Phylogenetic origin of <i>Magnolia pseudokobus</i> (Magnoliaceae), a rare <i>Magnolia</i> extinct in the wild, revealed by chloroplast genome sequencing, genome-wide SNP genotyping and microsatellite analysis

Sakaguchi, Shota; Nagasawa, Koki; Umetsu, Yukie; Nagasawa, Jun ichi; Ichikawa, S.; Kinoshita, Satoru; Hiratsuka, Ken ichi; Suyama, Yoshihisa; Tsunamoto, Yoshihiro; Isagi, Yuji; Setoguchi, Hiroaki

doi:10.1080/13416979.2020.1767268

Cited by 2 publications

(1 citation statement)

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“…The method starts with multiplex polymerase chain reaction (PCR) using inter-simple sequence repeat (ISSR) primers; therefore, it is called multiplexed ISSR genotyping by sequencing (MIG-seq). Although they demonstrated its applicability for individual (clone) identification and population genetics analysis in their original study, recent studies have shown the efficacy of this method in molecular phylogenetic taxonomy in plants and animals Nguyen et al, 2020), including its application to museum specimens (Eguchi et al, 2020;Strijk et al, 2020) and for species identification and discovery in plants (Binh, Ngoc, Bon, et al, 2018;Okabe et al, 2021;Sakaguchi et al, 2020;Zhang et al, 2020) and corals (Richards et al, 2018;Takata et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Complementary combination of multiplex high‐throughput DNA sequencing for molecular phylogeny

Suyama

Hirota

Matsuo

et al. 2021

Ecological Research

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The rapid development of DNA sequencing technology in recent years has provided new tools for phylogenetic data acquisition. By using high-throughput DNA sequencing technology, molecular phylogenetic information can be obtained more quickly and economically. Here, we describe a complementary combination of two multiplex high-throughput DNA sequencing methods. One is multiplexed phylogenetic marker sequencing (MPM-seq), and the other is multiplexed inter-simple sequence repeat (ISSR) genotyping by sequencing (MIGseq), whose protocol is improved over that of the original one. Both MPM-seq and MIG-seq begin with multiplex polymerase chain reaction (PCR), each amplifying multiple phylogenetic markers and genome-wide ISSR regions, respectively.After another PCR using a second PCR primer set that is common in both methods, next-generation sequencing is used to simultaneously detect DNA sequences of multiple regions from multiple samples in each method. In this case study, we performed a molecular phylogenetic analysis of Japanese fir (Abies) and the closely related Abies species. MPM-seq revealed DNA sequences of three regions from chloroplast DNA and one nuclear internal transcribed spacer and created a partially informative phylogenetic tree for 13 Abies species. Whereas MIG-seq detected 6700 single-nucleotide polymorphisms and exhibited clear clustering of related species with 97%-100% bootstrap support for all branches of the phylogenetic tree. Hence, with a complementary combination, quick, simple, and economical analysis can be performed in a wide range of genomic studies, including molecular phylogeny, as well as for investigating genetic differentiation or genetic identification among species, hybrids, and populations, and even among clones and cultivars, as a DNA barcoding technique.

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