View full text Add to dashboard Cite

show abstract

“…Polymerase chain reaction (PCR) was carried out to amplify SSU to ITS rDNA by using the primer pairs SR-1/SR-9 [22], SR-6 [22]/SR-12 k [23], INT-4 F [24]/ITS4 [25] and INT-4 F/HLR-3R [23]. PCR products were purified with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany), and were directly sequenced by using the Operon DNA sequencing service (Operon Biotechnology, Tokyo).…”

Section: Methodsmentioning

confidence: 99%

DNA analyses of a private collection of microbial green algae contribute to a better understanding of microbial diversity

Hoshina

2014

BMC Res Notes

View full text Add to dashboard Cite

BackgroundDNA comparison is becoming the leading approach to the analysis of microbial diversity. For eukaryotes, the internal transcribed spacer 2 (ITS2) has emerged as a conspicuous molecule that is useful for distinguishing between species. Because of the small number of usable ITS data in GenBank, ITS2 sequence comparisons have only been used for limited taxa. However, major institutions with planktonic algal culture collections have now released small subunit (SSU) to ITS rDNA sequence data for their collections. This development has uplifted the level of molecular systematics for these algae.ResultsForty-three strains of green algae isolated from German inland waters were investigated by using SSU-ITS rDNA sequencing. The strains were isolated through the direct plating method. Many of the strains went extinct during the years of culture. Thus, it could be expected that the surviving strains would be common, vigorous species. Nevertheless, 12 strains did not match any known species for which rDNA sequences had been determined. Furthermore, the identity of one strain was uncertain even at the genus level.ConclusionsThe aforementioned results show that long-forgotten and neglected collections may be of great significance in understanding microbial diversity, and that much work still needs to be done before the diversity of freshwater green algae can be fully described.

show abstract

“…Whole P. bursaria DNA extracts were used to amplify both host SSU rDNA and photobiont SSU rDNA. Host targeting PCR was performed with the primer pair SR-1 (universal [27])/Paramecium800R (host specific [6]), and algae targeting PCR was also performed with CHspeRmaeF (trebouxiophyte specific [6])/INT-5R (trebouxio- phyte specific [5]) for the photobiont. The PCR product of the host DNA was purified via Quantum Prep PCR Kleen Spin Columns (Bio-Rad, CA) and directly sequenced.…”

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

Photobiont Flexibility in <i>Paramecium bursaria</i>: Double and Triple Photobiont Co-Habitation

Hoshina¹,

Fujiwara²

2012

AiM

View full text Add to dashboard Cite

The green ciliate, Paramecium bursaria, has evolved a mutualistic relationship with endosymbiotic green algae (photobionts). Under culture conditions, photobionts are usually unified (to be single species) within each P. bursaria strain. In most cases, the algal partners are restricted to either Chlorella variabilis or Micractinium reisseri (Chlorellaceae, Trebouxiophyceae). Both species are characterized by particular physiology and atypical group I intron insertions, although they are morphologically indistinguishable from each other or from other Chlorella-related species. Both algae are exclusive species that are viable only within P. bursaria cells, and therefore their symbiotic relationship can be considered persistent. In a few cases, the other algal species have been reported as P. bursaria photobionts. Namely, P. bursaria have occasionally replaced their photobiont partner. This paper introduces some P. bursaria strains that maintain more than one species of algae for a long period. This situation prompts speculations about flexibility of host-photobiont relationships, how P. bursaria replaced these photobionts, and the infection theory of the group I introns.

show abstract

Phylogenetic Position of Endosymbiotic Green Algae in Paramecium bursaria Ehrenberg from Japan

Cited by 46 publications

References 39 publications

Late type of daughter cell wall synthesis in one of the Chlorellaceae, Parachlorella kessleri (Chlorophyta, Trebouxiophyceae)

Late type of daughter cell wall synthesis in one of the Chlorellaceae, Parachlorella kessleri (Chlorophyta, Trebouxiophyceae)

DNA analyses of a private collection of microbial green algae contribute to a better understanding of microbial diversity

Photobiont Flexibility in <i>Paramecium bursaria</i>: Double and Triple Photobiont Co-Habitation

Contact Info

Product

Resources

About

Phylogenetic Position of Endosymbiotic Green Algae in Paramecium bursaria Ehrenberg from Japan

Cited by 46 publications

References 39 publications

Late type of daughter cell wall synthesis in one of the Chlorellaceae, Parachlorella kessleri (Chlorophyta, Trebouxiophyceae)

Late type of daughter cell wall synthesis in one of the Chlorellaceae, Parachlorella kessleri (Chlorophyta, Trebouxiophyceae)

DNA analyses of a private collection of microbial green algae contribute to a better understanding of microbial diversity

Photobiont Flexibility in &lt;i&gt;Paramecium bursaria&lt;/i&gt;: Double and Triple Photobiont Co-Habitation

Contact Info

Product

Resources

About

Photobiont Flexibility in <i>Paramecium bursaria</i>: Double and Triple Photobiont Co-Habitation