Phylogenetic diversity of soda lake alkaliphiles

Abstract

16S rRNA genes from a range of aerobic chemoorganotrophic, alkaliphilic soda lake Bacteria and Archaea have been sequenced and subjected to phylogenetic analysis. Gram‐negative alkaliphiles were found to be confined to the γ3 subdivision of the Proteobacteria, with many isolates related to the Halomonas/Deleya group. Gram‐positive alkaliphiles were found in both high % G + C and low % G + C divisions of the Gram‐positive lineage, with many isolates being related to the Bacillus group, others to Arthrobacter spp. Alkaliphilic Archaea were relatively closely related to members of the genera Natronococcus and Natronobacterium. An anaerobic, thermophilic isolate has been assigned to a new genus within the Thermotogales.

Natrialba hulunbeirensis sp. nov. and Natrialba chahannaoensis sp. nov., novel haloalkaliphilic archaea from soda lakes in Inner Mongolia Autonomous Region, China.

“…In recent years, more and more complete 16S rRNA gene sequences have become available for the halobacteria. Phylogenetic analysis of 16S rRNA gene sequences has now shown considerable taxonomic diversity within the family Halobacteriaceae (Kamekura & DyallSmith, 1995 ;McGenity & Grant, 1995 ;Duckworth et al, 1996) and, to date, 15 genera have been described, including a number of genera where representative strains are all haloalkaliphilic and others that include some haloalkaliphilic types. …”

Planktonic bacterial community composition of an extremely shallow soda pond during a phytoplankton bloom revealed by cultivation and molecular cloning

“…Phylogenetic diversity of bacterial communities inhabiting athalassohaline lakes around the world has been examined in detail from the end of the twentieth century (Duckworth et al 1996;Jones et al 1998). Recently, the cultivation dependent and independent analyses of planktonic and sediment microbial communities include, among others, studying the soda lakes of South-East Siberia, Russia (Foti et al 2007(Foti et al , 2008, North-East Mongolia (Sorokin et al 2004), Inner Mongolia, China (Zhang et al 2001;Ma et al 2004a), Lonar Lake, India (Wani et al 2006;Joshi et al 2008), the East African Rift Valley, Kenya and Tanzania (Rees et al 2004;Mwirichia et al 2010Mwirichia et al , 2011, Wadi An Natrun, Egypt (Mesbah et al 2007), as well as Mono Lake, California, USA (Hollibaugh et al 2001;Humayoun et al 2003) and Soap Lake, Washington, USA (Dimitru et al 2008).…”

Bacterial diversity and activity along a salinity gradient in soda lakes of the Kulunda Steppe (Altai, Russia)

“…In the last decade, special attention has been given to the investigation of the microbial communities in soda lakes using both traditional isolation methods (Duckworth et al 1996;Sorokin et al 2004;Sorokin and Kuenen 2005b) and molecular biology techniques (Humayoun et al 2003;Rees et al 2004;Scholten et al 2005;Foti et al 2007). Few reviews (Zavarzin et al 1999;Jones et al 1998) summarize these results, showing that soda lakes contain representatives of the major trophic and phylogenetic groups of prokaryotes, and that they can be considered as autonomous systems, in which cycling of nutrients is close to complete.…”

“…Few reviews (Zavarzin et al 1999;Jones et al 1998) summarize these results, showing that soda lakes contain representatives of the major trophic and phylogenetic groups of prokaryotes, and that they can be considered as autonomous systems, in which cycling of nutrients is close to complete. The most well-studied soda lakes are those located in the East African Rift Valley (Duckworth et al 1996;Rees et al 2004), in the Libyan desert (Wadi Natrun) (Imhoff et al 1979) and in North America, i.e. Mono Lake (California) (Humayoun et al 2003;Scholten et al 2005a) and Soap Lake (Washington) (Sorokin et al 2007a).…”