Phylogenetic groups, virulence genes and quinolone resistance of integron-bearing Escherichia coli strains isolated from a wastewater treatment plant

Mokracka, Joanna; Koczura, Ryszard; Jabłońska, Lucyna; Kaznowski, Adam

doi:10.1007/s10482-011-9555-4

Cited by 48 publications

(39 citation statements)

References 34 publications

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“…Farshad et al (35) reported that the prevalence of all virulent genes was lower in resistant groups of UPEC strains but not statistically significant except for pap and cnf1 with nalidixic acid (35) were similar to our findings, which showed that susceptible UPEC strains had the highest distribution of papG, usp and fim virulence factors. Both quinolone and fluroquinolone resistant strains of previous study harbored lower distribution of pap, hly, cnf1 and sfa virulence genes (37).…”

Section: Discussionmentioning

confidence: 63%

Molecular Characterization and Antimicrobial Resistance of Uropathogenic Escherichia coli

et al. 2014

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Background: Urinary Tract Infections (UTIs) are the most common infectious diseases in childhood. The Uropathogenic Escherichia coli (UPEC) strains account for as much as 80% of UTIs. Objective: From a clinical perspective, it is important to know which virulence factors and antibiotic resistance properties are present in UPEC strains in pediatrics. Therefore, this study was carried out to investigate the molecular characterization and antimicrobial resistance of UPEC strains isolated from hospitalized patients in pediatric ward of Baqiyatallah Hospital in Tehran. Patients and Methods: One hundred and twenty-one urine specimens were collected from the patients infected with UTIs (51 boys and 70 girls). The urine samples were cultured immediately, and those with E. coli-positive were analyzed for the presence of antibiotic resistance genes and bacterial virulence factors using Polymerase Chain Reaction (PCR). Also, antimicrobial susceptibility testing was performed using disk diffusion methodology with Mueller-Hinton agar according to the instruction of Clinical Laboratory and Standard Institute. Results: Nineteen out of 51 (37.25%) urine samples from boys and 47 out of 70 (67.14%) urine samples from girls harbored E. coli. A significant difference was found between the frequency of UPEC strains in boys and girls (P <.05). High resistance levels to tetracycline (69.6%), ampicillin (69.6%) and norfloxacin (63.6%) were also observed. Totally, 1.66% of tested strains were resistant to more than 8 antibiotics. The incidence of genes encoding resistance against gentamicin (aac (3)-IV), sulfonamide (sul1), beta-lactams (blaSHV and CITM), tetracycline (tetA and tetB), trimethoprim (dfrA1), and quinolones (qnr) were 25. 7%, 22.7%, 83.2%, 71.1%, 19.6% and 21.2%, respectively. The most commonly detected virulence factors were fim (71.2%), set-1 (66.6%), iha (62.1), papGI (59%), usp (56%) and sen (22.7%). Conclusion: Resistant strains of uropathogenic E. coli had the lower incidence of uropathogenic virulence factors. We suggested prescription of imipenem and amikacin to treat pediatric patients infected with UTIs.

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Section: Discussionmentioning

confidence: 63%

Molecular Characterization and Antimicrobial Resistance of Uropathogenic Escherichia coli

et al. 2014

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“…[7][8][9] The increase of antibiotic resistance following the use of antibiotics such as beta-lactams and growing the commensal and pathogenic ESBL (extended-spectrum beta-lactamase)-producing bacteria like E. coli strains especially in foodproducing animals pose a threat to the public health and food safety. [10][11][12][13][14] Researchers have suggested that the study of antibiotic resistance in commensal microorganisms like E. coli from the healthy animals would be quite useful, because they can be used as gnomon organisms to measure the prevalence of antibiotic resistance. These organisms could serve as a reservoir for the genes encoding antimicrobial resistance, which is transferred to the pathogenic bacteria or transmitted to the human by direct exposure to animals or via the food chain.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Grouping and Phenotypic Detection of Extended-Spectrum β-Lactamases Among Escherichia coli From Calves and Dairy Cows in Khuzestan, Iran

Barzan¹,

Gharibi²,

Ghorbanpoor³

et al. 2017

Int J Enteric Pathog

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Background: Food-producing animals are under suspicion for the reservoir and colonization of ESBL (extended-spectrum beta-lactamase)-producing bacteria especially Enterobacteriaceae and therefore infection of the humans with them. The increasing reports on the ESBLs presence in the pathogenic and commensal Escherichia coli isolates have been a concern worldwide. These strains can be attributed to one of the main phylogenetic groups and subgroups. Several studies have shown the relationship between the phylogeny and antimicrobial resistance of E. coli strains. Objectives: The aim of this study was to analyze the phylogenetic group of ESBL-producing E. coli and detect its phenotype using the multiplex polymerase chain reaction (PCR) and combined disk method. Materials and Methods: Two hundred five E. coli fecal isolates were obtained from 103 calves (90 healthy and 13 diarrheic) and 102 dairy cows (healthy) from 8 farms in Khuzestan province, Iran. The triplex PCR method was used to allocate the E. coli isolates based on the presence or absence of 3 genes (chuA, yjaA, and tspE4.C2) to yield 4 definite phylogenetic groups and 7 subgroups. Phenotypic ESBL-producing E. coli was determined using the double disk diffusion method according to the manufacturer's instructions and Clinical & Laboratory Standards Institute (CLSI) guidelines. Results: A total of 65.04% and 22.3% of isolates from calves and 70.5% and 20.5% of isolates from dairy cows belonged to phylogroups B1 and A, respectively. In addition, no isolate from the diarrhoeic calves was found to belong to group B2 and subgroups D2 and A0. A low prevalence (2/205 isolates, 0.97%) of ESBL-producing E. coli was found only in the samples of dairy cows which belonged to the phylogenetic group A and phylogenetic subgroup A1. There was no statistically significant relationship between the phylogenetic group and the production of ESBLs (P = 0.11). There was also no difference between the E. coli isolates from calves and dairy cows in the production of ESBLs (P = 0.5). Conclusion: There was no statistically significant relationship between the phylogenetic group and the production of ESBLs (P = 0.11). There was also no difference between E. coli isolates from calves and dairy cows in the production of ESBLs (P = 0.5). Based on these results, there is a low prevalence of ESBL-producing E. coli in the dairy farms of Khuzestan province. However, further large scale investigations are necessary to control the antibiotic resistance in the human and animal foodstuff.

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“…Wastewater treatment processes are designed to reduce the concentration of contaminants, including pathogens, in the effluent before discharge to receiving water bodies; however, many wastewater treatment plants (WWTPs) discharge such effluents without disinfection. Consequently, nondisinfected effluents could still contain high proportions of pathogenic bacteria, thus presenting a threat to public health (2).…”

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confidence: 99%

“…Previous environmental surveys to determine the natural prevalence of pathogenic E. coli relied on the detection of genes by PCR (2,7,8) and colony hybridizations (9,10) or on their phylogenetic classification (groups A, B1, B2, C, D, and E) (11,12). At least 567 virulence genes (VGs) have been reported for E. coli (13), which can be grouped into 78 virulence factors (VFs) and include functions such as adhesins, toxins, capsules, secretion systems, iron uptake systems, and invasins (14)(15)(16)(17)(18).…”

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confidence: 99%

Biological and Physicochemical Wastewater Treatment Processes Reduce the Prevalence of Virulent Escherichia coli

Frigon

Biswal

Mazza

et al. 2013

Appl Environ Microbiol

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bEffluents discharged from wastewater treatment plants are possible sources of pathogenic bacteria, including Escherichia coli, in the freshwater environment, and determining the possible selection of pathogens is important. This study evaluated the impact of activated sludge and physicochemical wastewater treatment processes on the prevalence of potentially virulent E. coli. A total of 719 E. coli isolates collected from four municipal plants in Québec before and after treatment were characterized by using a customized DNA microarray to determine the impact of treatment processes on the frequency of specific pathotypes and virulence genes. The percentages of potentially pathogenic E. coli isolates in the plant influents varied between 26 and 51%, and in the effluents, the percentages were 14 to 31%, for a reduction observed at all plants ranging between 14 and 45%. Pathotypes associated with extraintestinal pathogenic E. coli (ExPEC) were the most abundant at three of the four plants and represented 24% of all isolates, while intestinal pathogenic E. coli pathotypes (IPEC) represented 10% of the isolates. At the plant where ExPEC isolates were not the most abundant, a large number of isolates were classified as both ExPEC and IPEC; overall, 6% of the isolates were classified in both groups, with the majority being from the same plant. The reduction of the proportion of pathogenic E. coli could not be explained by the preferential loss of one virulence gene or one type of virulence factor; however, the quinolone resistance gene (qnrS) appears to enhance the loss of virulence genes, suggesting a mechanism involving the loss of pathogenicity islands.

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Phylogenetic groups, virulence genes and quinolone resistance of integron-bearing Escherichia coli strains isolated from a wastewater treatment plant

Cited by 48 publications

References 34 publications

Molecular Characterization and Antimicrobial Resistance of Uropathogenic Escherichia coli

Molecular Characterization and Antimicrobial Resistance of Uropathogenic Escherichia coli

Phylogenetic Grouping and Phenotypic Detection of Extended-Spectrum β-Lactamases Among Escherichia coli From Calves and Dairy Cows in Khuzestan, Iran

Biological and Physicochemical Wastewater Treatment Processes Reduce the Prevalence of Virulent Escherichia coli

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