Phylogenetic in situ/ex situ analysis of a sulfur mat microbial community from a thermal sulfide spring in the north Caucasus

Chernousova, E. Yu.; Акимов, В. Н.; Gridneva, E. V.; Дубинина, Г. А.; Grabovich, M. Yu.

doi:10.1134/s002626170802015x

Cited by 14 publications

(12 citation statements)

References 12 publications

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“…After centrifugation at 12,000 g for 2 min, the supernatant was used as the DNA template. The PCR reaction was performed using the eubacterial primers (27f and 1492r) as previously described (Chernousova et al, 2008). The amplified partial 16S rDNA fragment was electrophoretically separated in 0.8% agarose gels.…”

Section: Isolation Of Rhizobacteria From Plants Various Plantsmentioning

confidence: 99%

Growth Promotion of Pepper Plants by Pantoea ananatis B1-9 and its Efficient Endophytic Colonization Capacity in Plant Tissues

Kim¹,

Cho²,

Kim³

et al. 2012

The Plant Pathology Journal

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The bacteria B1-9 that was isolated from the rhizosphere of the green onion could promote growth of pepper, cucumber, tomato, and melon plants. In particular, pepper yield after B1-9 treatment on the seedling was increased about 3 times higher than that of control plants in a field experiment. Partial 16S rDNA sequences revealed that B1-9 belongs to the genus Pantoea ananatis. Pathogenecity tests showed non-pathogenic on kimchi cabbage, carrot, and onion. The functional characterization study demonstrated B1-9's ability to function in phosphate solubilization, sulfur oxidation, nitrogen fixation, and indole-3-acetic acid production. To trace colonization patterns of B1-9 in pepper plant tissues, we used DRAQ5™ fluorescent dye, which stains the DNAs of bacteria and plant cells. A large number of B1-9 cells were found on the surfaces of roots and stems as well as in guard cells. Furthermore, several colonized B1-9 cells resided in inner cortical plant cells. Treatment of rhizosphere regions with strain B1-9 can result in efficient colonization of plants and promote plant growth from the seedling to mature plant stage. In summary, strain B1-9 can be successfully applied in the pepper plantation because of its high colonization capacity in plant tissues, as well as properties that promote efficient plant growth.

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Section: Isolation Of Rhizobacteria From Plants Various Plantsmentioning

confidence: 99%

Growth Promotion of Pepper Plants by Pantoea ananatis B1-9 and its Efficient Endophytic Colonization Capacity in Plant Tissues

Kim¹,

Cho²,

Kim³

et al. 2012

The Plant Pathology Journal

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“…Similar to variation in H 2 S concentrations, springs substantially vary in temperature, with reported values ranging from around 5 °C to over 100 °C [35,40,41,47,48,57,76,81]. Temperature variation appears to be related in part to the geographic location of springs as well as the ultimate sources of H 2 S production.…”

Section: Freshwater Sulphide Springs: Occurrence and Environmental Vamentioning

confidence: 86%

“…Conversely, peak concentrations of H 2 S have been reported to exceed 7000 μM in some springs [79]. Currently available data suggest that H 2 S concentrations in most springs lie in between these extremes, with a large number of springs having concentrations towards the lower end of the spectrum (i.e., <2500 μM H 2 S [40,41,44,47,76,78,80]). …”

Section: Freshwater Sulphide Springs: Occurrence and Environmental Vamentioning

confidence: 99%

“…During this process, groundwater is being enriched in H 2 S and ultimately discharged through surface springs [40,43]. Table 1 and [35,40,41,[44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59] [76], but even such low concentrations are biologically relevant and considered toxic for most metazoans [16]. Conversely, peak concentrations of H 2 S have been reported to exceed 7000 μM in some springs [79].…”

Section: Freshwater Sulphide Springs: Occurrence and Environmental Vamentioning

confidence: 99%

“…During the interaction of the water with hot basaltic rock, a diversity of chemicals leach into solution, including sulphate and other sulphur species that are readily transformed into sulphide under the highly reductive conditions [36][37][38]. Second, sulphide springs can be associated with underground oil deposits, where mineral-rich groundwater containing sulphate mixes with hydrocarbons derived from fossil organic matter in the absence of oxygen [39][40][41]. As in marine cold seeps, sulphur-reducing bacteria then reduce sulphate to H 2 S, while oxidizing organic compounds, during energy metabolism [22,42].…”

Section: Freshwater Sulphide Springs: Occurrence and Environmental Vamentioning

confidence: 99%

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Patterns of Macroinvertebrate and Fish Diversity in Freshwater Sulphide Springs

Greenway

Arias‐Rodríguez²,

Diaz³

et al. 2014

Diversity

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Extreme environments are characterised by the presence of physicochemical stressors and provide unique study systems to address problems in evolutionary ecology research. Sulphide springs provide an example of extreme freshwater environments; because hydrogen sulphide's adverse physiological effects induce mortality in metazoans even at micromolar concentrations. Sulphide springs occur worldwide, but while microbial communities in sulphide springs have received broad attention, little is known about macroinvertebrates and fish inhabiting these toxic environments. We reviewed qualitative occurrence records of sulphide spring faunas on a global scale and present a quantitative case study comparing diversity patterns in sulphidic and adjacent non-sulphidic habitats across replicated river drainages in Southern Mexico. While detailed studies in most regions of the world remain scarce, available data suggests that sulphide spring faunas are characterised by low species richness. Dipterans (among macroinvertebrates) and cyprinodontiforms (among fishes) appear to dominate the communities in these habitats. At least in fish, there is evidence for the presence of highly endemic species and populations exclusively inhabiting sulphide springs. We provide a detailed discussion of traits that might predispose certain taxonomic groups to colonize sulphide springs, how colonizers subsequently adapt to cope with sulphide toxicity, and how adaptation may be linked to speciation processes. OPEN ACCESSDiversity 2014, 6 598

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Genomic and morphological characterization of a new Thiothrix species from a sulfide hot spring of the Zmeinaya bay (Northern Baikal, Russia)

Chernitsyna,

Elovskaya,

Bukin

et al. 2024

Antonie van Leeuwenhoek

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Phylogenetic in situ/ex situ analysis of a sulfur mat microbial community from a thermal sulfide spring in the north Caucasus

Cited by 14 publications

References 12 publications

Growth Promotion of Pepper Plants by Pantoea ananatis B1-9 and its Efficient Endophytic Colonization Capacity in Plant Tissues

Growth Promotion of Pepper Plants by Pantoea ananatis B1-9 and its Efficient Endophytic Colonization Capacity in Plant Tissues

Patterns of Macroinvertebrate and Fish Diversity in Freshwater Sulphide Springs

Genomic and morphological characterization of a new Thiothrix species from a sulfide hot spring of the Zmeinaya bay (Northern Baikal, Russia)

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