Phylogenetic evidence for the transfer of Eubacterium lentum to the genus Eggerthella as Eggerthella lenta gen. nov., comb. nov.

Abstract

Phylogenetic evidence for the transfer ofEubacterium lentum has unique phenotypic characters within the genus Eubacterium. The 165 rRNA sequence of Eubaderium lentum was determined and its phylogenetic position was defined. This micro-organism is a member of the genus Eubacterium but it is not closely related to Eubacterium limosum, the type species of the genus Eubacterium, and is nearer to Collinsella aerofaciens and Coriobacterium glomerans. A PCR-based identification system using species-specif ic primers designed on the basis of DNA sequences encoding the 16s rRNA of strains of Eubacterium lentum, Collinsella aerofaciens and Coriobacterium glomerans is described. A species-specif ic primer set can distinguish Eubacterium lentum from Eubacterium limosum or close I y re I a ted species i ncl ud i ng Collinsella aero faciens, Coriobact erium glomerans and Atopobium species. This species-specif i c PCR method can be used to identify Eubacterium lentum-like species isolated from human faeces. On the basis of the 16s rRNA sequence divergence from Collinsella aerofaciens and Coriobacterium glomerans and the presence of unique phenotypic characters, a new genus, Eggerthella gen. nov., is proposed for Eubacterium lentum, with one species, Eggerthella lenta comb. nov. The type strain of Eggerthella lenta is JCM 997gT.

Dynamics and diversity of the ‘Atopobium cluster' in the human faecal microbiota, and phenotypic characterization of ‘Atopobium cluster' isolates

“…Thorasin, L. Hoyles and A. L. McCartney However, it is noteworthy that our Eggerthella API 20 A data clearly indicated that, with the exception of the type strain, all strains were saccharolytic. These findings contradict previously published data, including the species description of E. lenta, which suggest that E. lenta is generally unreactive in typical phenotypic laboratory tests and is asaccharolytic (Eggerth, 1935;Moore et al, 1971;Kageyama et al, 1999b;Wade et al, 1999). This most likely reflects the fact that all steps of the phenotypic characterization work performed in this study were carried out in an anaerobic cabinet, as opposed to manipulations being done on the laboratory bench and incubations in anaerobic gas jars (which take up to 1 h to reduce the atmospheric conditions if using gas-generating sachets).…”

Bifidobacterial Lipoglycan as a New Cause for False-Positive Platelia Aspergillus Enzyme-Linked Immunosorbent Assay Reactivity

“…The only nonbifidobacterial species that showed PA ELISA reactivity was Eubacterium lentum. This species, which is one of the predominant microorganisms from the human intestine, has been reclassified as Eggerthella lenta (19). In contrast to other eubacteria, which belong to the low-GϩC group, this bacterium has a high GϩC content and by definition a lipoglycan instead of an LTA (31).…”

Enantioselective Dehydroxylation of Enterodiol and Enterolactone Precursors by Human Intestinal Bacteria

“…lenta by phylogenic identification. 11) In this study, we determined phylogenic affiliation of E. sp. SDG-2 with 16S rRNA gene-based identification using polymerase chain reaction with proper primers.…”