Phylogenetic position of Dunaliella acidophila (Chlorophyceae) based on ITS and rbcL sequences

Assunçao, Patricia A. Clemente Janeiro De; Jaén‐Molina, Ruth; Caujapé‐Castells, Juli; Jara, Adelina de la; Carmona, Lourdes; Freijanes, Karen; Mendoza, H.

doi:10.1007/s10811-011-9676-1

Cited by 17 publications

(14 citation statements)

References 35 publications

(31 reference statements)

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“…Nevertheless, the other freshwater species analyzed in this study ( D. acidophila , CCAP19/35), maintained its position within the subgenus Dunaliella , and was not phylogenetically related to D. lateralis , as recently proposed [28]. Furthermore, the observation that different species belonging to several Sections ( Tertiolecta, Viridis, Dunaliella and Peircei ) share the exact same ITS2 sequence, make us believe that they correspond to a single species.…”

Section: Discusionsupporting

confidence: 62%

Molecular taxonomy of Dunaliella (Chlorophyceae), with a special focus on D. salina: ITS2 sequences revisited with an extensive geographical sampling

et al. 2012

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We used an ITS2 primary and secondary structure and Compensatory Base Changes (CBCs) analyses on new French and Spanish Dunallela salina strains to investigate their phylogenetic position and taxonomic status within the genus Dunaliella. Our analyses show a great diversity within D. salina (with only some clades not statistically supported) and reveal considerable genetic diversity and structure within Dunaliella, although the CBC analysis did not bolster the existence of different biological groups within this taxon. The ITS2 sequences of the new Spanish and French D. salina strains were very similar except for two of them: ITC5105 "Janubio" from Spain and ITC5119 from France. Although the Spanish one had a unique ITS2 sequence profile and the phylogenetic tree indicates that this strain can represent a new species, this hypothesis was not confirmed by CBCs, and clarification of its taxonomic status requires further investigation with new data. Overall, the use of CBCs to define species boundaries within Dunaliella was not conclusive in some cases, and the ITS2 region does not contain a geographical signal overall.

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Section: Discusionsupporting

confidence: 62%

Molecular taxonomy of Dunaliella (Chlorophyceae), with a special focus on D. salina: ITS2 sequences revisited with an extensive geographical sampling

et al. 2012

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“…In previous publications we detected clear taxonomic discrepancies between the traditional, morphology‐based taxonomy and phylogenetic analyses, suggesting that the taxonomy of the whole genus Dunaliella should be re‐examined (Assunção et al . , ). Some of the aspects highlighted in those publications were (i) that different species belonging to several Dunaliella sections could correspond to a single species (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…, , ; Assunção et al . , ), (ii) an automatic approach to its analysis has become available, additionally providing a tool for species delimitation (Schultz & Wolf ; Koetschan et al . ), and (iii) it is one of the regions considered to be used for DNA barcoding in green algae (Buchheim et al .…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of ITS2 sequences suggests the taxonomic re‐structuring of Dunaliella viridis (Chlorophyceae, Dunaliellales)

Assunçao¹,

Jaén‐Molina

Caujapé‐Castells

et al. 2013

Phycological Research

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Summary We analyzed the ITS2 primary and secondary structure (including Compensatory Base Changes (CBCs)) of 17 new Dunaliella strains (11 D. viridis, two D. tertiolecta, and four Dunaliella sp.), and compared these with other Dunaliella sequences available from the ITS2 database to circumscribe their taxonomic position. The ITS2 primary and secondary structure analysis positioned the majority of D. viridis strains in four main clades, showing that D. viridis is polyphyletic. The detection of at least one CBC among these clades strongly suggests that they could correspond to different biological species. Unexpectedly, while D. viridis var. euchlora (CCAP19/21) was positioned within the subgenus Dunaliella, D. viridis var. palmelloides (CCAP11/34) was positioned clearly outside this subgenus, suggesting that this taxon may not be properly placed in Dunaliella. Furthermore, the detection of at least three compensatory base changes (CBCs) between D. viridis var. palmelloides (CCAP11/34) and the other strains analyzed, confirm that this strain is a different species. For these reasons we propose re‐naming D. viridis var. palmelloides (CCAP11/34) to incertae sedis, and D. viridis var. euchlora (CCAP19/21) to Dunaliella sp. Therefore, the ITS2 primary and secondary structure data suggest a taxonomic re‐structuring of D. viridis.

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“…MBTD-CMFRI-S086 (JN797810), which is sister to the above clade with ML bootstrap values and BI posterior probabilities of 45.6% and 0.91, respectively. The ML tree was rooted using a published rbcL sequence of Chlamydomonas reinhardtii P. A. Dangeard as an outgroup [29]. In the BI tree obtained using aligned psaB gene sequences (Fig.…”

Section: Phylogenetic Analysesmentioning

confidence: 99%

Phylogenetic and Morphological Investigation of a Dunaliella Strain Isolated from Yuncheng Salt Lake, China

Wang¹,

Feng

Xie³

2014

PLANT

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A Dunaliella strain was isolated from Yuncheng Salt Lake, Shanxi, China (111.05°E, 35.03°N). Morphological characteristics and molecular data were used to evaluate the relationship of this algal strain to other Dunaliella strains. Morphology of the isolated strain observed was close to Dunaliella salina. Phylogenetic trees were constructed from rbcL, psaB, ITS (ITS-1+5.8S rDNA+ITS-2) and 18S rDNA sequence data. Phylogenetic analysis of the four gene sequences revealed that the isolated Dunaliella strain is closely related to D. salina (Dunal) Teodoresco, D. peircei Nicolai et Baas-Becking, D. tertiolecta Butcher and more likely closer to D. salina. Combined morphological characteristics and phylogenetic analysis, the isolated Duanliella-designated here as D. sp YC01-should be a Dunalialla salina strain.

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Phylogenetic position of Dunaliella acidophila (Chlorophyceae) based on ITS and rbcL sequences

Cited by 17 publications

References 35 publications

Molecular taxonomy of Dunaliella (Chlorophyceae), with a special focus on D. salina: ITS2 sequences revisited with an extensive geographical sampling

Molecular taxonomy of Dunaliella (Chlorophyceae), with a special focus on D. salina: ITS2 sequences revisited with an extensive geographical sampling

Phylogenetic analysis of ITS2 sequences suggests the taxonomic re‐structuring of Dunaliella viridis (Chlorophyceae, Dunaliellales)

Phylogenetic and Morphological Investigation of a Dunaliella Strain Isolated from Yuncheng Salt Lake, China

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